Ectopic expression of the BoTFL1-like gene of Bambusa oldhamii delays blossoming in Arabidopsis thaliana and rescues the tfl1 mutant phenotype

Hy, Zeng; Lyu, Yongtao; Xm, Yang; Xu, Yan; Lin, Xin

doi:10.4238/2015.august.10.11

Cited by 15 publications

(10 citation statements)

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“…Our BLAST analyses identified five copies of Ph.TFL1 genes in P. heterocycla (Table 1). A functional TFL1 gene was isolated from B. oldhamii and was overexpressed in Arabidopsis (Zeng et al, 2015). The overexpressed lines showed delayed flowering, suggesting that TFL1 may have a role in maintaining vegetative growth.…”

Section: Possible Physiological and Genetic Factors Regulating Bamboomentioning

confidence: 99%

“…In comparison, the molecular aspects of bamboo flowering remain at a nascent stage. Studies have been conducted to characterize a limited number of flowering genes in different bamboo species such as MADS18 from Dendrocalamus latiflorus (Bo et al, 2005), FLOWERING LOCUS T (FT) from P. meyeri (Hisamoto et al, 2008), TERMINAL FLOWER 1 (TFL1) like gene from Bambusa oldhamii (Zeng et al, 2015), FRIGIDA (FRI) from P. violascens (Liu et al, 2015), MADS1 and MADS2 from P. praecox (Lin et al, 2009), 10 genes related to floral transition and meristem identity in D. latiflorus (Wang et al, 2014) and 16 MADS box genes from B. edulis (Shih et al, 2014). Such targeted approaches are being complemented by high-throughput approaches, namely, de novo transcriptome sequencing and suppression subtractive hybridization (Lin et al, 2010; Liu et al, 2012; Zhang et al, 2012; Peng et al, 2013; Gao et al, 2014; Ge et al, 2016; Wysocki et al, 2016; Zhao et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Bamboo Flowering from the Perspective of Comparative Genomics and Transcriptomics

et al. 2016

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Bamboos are an important member of the subfamily Bambusoideae, family Poaceae. The plant group exhibits wide variation with respect to the timing (1–120 years) and nature (sporadic vs. gregarious) of flowering among species. Usually flowering in woody bamboos is synchronous across culms growing over a large area, known as gregarious flowering. In many monocarpic bamboos this is followed by mass death and seed setting. While in sporadic flowering an isolated wild clump may flower, set little or no seed and remain alive. Such wide variation in flowering time and extent means that the plant group serves as repositories for genes and expression patterns that are unique to bamboo. Due to the dearth of available genomic and transcriptomic resources, limited studies have been undertaken to identify the potential molecular players in bamboo flowering. The public release of the first bamboo genome sequence Phyllostachys heterocycla, availability of related genomes Brachypodium distachyon and Oryza sativa provide us the opportunity to study this long-standing biological problem in a comparative and functional genomics framework. We identified bamboo genes homologous to those of Oryza and Brachypodium that are involved in established pathways such as vernalization, photoperiod, autonomous, and hormonal regulation of flowering. Additionally, we investigated triggers like stress (drought), physiological maturity and micro RNAs that may play crucial roles in flowering. We also analyzed available transcriptome datasets of different bamboo species to identify genes and their involvement in bamboo flowering. Finally, we summarize potential research hurdles that need to be addressed in future research.

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Section: Possible Physiological and Genetic Factors Regulating Bamboomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bamboo Flowering from the Perspective of Comparative Genomics and Transcriptomics

et al. 2016

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“…Some species have a lengthy vegetative stage that may last more than 120 years while other species flower after only 1 year. Little is known about floral induction in bamboo or the genes involved in the process [ 6 , 15 – 17 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of PvCO1, a bamboo CONSTANS-LIKE gene, delays flowering by reducing expression of the FT gene in transgenic Arabidopsis

