Ectopic expression of sex peptide alters reproductive behavior of female D. melanogaster

Aigaki, Toshiro; Fleischmann, Iréne; Chen, Pei-Shen; Kubli, Eric

doi:10.1016/0896-6273(91)90368-a

Cited by 244 publications

(212 citation statements)

References 34 publications

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“…This construct contains the signal peptide resulting in secretion of SP. In wild-type females, delivery of SP to neurons reduces receptivity and induces oviposition indistinguishably from delivery to the genital tract by mating or to the haemolymph by secretion of an ectopically expressed SP gene in the fat body (figure 1a,b) [5]. In SPR mutant females, neuronal expression of SP can induce the full response in reducing receptivity and inducing oviposition ( figure 1a,b).…”

Section: Resultsmentioning

confidence: 97%

“…repoGAL4 is an enhancer-trap insert in the repo gene. For transgenic delivery of SP to the haemolymph via secretion from fat body expression under the yp1 promoter, a YPhsSPg insert on the second chromosome was used [5] and mSP was expressed from an insert on the third chromosome [23]. fruGal4 and dsxGal4 are Gal4 insertions into the endogenous locus, and for expression in the ppk and GMR, patterns inserts on the second chromosome were used [21,27,28].…”

Section: Methodsmentioning

confidence: 99%

“…SP leads to rejection of courting males and increases egg production and oviposition [4][5][6][7][8], but also leads to other behavioural and physiological changes. These include an increase in feeding, a change in food choice and sleep, and stimulation of the immune system [9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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Multiple pathways mediate the sex-peptide-regulated switch in female Drosophila reproductive behaviours

et al. 2013

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Male-derived sex-peptide (SP) induces profound changes in the behaviour of Drosophila females, resulting in decreased receptivity to further mating and increased egg laying. SP can mediate the switch in female reproductive behaviours via a G protein-coupled receptor, SPR, in neurons expressing fruitless, doublesex and pickpocket. Whether SPR is the sole receptor and whether SP induces the postmating switch in a single pathway has not, to our knowledge been tested. Here we report that the SP response can be induced in the absence of SPR when SP is ectopically expressed in neurons or when SP, transferred by mating, can access neurons through a leaky blood brain barrier. Membrane-tethered SP can induce oviposition via doublesex, but not fruitless and pickpocket neurons in SPR mutant females. Although pickpocket and doublesex neurons rely on G(o) signalling to reduce receptivity and induce oviposition, G(o) signalling in fruitless neurons is required only to induce oviposition, but not to reduce receptivity. Our results show that SP's action in reducing receptivity and inducing oviposition can be separated in fruitless and doublesex neurons. Hence, the SP-induced postmating switch incorporates shared, but also distinct circuitry of fruitless, doublesex and pickpocket neurons and additional receptors.

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Section: Resultsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

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Multiple pathways mediate the sex-peptide-regulated switch in female Drosophila reproductive behaviours

et al. 2013

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“…We do not know if Ae. aegypti mRGPs enter the gut of the mated female, but in D. melanogaster, of the 16 mRGPs examined, 12 enter the hemolymph following mating (Monsma et al, 1990;Ravi Ram et al, 2005;Aigaki et al, 1991;Ravi Ram and Wolfner, unpublished data;Meikle et al, 1990;Lung and Wolfner, 1999;Coleman et al, 1995). Therefore, it is also possible that mRGPs transferred to female mosquitoes could play a role in partitioning or binding digestive products after they are absorbed through the midgut.…”

Section: 5f Iron Regulation and Blood Clotting-since Femalementioning

confidence: 99%

Identity and transfer of male reproductive gland proteins of the dengue vector mosquito, Aedes aegypti: Potential tools for control of female feeding and reproduction

Sirot

Poulson

McKenna

et al. 2008

Insect Biochemistry and Molecular Biology

152

166

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Male reproductive gland proteins (mRGPs) impact the physiology and/or behavior of mated females in a broad range of organisms. We sought to identify mRGPs of the yellow fever mosquito, Aedes aegypti, the primary vector of dengue and yellow fever viruses. Earlier studies with Ae. aegypti demonstrated that "matrone" (a partially purified male reproductive accessory gland substance) or male accessory gland fluid injected into virgin female Ae. aegypti affect female sexual refractoriness, blood feeding and digestion, flight, ovarian development, and oviposition. Using bioinformatic comparisons to Drosophila melanogaster accessory gland proteins and mass spectrometry of proteins from Ae. aegypti male accessory glands and ejaculatory ducts (AG/ED) and female reproductive tracts, we identified 63 new putative Ae. aegypti mRGPs. Twenty-one of these proteins were found in the reproductive tract of mated females, but not of virgin females, suggesting that they are transferred from males to females during mating. Most of the putative mRGPs fall into the same protein classes as mRGPs in other organisms, although some appear to be evolving rapidly and lack identifiable homologs in Culex pipiens, Anopheles gambiae, and D. melanogaster. Our results identify candidate male-derived molecules that may have an important influence on behavior, survival and reproduction of female mosquitoes.

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“…Different adaptive features would operate between unmated and mated females in the natural populations. Because molecules such as sex-peptides and others which are transmitted from male to female during copulation influence oviposition and remating of mated females (Chen and Bühler, 1970;Aigaki et al, 1991;Fuyama and Ueyama, 1997;Wolfner, 1997), I speculate that these molecules may mediate the decrease of emigration activity in mated females. Although it was suggested that the emigration activities of the sexes were under different genetic control (Mikasa, 1992), the factors derived from males from copulation may not be ignored with respect to the variability of emigration activity in the mated females.…”

Section: Discussionmentioning

confidence: 99%

Intraspecific variation in the effects of mating on emigration activity and fecundity in a natural population of Drosophila melanogaster.

Mikasa

1998

Genes Genet. Syst.

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Intraspecific variation in the effects of mating on the emigration response behavior and fecundity of Drosophila melanogaster was investigated using isofemale lines of the Himeji population in Japan. The emigration activities of the mated and unmated females were examined with Sakai's population system. The isofemale lines were classified into two groups with respect to the effect of mating on emigration activity; 1) copulation decreased the emigration activity in 26 out of 28 isofemale lines, and 2) higher emigration activity was noted in the mated than in the unmated females in two lines. The percentage of expressed genotypic variance on emigration activity was higher in the unmated females than in the mated ones. Gregarious oviposition did not seem to be related to the decrease of emigration activity in the mated females.

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Ectopic expression of sex peptide alters reproductive behavior of female D. melanogaster

Cited by 244 publications

References 34 publications

Multiple pathways mediate the sex-peptide-regulated switch in female Drosophila reproductive behaviours

Multiple pathways mediate the sex-peptide-regulated switch in female Drosophila reproductive behaviours

Identity and transfer of male reproductive gland proteins of the dengue vector mosquito, Aedes aegypti: Potential tools for control of female feeding and reproduction

Intraspecific variation in the effects of mating on emigration activity and fecundity in a natural population of Drosophila melanogaster.

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