Ectopic expression of TaYAB1, a member of YABBY gene family in wheat, causes the partial abaxialization of the adaxial epidermises of leaves and arrests the development of shoot apical meristem in Arabidopsis

Wang, Zhao; Su, Hong; Song, Jian; Zhao, Xiang; Zhang, Xian Sheng

doi:10.1016/j.plantsci.2005.09.008

Cited by 36 publications

(29 citation statements)

References 26 publications

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“…After pretreatment, slides were hybridized with 200 ng/mL probes at 47°C overnight in hybridization solution containing 50% formamide. The detection of hybridized signals was carried out as described by Zhao et al (2006).…”

Section: In Situ Hybridizationmentioning

confidence: 99%

The expression of Orysa;CycB1;1 is essential for endosperm formation and causes embryo enlargement in rice

Guo

Wang

Song

et al. 2009

Planta

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The cell cycle is an important process during seed development in plants and its progression is driven by a number of core regulators such as the cyclins. Currently, however, little is known regarding the role of the cyclins in embryo and endosperm development in cereals. In our current study, we show that the knockdown of Orysa;CycB1;1 in rice results in the production of abnormal seeds, which at maturity contain only an enlarged embryo. It was further found that a delayed and abnormal cellularization occurred in the endosperm in these knockdown seeds which eventually became abortive. Moreover, the observed development of the enlarged embryo was also morphologically abnormal and found to be caused by an enlarged cell size rather than an increased cell number. Expression analysis showed that Orysa;CycB1;1 transcripts were localized in the endosperm and embryo. Genome-wide transcriptional profiling further indicated that a large number of genes are responsible for the phenotype of the enlarged embryo. The results of the knockdown of Orysa;CycB1;1 via an endosperm or an embryo-specific promoter also suggest that the enlarged embryo may be correlated to the abortive endosperm. Our results suggest that Orysa;CycB1;1 expression is critical for endosperm formation via the regulation of mitotic division, and that the endosperm plays an important role in maintenance of embryo development in rice.

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Section: In Situ Hybridizationmentioning

confidence: 99%

The expression of Orysa;CycB1;1 is essential for endosperm formation and causes embryo enlargement in rice

Guo

Wang

Song

et al. 2009

Planta

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“…In Arabidopsis, the YABBY genes are largely expressed in the abaxial region of lateral organs and are involved in specifying abaxial cell fate in general; by contrast, the function of the YABBY genes is likely to have diversified in the angiosperms as assessed from the varied expression patterns of these genes among various species (Yamada et al, 2003(Yamada et al, , 2004Jang et al, 2004;Juarez et al, 2004;Fourquin et al, 2005;Zhao et al, 2006;Toriba et al, 2007). The CRABS CLAW (CRC) gene, an ortholog of DL, is required for nectary development and is partially involved in carpel identity (Alvarez and Smyth, 1999;Bowman and Smyth, 1999;Baum et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

The spatial expression patterns of DROOPING LEAF orthologs suggest a conserved function in grasses

et al. 2009

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The DROOPING LEAF (DL) gene regulates carpel specification in the flower and midrib formation in the leaf in Oryza sativa (rice). Loss-of-function mutations in the dl locus cause homeotic transformation of carpels into stamens and lack of midrib, resulting in the drooping leaf phenotype. DL is a member of the YABBY gene family and is closely related to the CRABS CLAW (CRC) gene in Arabidopsis thaliana. The function of Arabidopsis CRC, however, differs from that of rice DL: it is responsible for nectary development and is partially involved in carpel identity. Thus, genes related to DL/CRC seem to have functionally diversified during angiosperm evolution. To assess the conservation of DL function in related species, here we examined the in situ expression patterns of DL orthologs in three grass species, i.e., maize, wheat and sorghum, which is assigned to subfamilies different from Ehrhartoideae including O. sativa. The results clearly show that the temporal and spatial expression patterns of DL orthologs in the three species are identical to those of rice DL in both flower and leaf development, suggesting that DL-related genes are functionally conserved within the grass family. It is likely that DL may have been recruited to carpel specification and midrib formation within the lineage of the grass family after divergence of their ancestor from that of eudicots.

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“…Remarkably, zyb9 and zyb14, belonging to the FIL/YAB3 clade, are expressed in the adaxial region of leaf primordia in Zea mays and may be unrelated to speciWcation of polar cell fate (Juarez et al 2004b). YABBY genes in Oryza sativa (rice) and Triticum aestivum (wheat) are not expressed in a polar manner (Jang et al 2004;Zhao et al 2006). In basal angiosperms, a YABBY2 ortholog is expressed in the adaxial region of the leaf and carpel primordia in A. trichopoda, like maize yab9 and yab14, whereas a CRC ortholog of A. trichopoda and an INO ortholog of Nymphaea alba are expressed in a polar manner similar to that of the corresponding YABBY gene of Arabidopsis (Yamada et al 2003(Yamada et al , 2004Fourquin et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization the YABBY gene family in Oryza sativa and expression analysis of OsYABBY1

et al. 2007

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Members of the YABBY gene family have a general role that promotes abaxial cell fate in a model eudicot, Arabidopsis thaliana. To understand the function of YABBY genes in monocots, we have isolated all YABBY genes in Oryza sativa (rice), and revealed the spatial and temporal expression pattern of one of these genes, OsYABBY1. In rice, eight YABBY genes constitute a small gene family and are classified into four groups according to sequence similarity, exon-intron structure, and organ-specific expression patterns. OsYABBY1 shows unique spatial expression patterns that have not previously been reported for other YABBY genes, so far. OsYABBY1 is expressed in putative precursor cells of both the mestome sheath in the large vascular bundle and the abaxial sclerenchyma in the leaves. In the flower, OsYABBY1 is specifically expressed in the palea and lemma from their inception, and is confined to several cell layers of these organs in the later developmental stages. The OsYABBY1-expressing domains are closely associated with cells that subsequently differentiate into sclerenchymatous cells. These findings suggest that the function of OsYABBY1 is involved in regulating the differentiation of a few specific cell types and is unrelated to polar regulation of lateral organ development.

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Ectopic expression of TaYAB1, a member of YABBY gene family in wheat, causes the partial abaxialization of the adaxial epidermises of leaves and arrests the development of shoot apical meristem in Arabidopsis

Cited by 36 publications

References 26 publications

The expression of Orysa;CycB1;1 is essential for endosperm formation and causes embryo enlargement in rice

The expression of Orysa;CycB1;1 is essential for endosperm formation and causes embryo enlargement in rice

The spatial expression patterns of DROOPING LEAF orthologs suggest a conserved function in grasses

Molecular characterization the YABBY gene family in Oryza sativa and expression analysis of OsYABBY1

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