Gao

Chen

et al. 2018

BMC Plant Biol

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BackgroundIn Arabidopsis, a long day flowering plant, CONSTANS (CO) acts as a transcriptional activator of flowering under long day (LD) condition. In rice, a short day flowering plant, Hd1, the ortholog of CO, plays dual functions in respond to day-length, activates flowering in short days and represses flowering in long days. In addition, alleles of Hd1 account for ~ 44% of the variation in flowering time observed in cultivated rice and sorghum. How does it work in bamboo? The function of CO in bamboo is similar to that in Arabidopsis?ResultsTwo CO homologous genes, PvCO1 and PvCO2, in Phyllostachys violascens were identified. Alignment analysis showed that the two PvCOLs had the highest sequence similarity to rice Hd1. Both PvCO1 and PvCO2 expressed in specific tissues, mainly in leaf. The PvCO1 gene had low expression before flowering, high expression during the flowering stage, and then declined to low expression again after flowering. In contrast, expression of PvCO2 was low during the flowering stage, but rapidly increased to a high level after flowering. The mRNA levels of both PvCOs exhibited a diurnal rhythm. Both PvCO1 and PvCO2 proteins were localized in nucleus of cells. PvCO1 could interact with PvGF14c protein which belonged to 14–3-3 gene family through B-box domain. Overexpression of PvCO1 in Arabidopsis significantly caused late flowering by reducing the expression of AtFT, whereas, transgenic plants overexpressing PvCO2 showed a similar flowering time with WT under LD conditions. Taken together, these results suggested that PvCO1 was involved in the flowering regulation, and PvCO2 may either not have a role in regulating flowering or act redundantly with other flowering regulators in Arabidopsis. Our data also indicated regulatory divergence between PvCOLs in Ph. violascens and CO in Arabidopsis as well as Hd1 in Oryza sativa. Our results will provide useful information for elucidating the regulatory mechanism of COLs involved in the flowering.ConclusionsUnlike to the CO gene in Arabidopsis, PvCO1 was a negative regulator of flowering in transgenic Arabidopsis under LD condition. It was likely that long period of vegetative growth of this bamboo species was related with the regulation of PvCO1.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1469-0) contains supplementary material, which is available to authorized users.

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“…Many flowering promoters in bamboo have been reported ( Tian et al, 2005 ; Lin et al, 2009 , 2010 ; Guo et al, 2016 ; Liu et al, 2016a ). We previously showed that BoTFL1-like and PvFRIL might be possible floral suppressors of bamboo ( Zeng et al, 2015 ; Liu et al, 2016c ). In this study, we identified and characterized another possible floral suppressor, a Pin1-like gene from P. violascens named PvPin1.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of PvPin1, a Bamboo Homolog of PIN1-Type Parvulin 1, Delays Flowering Time in Transgenic Arabidopsis and Rice

Zhang

Yang

et al. 2017

Front. Plant Sci.

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Because of the long and unpredictable flowering period in bamboo, the molecular mechanism of bamboo flowering is unclear. Recent study showed that Arabidopsis PIN1-type parvulin 1 (Pin1At) is an important floral activator and regulates floral transition by facilitating the cis/trans isomerization of the phosphorylated Ser/Thr residues preceding proline motifs in suppressor of overexpression of CO 1 (SOC1) and agamous-like 24 (AGL24). Whether bamboo has a Pin1 homolog and whether it works in bamboo flowering are still unknown. In this study, we cloned PvPin1, a homolog of Pin1At, from Phyllostachys violascens (Bambusoideae). Bioinformatics analysis showed that PvPin1 is closely related to Pin1-like proteins in monocots. PvPin1 was widely expressed in all tested bamboo tissues, with the highest expression in young leaf and lowest in floral bud. Moreover, PvPin1 expression was high in leaves before bamboo flowering then declined during flower development. Overexpression of PvPin1 significantly delayed flowering time by downregulating SOC1 and AGL24 expression in Arabidopsis under greenhouse conditions and conferred a significantly late flowering phenotype by upregulating OsMADS56 in rice under field conditions. PvPin1 showed subcellular localization in both the nucleus and cytolemma. The 1500-bp sequence of the PvPin1 promoter was cloned, and cis-acting element prediction showed that ABRE and TGACG-motif elements, which responded to abscisic acid (ABA) and methyl jasmonate (MeJA), respectively, were characteristic of P. violascens in comparison with Arabidopsis. On promoter activity analysis, exogenous ABA and MeJA could significantly inhibit PvPin1 expression. These findings suggested that PvPin1 may be a repressor in flowering, and its delay of flowering time could be regulated by ABA and MeJA in bamboo.

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Ectopic expression of the BoTFL1-like gene of Bambusa oldhamii delays blossoming in Arabidopsis thaliana and rescues the tfl1 mutant phenotype

Cited by 15 publications

References 37 publications

Bamboo Flowering from the Perspective of Comparative Genomics and Transcriptomics

Bamboo Flowering from the Perspective of Comparative Genomics and Transcriptomics

Overexpression of PvCO1, a bamboo CONSTANS-LIKE gene, delays flowering by reducing expression of the FT gene in transgenic Arabidopsis

Overexpression of PvPin1, a Bamboo Homolog of PIN1-Type Parvulin 1, Delays Flowering Time in Transgenic Arabidopsis and Rice

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