The spatial expression patterns of DROOPING LEAF orthologs suggest a conserved function in grasses

Ishikawa, Motokazu; Ohmori, Yuki; Tanaka, Wakana; Hirabayashi, Chizuru; Murai, Koji; Ogihara, Yasunari; Yamaguchi, Takahiro; Hirano, Hideki

doi:10.1266/ggs.84.137

Cited by 56 publications

(59 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To examine temporal and spatial patterns of drl1 and drl2 transcript accumulation during midrib, leaf, and shoot development, we performed RNA in situ hybridization. In median longitudinal and transverse sections through normal shoot apices, we detected drl1 and drl2 transcripts specifically in the medial region of the incipient (P0) and emergent (P1-P4) leaf primordia, but not in the SAM or adjacent developing internodes (Figures 6A to 6C and 6G to 6I) (Brooks et al, 2009;Ishikawa et al, 2009). Importantly, drl1 and drl2 transcripts were detected in precursors of adaxial clear cells and abaxial hypodermal sclerenchyma, cell types that are affected in the mature leaf by mutations in the drl genes, but not in the midvein provascular strand at P1-P2, which gives rise to cell types that are unaffected.…”

Section: The Drl Genes Are Expressed Early In Leaf Primordia and Inflmentioning

confidence: 96%

Maize YABBY Genes drooping leaf1 and drooping leaf2 Regulate Plant Architecture

et al. 2017

View full text Add to dashboard Cite

Leaf architecture directly influences canopy structure, consequentially affecting yield. We discovered a maize (Zea mays) mutant with aberrant leaf architecture, which we named drooping leaf1 (drl1). Pleiotropic mutations in drl1 affect leaf length and width, leaf angle, and internode length and diameter. These phenotypes are enhanced by natural variation at the drl2 enhancer locus, including reduced expression of the drl2-Mo17 allele in the Mo17 inbred. A second drl2 allele, produced by transposon mutagenesis, interacted synergistically with drl1 mutants and reduced drl2 transcript levels. The drl genes are required for proper leaf patterning, development and cell proliferation of leaf support tissues, and for restricting auricle expansion at the midrib. The paralogous loci encode maize CRABS CLAW co-orthologs in the YABBY family of transcriptional regulators. The drl genes are coexpressed in incipient and emergent leaf primordia at the shoot apex, but not in the vegetative meristem or stem. Genome-wide association studies using maize NAM-RIL (nested association mapping-recombinant inbred line) populations indicated that the drl loci reside within quantitative trait locus regions for leaf angle, leaf width, and internode length and identified rare single nucleotide polymorphisms with large phenotypic effects for the latter two traits. This study demonstrates that drl genes control the development of key agronomic traits in maize.

show abstract

Section: The Drl Genes Are Expressed Early In Leaf Primordia and Inflmentioning

confidence: 96%

Maize YABBY Genes drooping leaf1 and drooping leaf2 Regulate Plant Architecture

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Rice DL belongs to the CRABS CLAW (CRC)/DL subfamily of the YABBY transcription factors, is expressed in the entire carpel primordium, and loss-of-function mutations cause homeotic conversion of carpels to stamens (Yamaguchi et al, 2004). The expression pattern of DL orthologs was found to be conserved in four grass species (rice, maize, wheat, and sorghum); therefore, DL function is likely to be conserved within the grass family (Ishikawa et al, 2009). A DL ortholog was isolated from the monocot Asparagus asparagoides, designated AaDL, and this gene is expressed specifically in the abaxial side of the developing carpels (Nakayama et al, 2010).…”

Section: -4) Grass Flowersmentioning

confidence: 99%

Molecular Mechanism Regulating Floral Architecture in Monocotyledonous Ornamental Plants

