The Gene Encoding Subunit A of the Vacuolar H+-ATPase From Cotton Plays an Important Role in Conferring Tolerance to Water Deficit

Liu, Na; Zhang, Haiyan; Chen, Quanjia; Gao, Wenwei; Cai, Yongsheng; Li, Mengyu; Sun, Guixin; Qu, Yanying

doi:10.3389/fpls.2018.00758

Cited by 27 publications

(16 citation statements)

References 49 publications

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“…Wang et al (2016a) showed that overexpression of a vacuolar-type HC-ATPase C subunit gene from Iris lactea (IrlVHA-c), enhanced the salt tolerance in tobacco. Liu et al (2018) demonstrated that the gene encoding subunit A of the vacuolar H + -ATPase from cotton (GhVHA-A) plays an important role in conferring tolerance to water deficit. The above studies also infer that the genes isolated from halophytes could be effectively utilized for alleviating salt stress in the crop plants.…”

Section: Vacuolar Membrane H + -Atpase (V-atpase)mentioning

confidence: 99%

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Engineering salinity tolerance in plants: progress and prospects

Wani

Kumar

Khare

et al. 2020

Planta

149

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Soil salinity exerts significant constraints on global crop production, posing a serious challenge for plant breeders and biotechnologists. The classical transgenic approach for enhancing salinity tolerance in plants revolves by boosting endogenous defence mechanisms, often via a single gene 2 approach, and usually involves the enhanced synthesis of compatible osmolytes, antioxidants, polyamines, maintenance of hormone homeostasis, modification of transporters and/or regulatory proteins, including transcription factors (TFs) and alternative splicing events. Occasionally, genetic manipulation of regulatory proteins or phytohormone levels confers salinity-tolerance, but all these may cause undesired reduction in plant growth and/or yields. In this review, we present and evaluate novel and cutting-edge approaches for engineering salt tolerance in crop plants. First, we cover recent findings regarding the importance of regulatory proteins and transporters, and how they can be used to enhance salt tolerance in crop plants. We also evaluate the importance of halobiomes as a reservoir of genes that can be used for engineering salt-tolerance in glycophytic crops. Additionally, the role of microRNAs as critical post-transcriptional regulators in plant adaptive responses to salt stress are reviewed and their use for engineering salt-tolerant crop plants is critically assessed. The potentials of alternative splicing mechanisms and targeted gene-editing technologies in understanding plant salt-stress responses and developing salt-tolerant crop plants is also discussed.

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Section: Vacuolar Membrane H + -Atpase (V-atpase)mentioning

confidence: 99%

“…It is now becoming evident through several reports of genome-wide association studies that single base-pair changes constitute much of the variation in elite traits (Zhang et al 2018).…”

Section: Plant Genome Editing For Producing Salt-tolerant Plantsmentioning

confidence: 99%

Engineering salinity tolerance in plants: progress and prospects

Wani

Kumar

Khare

et al. 2020

Planta

149

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“…Studies have shown that V-ATPase is involved in multiple stress responses [10,18,19]. qPCR analysis showed that AtVHA-d2 was significantly induced by multiple stresses, especially oxidative stress (Fig.…”

Section: Discussionmentioning

confidence: 95%

Arabidopsis V-ATPase d2 subunit plays a role in plant responses to oxidative stress

Feng

Peng

Liu

et al. 2020

Preprint

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Background: Vacuolar-type H + -ATPase (V-ATPase) is a multisubunit proton pump located on the endomembranes, which plays an important role in plant growth. The Arabidopsis V-ATPase d subunit consists of two isoforms, AtVHA-d1 and AtVHA-d2. Results: In this study, the function of the AtVHA-d2 gene was investigated. Histochemical analysis revealed that the AtVHA-d1 and AtVHA-d2 genes were generally and highly overlapping expressed in multiple tissues at different developmental stages of Arabidopsis. Subcellular localization showed that AtVHA-d2 was mainly localized to the vacuole. The AtVHA-d2 expression was significantly induced by oxidative stress. Furthermore, the phenotypic analysis showed that the atvha-d2 mutant was sensitive to oxidative stress. The non-invasive micro-test measurement demonstrated that the net H + influx in the atvha-d2 roots was weaker than that of the wild type under normal conditions. However, oxidative stress resulted in the H + efflux in atvha-d2 roots, which was significantly different from the wild type. RNA-seq combined with qPCR analysis showed that the expression of several members of the plasma membrane H + -ATPase gene ( AtAHA ) family in atvha-d2 were significant different from wild type under normal and oxidative stress. Conclusion: Overall, our results indicate that AtVHA-d2 plays a role in Arabidopsis in response to oxidative stress by affecting H + flux and AtAHA gene expression.

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“…Studies have shown that V-ATPase is involved in multiple stress responses [12,23,31]. The expression of AtVHA-d genes was investigated under abiotic stress conditions that significantly inhibited the growth of Arabidopsis seedlings, including 150 mM NaCl, 300 mM mannitol, 5 mM H 2 O 2 , 100 µM ABA, cold (4 • C), and heat (37 • C).…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis V-ATPase d2 Subunit Plays a Role in Plant Responses to Oxidative Stress

Feng

Peng

Liu

et al. 2020

Genes

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Vacuolar-type H+-ATPase (V-ATPase), a multisubunit proton pump located on the endomembrane, plays an important role in plant growth. The Arabidopsis thaliana V-ATPase d subunit (VHA-d) consists of two isoforms; AtVHA-d1 and AtVHA-d2. In this study, the function of AtVHA-d2 was investigated. Histochemical analysis revealed that the expression of AtVHA-d1 and AtVHA-d2 was generally highly overlapping in multiple tissues at different developmental stages of Arabidopsis. Subcellular localization revealed that AtVHA-d2 was mainly localized to the vacuole. AtVHA-d2 expression was significantly induced by oxidative stress. Analysis of phenotypic and H2O2 content showed that the atvha-d2 mutant was sensitive to oxidative stress. The noninvasive microtest monitoring demonstrated that the net H+ influx in the atvha-d2 roots was weaker than that in the wild-type under normal conditions. However, oxidative stress resulted in the H+ efflux in atvha-d2 roots, which was significantly different from that in the wild-type. RNA-seq combined with qPCR analysis showed that the expression of several members of the plasma membrane H+-ATPase gene (AtAHA) family in atvha-d2 was significantly different from that in the wild-type. Overall, our results indicate that AtVHA-d2 plays a role in Arabidopsis in response to oxidative stress by affecting H+ flux and AtAHA gene expression.

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The Gene Encoding Subunit A of the Vacuolar H+-ATPase From Cotton Plays an Important Role in Conferring Tolerance to Water Deficit

Cited by 27 publications

References 49 publications

Engineering salinity tolerance in plants: progress and prospects

Engineering salinity tolerance in plants: progress and prospects

Arabidopsis V-ATPase d2 subunit plays a role in plant responses to oxidative stress

Arabidopsis V-ATPase d2 Subunit Plays a Role in Plant Responses to Oxidative Stress

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