Ectopic expression of subunit A of vacuolar H+-ATPase from apple enhances salt tolerance in tobacco plants

Dong, Qinglong; Liu, D. D.; Wang, Q. J.; Fang, Mou-Jing; Yao, Yuxin

doi:10.1134/s1021443715050064

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…Drought stress affects cotton yield, but the mechanism of drought resistance in cotton has not been revealed. Previous research found transgenic tobacco overexpression of apple VHA-A gene significantly increased dehydration resistance and salt tolerance ( Dong et al, 2013 , 2015 ). However, it is still not clear whether cotton VHA-A is involved in drought stress response.…”

Section: Discussionmentioning

confidence: 95%

The Gene Encoding Subunit A of the Vacuolar H+-ATPase From Cotton Plays an Important Role in Conferring Tolerance to Water Deficit

et al. 2018

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In plant cells, vacuolar H+-ATPases (V-ATPases) are responsible for deacidification of the cytosol and energisation of the secondary transport processes across the tonoplast. A number of V-ATPase subunit genes have been demonstrated to be involved in the regulation of the plant response to water deficit. However, there are no reports on the role of V-ATPase subunit A (VHA-A) in dehydration tolerance of cotton. In this study, cotton GhVHA-A gene was functionally characterized, especially with regard to its role in dehydration stress tolerance. Expression analysis showed that GhVHA-A was differentially expressed in various cotton organs and was induced by dehydration, low temperature, high salinity, and abscisic acid treatment in leaves. We also report that GhVHA-A improve dehydration tolerance in transgenic tobacco and cotton. Virus-induced gene silencing of GhVHA-A decreased the tolerance of cotton plantlets to dehydration stress. Silencing GhVHA-A decreased chlorophyll content and antioxidant enzyme activities and increased malondialdehyde (MDA) content in cotton under dehydration stress. However, transgenic tobacco expressing GhVHA-A exhibited enhanced dehydration resistance, resulting in reduced leaf water loss, higher average root length, and lower MDA levels under dehydration stress. Meanwhile, overexpression of GhVHA-A in tobacco conferred water deficit tolerance by enhancing osmotic adjustment (proline) and the activities of the antioxidant enzymes superoxide dismutase and peroxidase, thereby enhancing reactive oxygen species detoxification. These results suggest that GhVHA-A plays an important role in conferring resistance to dehydration stress. Our results have identified GhVHA-A as a candidate gene for improving dehydration tolerance in plants.

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Section: Discussionmentioning

confidence: 95%

The Gene Encoding Subunit A of the Vacuolar H+-ATPase From Cotton Plays an Important Role in Conferring Tolerance to Water Deficit

et al. 2018

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“…These A. thaliana transgenic lines had higher Na + (119%) and K + (183%) concentrations in shoots than non-transformed wild-type plants, which have been associated by those authors with greater capacity for vacuolar compartmentation. For example, stem height of transgenic N. tabacum plants overexpressing MdVHA-A, as compared to non-transformed wild-type plants, was similar in the non-saline controls, but it was 126-139% at 100 mM, 116-136% at 200 mM and 126-136% at 300 mM NaCl (Dong et al, 2015). The transgenic lines with greater growth in NaCl had also higher Na + concentration in leaves in all salinity treatments but the leaf K + concentrations were only significantly higher in lines grown at 100 mM NaCl.…”

Section: Vacuolar H + -Atpasementioning

confidence: 96%

“…Improvement in salinity tolerance of transgenic plants expressing different VHA subunits was, in most cases, attributed to a presumed enhanced vacuolar Na + or K + compartmentation due to increased activity of V-H + ATPase. In addition to the effects of VHA transgenes on tissue ions, overexpressing VHA subunits can also increase the activities of enzymatic antioxidants, such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) (Dabbous et al, 2017), and decrease the levels of malondialdehyde (Dabbous et al, 2017;Dong et al, 2015). Several other reports indicated also the effects of transgenes on increased activities of oxidative defence system enzymes; however, these studies did not assess tissue ion concentra-…”

Section: Vacuolar H + -Atpasementioning

confidence: 99%

Improving crop salt tolerance using transgenic approaches: An update and physiological analysis

Kotula

Caparrós

Zörb

et al. 2020

Plant Cell & Environment

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Salinization of land is likely to increase due to climate change with impact on agricultural production. Since most species used as crops are sensitive to salinity, improvement of salt tolerance is needed to maintain global food production. This review summarises successes and failures of transgenic approaches in improving salt tolerance in crop species. A conceptual model of coordinated physiological mechanisms in roots and shoots required for salt tolerance is presented. Transgenic plants overexpressing genes of key proteins contributing to Na + 'exclusion' (PM-ATPases with SOS1 antiporter, and HKT1 transporter) and Na + compartmentation in vacuoles (V-H + ATPase and V-H + PPase with NHX antiporter), as well as two proteins potentially involved in alleviating water deficit during salt stress (aquaporins and dehydrins), were evaluated. Of the 51 transformations, with gene(s) involved in Na + 'exclusion' or Na + vacuolar compartmentation that contained quantitative data on growth and include a non-saline control, 48 showed improvements in salt tolerance (less impact on plant mass) of transgenic plants, but with only two tested in field conditions. Of these 51 transformations, 26 involved crop species. Tissue ion concentrations were altered, but not always in the same way. Although glasshouse data are promising, field studies are required to assess crop salinity tolerance.

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Recombinant DNA Technology for Sustainable Plant Growth and Production

Pandey

2021

Harsh Environment and Plant Resilience

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Ectopic expression of subunit A of vacuolar H+-ATPase from apple enhances salt tolerance in tobacco plants

Cited by 6 publications

References 29 publications

The Gene Encoding Subunit A of the Vacuolar H+-ATPase From Cotton Plays an Important Role in Conferring Tolerance to Water Deficit

The Gene Encoding Subunit A of the Vacuolar H+-ATPase From Cotton Plays an Important Role in Conferring Tolerance to Water Deficit

Improving crop salt tolerance using transgenic approaches: An update and physiological analysis

Recombinant DNA Technology for Sustainable Plant Growth and Production

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