Editorial focus: entering into the non-coding RNA era

Bartoszewski, Rafał; Sikorski, Aleksander F.

doi:10.1186/s11658-018-0111-3

Cited by 39 publications

(35 citation statements)

References 114 publications

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“…Finally, numerous reports have illustrated the large diversity in terms of responses and clinical outcomes to COVID-19 infections. One possible variable that has not been considered is the individual differences in patient’s miRNA profiles or polymorphisms in either MTSs or miRNA sequences ( 9 , 11 , 56 , 73 ). In that regard, a recent study has suggested that COVID-19 virulence in aged patients may be due to a lower abundance of miRNAs and this may be a contributing factor in disease severity ( 34 ).…”

Section: Comparison Of the Mirna Target Sites In Human Coronavirusesmentioning

confidence: 99%

SARS-CoV-2 may regulate cellular responses through depletion of specific host miRNAs

Bartoszewski

Dąbrowski

Jakieła

et al. 2020

American Journal of Physiology-Lung Cellular and Molecular Phys

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Cold viruses have generally been considered fairly innocuous until the appearance of the severe acute respiratory coronavirus 2 (SARS-CoV-2) in 2019 which caused the coronavirus disease 2019 (COVID-19) global pandemic. Two previous viruses foreshadowed that a coronavirus could potentially have devastating consequences in 2002 (severe acute respiratory coronavirus (SARS-CoV)) and in 2012 (middle east respiratory syndrome coronavirus (MERS-CoV)). The question that arises is why these viruses are so different from the relatively harmless cold viruses. Based on an analysis of the current literature and using bioinformatic approaches, we examined the potential human miRNA interactions with the SARS-CoV-2's genome, and compared the miRNA target sites in seven coronavirus genomes that include SARS-CoV-2, MERS-CoV, SARS-CoV, and four non-pathogenic coronaviruses. Here, we discuss the possibility that pathogenic HCoVs including SARS-CoV-2 could modulate host miRNA levels by acting as miRNA sponges to facilitate viral replication and/or to avoid immune responses.

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Section: Comparison Of the Mirna Target Sites In Human Coronavirusesmentioning

confidence: 99%

SARS-CoV-2 may regulate cellular responses through depletion of specific host miRNAs

Bartoszewski

Dąbrowski

Jakieła

et al. 2020

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…MicroRNAs (miRNAs) have become an object of intense research in biomedical sciences as they play an important role in the regulation of complex biological systems by repressing gene translation 1–3 . For example, miRNAs are involved in inflammatory processes and have been linked to endothelial dysfunction 4–6 and macrophage polarization 7–9 .…”

Section: Introductionmentioning

confidence: 99%

Identification of stably expressed housekeeping miRNAs in endothelial cells and macrophages in an inflammatory setting

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Krohn

Schümann

2019

Sci Rep

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Reliable quantification of miRNA expression by qRT-PCR crucially depends on validated housekeepers for data normalization. Here we present thoroughly tested miRNAs eligible as references in immunological studies utilizing endothelial cells and macrophages, respectively. Endothelial cells (cell line: TIME) and macrophages (cell line: RAW264.7) were treated with various pro- and anti-inflammatory mediators (cytokines, LPS, unsaturated fatty acids) given as either single substances or in combination. Isolated RNA was screened for stably expressed miRNAs by next generation sequencing. Housekeeper candidates were thereafter validated by means of two independent quantification techniques: qRT-PCR for relative quantification and ddPCR for absolute quantification. Both methods consistently confirmed the suitability of let-7g-5p, let-7i-5p, miR-127-3p and miR-151a-5p in cytokine/fatty acid-treated TIME and miR-16-5p, miR-27b-3p, miR-103a-3p and miR-423-3p in LPS/fatty acid-treated RAW264.7, respectively as housekeeping miRNAs. With respect to abundancy and over all expression stability the miRNAs miR-151a-5p (cell line: TIME) as well as miR-27b-3p and miR-103a-3p (cell line: RAW264.7) can be particularly recommended for normalization of qRT-PCR data.

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“…Besides the expression of coding RNAs, non-coding RNAs (ncRNAs) such as small and long non-coding RNAs (sncRNAs, lncRNAs) can play an important role during viral infections [ 25 ]. ncRNAs are known as post-transcriptional regulators [ 26 ], and they can participate in virus-host interactions through a variety of mechanisms [ 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1

et al. 2020

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Background Since 2008, the aquaculture production of Crassostrea gigas was heavily affected by mass mortalities associated to Ostreid herpesvirus 1 (OsHV-1) microvariants worldwide. Transcriptomic studies revealed the major antiviral pathways of the oyster immune response while other findings suggested that also small non-coding RNAs (sncRNA) such as microRNAs might act as key regulators of the oyster response against OsHV-1. To explore the explicit connection between small non-coding and protein-coding transcripts, we performed paired whole transcriptome analysis of sncRNA and messenger RNA (mRNA) in six oysters selected for different intensities of OsHV-1 infection. Results The mRNA profiles of the naturally infected oysters were mostly governed by the transcriptional activity of OsHV-1, with several differentially expressed genes mapping to the interferon, toll, apoptosis, and pro-PO pathways. In contrast, miRNA profiles suggested more complex regulatory mechanisms, with 15 differentially expressed miRNAs (DE-miRNA) pointing to a possible modulation of the host response during OsHV-1 infection. We predicted 68 interactions between DE-miRNAs and oyster 3′-UTRs, but only few of them involved antiviral genes. The sncRNA reads assigned to OsHV-1 rather resembled mRNA degradation products, suggesting the absence of genuine viral miRNAs. Conclusions We provided data describing the miRNAome during OsHV-1 infection in C. gigas. This information can be used to understand the role of miRNAs in healthy and diseased oysters, to identify new targets for functional studies and, eventually to disentangle cause and effect relationships during viral infections in marine mollusks.

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Editorial focus: entering into the non-coding RNA era

Cited by 39 publications

References 114 publications

SARS-CoV-2 may regulate cellular responses through depletion of specific host miRNAs

SARS-CoV-2 may regulate cellular responses through depletion of specific host miRNAs

Identification of stably expressed housekeeping miRNAs in endothelial cells and macrophages in an inflammatory setting

Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1

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