Kanno

2016

Hortic J

View full text Add to dashboard Cite

Molecular and genetic analyses of flower development have been conducted primarily in dicot model plants such as Arabidopsis thaliana and Antirrhinum majus. The obtained data are the basis for the ABC model, which was extended to the ABCE model of floral development. This model has been validated in many dicot species using genetic transformation studies and mutant analyses. Many dicot flowers have two distinctive perianth whorls, which include greenish sepals and showy petals. By contrast, the monocot lily flower has two almost identical petaloid whorls, the inner and outer tepals. To explain this type of floral morphology, a modified ABC model, the further extended modified ABCE model, was proposed. According to this model, B-class genes are expressed in whorls 1-3, and whorls 1 and 2 form petaloid structures. This review describes the molecular mechanisms regulating flower development in monocots. Since the showy perianth is one of the most important traits in floricultural crops, we focused on the B-and C-class genes, which are related to the development and modification of the perianth. The review describes the expression patterns of floral organ identity genes, and presents functional studies using double-flowered and viridiflora cultivars in some monocot species. Besides lily-type flowers, there are several types of monocot flower, such as commelina type with two whorls of distinctive perianth, orchid and grass flowers. The review also describes the molecular analyses of these types of monocot flower.

show abstract

“…DL is a member of the YABBY gene family (see Supplemental Figure 5A and Supplemental Data Set 4 online) and has a unique function in monocot bifacial leaves, such as those of rice (Yamaguchi et al, 2004;Ishikawa et al, 2009). In rice, DL is expressed at the center of leaves, where it regulates the formation of the leaf midrib, a rigid and thickened structure at the center of the leaf, through a function to promote cell proliferation of leaf primordia toward the SAM side (Yamaguchi et al, 2004).…”

Section: Is Strongly Expressed In Flattened Unifacial Leaf Primordmentioning

confidence: 99%

“…Since DL orthologs function in leaf development in monocots alone (Bowman and Smyth, 1999;Yamaguchi et al, 2004;Fourquin et al, 2005;Ishikawa et al, 2009;Wang et al, 2009), monocot leaves may possess the unique ability to become flattened by escaping from a developmental constraint to be radialized, even when they are abaxialized. Thus, the specific function of DL in leaf development in monocots may account for one genetic background that has allowed the repeated evolution of unifacial leaves in monocots.…”

Section: Evolution Of Leaf Flattening In Unifacial Leavesmentioning

confidence: 99%

Genetic Framework for Flattened Leaf Blade Formation in Unifacial Leaves ofJuncus prismatocarpus

2010

Self Cite

View full text Add to dashboard Cite

Angiosperm leaves generally develop as bifacial structures with distinct adaxial and abaxial identities. However, several monocot species, such as iris and leek, develop unifacial leaves, in which leaf blades have only abaxial identity. In bifacial leaves, adaxial-abaxial polarity is required for leaf blade flattening, whereas many unifacial leaves become flattened despite their leaf blades being abaxialized. Here, we investigate the mechanisms underlying the development and evolution of flattened leaf blades in unifacial leaves. We demonstrate that the unifacial leaf blade is abaxialized at the gene expression level and that an ortholog of the DROOPING LEAF (DL) gene may promote flattening of the unifacial leaf blade. In two closely related Juncus species, Juncus prismatocarpus, which has flattened unifacial leaves, and Juncus wallichianus, which has cylindrical unifacial leaves, DL expression levels and patterns correlate with the degree of laminar outgrowth. Genetic and expression studies using interspecific hybrids of the two species reveal that the DL locus from J. prismatocarpus flattens the unifacial leaf blade and expresses higher amounts of DL transcript than does that from J. wallichianus. We also show that leaf blade flattening is a trigger for central-marginal leaf polarity differentiation. We suggest that flattened unifacial leaf blades may have evolved via the recruitment of DL function, which plays a similar cellular but distinct phenotypic role in monocot bifacial leaves.

show abstract

The spatial expression patterns of DROOPING LEAF orthologs suggest a conserved function in grasses

Cited by 56 publications

References 39 publications

Maize YABBY Genes drooping leaf1 and drooping leaf2 Regulate Plant Architecture

Maize YABBY Genes drooping leaf1 and drooping leaf2 Regulate Plant Architecture

Molecular Mechanism Regulating Floral Architecture in Monocotyledonous Ornamental Plants

Genetic Framework for Flattened Leaf Blade Formation in Unifacial Leaves ofJuncus prismatocarpus

Contact Info

Product

Resources

About