EDC/NHS cross-linked collagen foams as scaffolds for artificial corneal stroma

Vrana, Nihal Engin; Builles, Nicolas; Koçak, Hülya; Gulay, P.; Justin, V.; Malbouyres, Marilyne; Ruggiero, Florence; Damour, O.; Hasırcı, Vasıf

doi:10.1163/156856207794761961

Cited by 53 publications

(41 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mixture of EDC and NHS were also effectively used for cross-linking of collagen scaffolds derived from bovine and marine origin. 21,43 Further, the EDC/NHS cross-linked collagen was reported to be non-cytotoxic in vitro, 44 and biocompatibility was also demonstrated in animal models 45 as the unreacted EDC/NHS can be washed off in aqueous media. The developed cross-linked scaffolds containing nano/micro architecture may have resemblance to the intricate fibrillar structure of native tissue, where collagen directly supports cell adhesion by providing the chemical cues to cell surface receptors.…”

Section: Resultsmentioning

confidence: 99%

Collagen scaffolds derived from fresh water fish origin and their biocompatibility

Pati

Datta

Adhikari

et al. 2012

J Biomedical Materials Res

102

View full text Add to dashboard Cite

Collagen, a major component of native extracellular matrix, has diverse biomedical applications. However, its application is limited due to lack of cost-effective production and risk of disease transmission from bovine sources currently utilized. This study describes fabrication and characterization of nano/micro fibrous scaffolds utilizing collagen extracted from fresh water fish origin. This is the first time collagen extracted from fresh water fish origin was studied for their biocompatibility and immunogenicity. The nano/micro fibrous collagen scaffolds were fabricated through self-assembly owing to its amphiphilic nature and were subsequently cross-linked. In vitro degradation study revealed higher stability of the cross-linked scaffolds with only ~50% reduction of mass in 30 days, while the uncross-linked one degraded completely in 4 days. Further, minimal inflammatory response was observed when collagen solution was injected in mice with or without adjuvant, without significant dilution of sera. The fish collagen scaffolds exhibited considerable cell viability and were comparable with that of bovine collagen. SEM and fluorescence microscopic analysis revealed significant proliferation rate of cells on the scaffolds and within 5 days the cells were fully confluent. These findings indicated that fish collagen scaffolds derived from fresh water origin were highly biocompatible in nature.

show abstract

Section: Resultsmentioning

confidence: 99%

Collagen scaffolds derived from fresh water fish origin and their biocompatibility

Pati

Datta

Adhikari

et al. 2012

J Biomedical Materials Res

102

View full text Add to dashboard Cite

show abstract

“…[10][11][12][13][14][15][16][17][18][19][20][21] However, there is the need to improve collagen mechanical properties and rate of enzymatic degradation, thus leading to the employment of crosslinking procedures. 4,22,23 There are several methods to crosslink collagen, but the chemical methods are the most efficient. Agents such as glutaraldehyde, formaldehyde and diisocyanates introduce crosslinks between two e-amino groups of lysine and/or hydroxylysine residues and characterize the chemical crosslinking.…”

Section: Introductionmentioning

confidence: 99%

“…Crosslinking of collagen using N-(3-dimethylaminopropyl)-N 0 -ethylcarbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS) was shown to be non-cytotoxic in vitro and its biocompatibility was previously demonstrated. 4,[22][23][24][25] To obtain a scaffold that meets the criteria described previously, a highly porous HA scaffold was obtained using the polymer replication method. 26 To improve its biological properties and mimic the bone composition, collagen type I was coated onto the surface of the ceramic scaffold.…”

Section: Introductionmentioning

confidence: 99%

In vivo evaluation of highly macroporous ceramic scaffolds for bone tissue engineering

Teixeira

Fernandes

Leusink

et al. 2009

J Biomedical Materials Res

View full text Add to dashboard Cite

During the last decades, different materials of both natural and synthetic origin have been developed with the aim of inducing and controlling osteogenic differentiation of mesenchymal stem cells (MSCs). In order for that to happen, it is necessary that the material to be implanted obey a series of requirements, namely: osteoconduction, biocompatibility, and biodegradability. Additionally, they must be low-priced, easy to produce, shape, and store. Hydroxyapatite (HA) is a well known ceramic with a composition similar to the mineral component of bone and is highly biocompatible and easy to obtain and/or process. On the other hand, collagen is the main structural protein present in the human body and bone. In this study, a polymer replication method was applied and a highly porous HA scaffold was produced. Collagen was later incorporated to improve the biological properties of the scaffold while resembling the bone composition. The scaffolds were characterized by means of scanning electron microscopy, Fourier transform infrared spectroscopy and energy dispersive spectroscopy. In vitro and in vivo testing was performed in all scaffolds produced. The goal of this study was to evaluate the in vivo osteogenic potential of MSCs from two different species seeded on the different HA basedporous scaffolds with collagen type I. The resultsindicate that all scaffolds exhibit relevant bone formation, being more prominent in the case of the HA scaffolds.

show abstract

“…Collagen sponges were investigated for their use in cornea tissue engineering. After optimizing the degree of cross-linking, the sponges were seeded with human corneal keratocytes and characterized in vitro for use in potential reconstitution of corneal stroma [78]. Immunohistochemical analysis showed that keratocytes populated the scaffold with cells migrating both longitudinally and laterally, and secreted some of the main constituents of the corneal ECM (collagen types I, V and VI) after a month of incubation.…”

Section: Collagen Scaffoldsmentioning

confidence: 99%

Biomaterials and tissue engineering research in Turkey: The METU Biomat Center experience

Zorlutuna¹,

Huri²,

Basmanav³

et al. 2009

Biotechnology Journal

View full text Add to dashboard Cite

EDC/NHS cross-linked collagen foams as scaffolds for artificial corneal stroma

Cited by 53 publications

References 31 publications

Collagen scaffolds derived from fresh water fish origin and their biocompatibility

Collagen scaffolds derived from fresh water fish origin and their biocompatibility

In vivo evaluation of highly macroporous ceramic scaffolds for bone tissue engineering

Biomaterials and tissue engineering research in Turkey: The METU Biomat Center experience

Contact Info

Product

Resources

About