Ectomycorrhizal fungi of Pinus pinea L. in northeastern Spain

Rincón, Ana María; Álvarez, Isabel F.; Pera, Joan

doi:10.1007/s005720050245

Cited by 24 publications

(18 citation statements)

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“…The pH was adjusted to 5.8 using 1N HCl. The isolates were incubated at 22˚C for 15-20 days in the dark, and maintained onto MMN medium by transfer every three months (Rincon et al, 1999). It is possible that certain physiological characteristics of ectomycorrhizal fungi, especially those relating to their enzymatic activity, are altered as a result of in vitro cultivation (Cairney, 1999;Buscot et al, 2000).…”

Section: Fungal Materialsmentioning

confidence: 99%

Effects of temperature, pH and carbon and nitrogen sources on growth of in vitro cultures of ectomycorrhizal isolates from Pinus heldreichii forest

2016

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Aim of study: This study aims to provide basic information about physiological characteristics of isolates of Lactarius deliciosus (L.) Gray, Russula sanguinaria (Schumach.) Rauschert, Suillus collinitus (Fr) Kuntze, Suillus granulatus (L.) Rousell, Tricholoma batchii Gulden and Tricholoma imbricatum (Fr.) Kumm.Area of study: The isolates are obtained from Pinus heldreichii H. Christ forest in the south-eastern part of Montenegro. Material and methods:The isolates were molecularly characterised by internal transcribed spacer (ITS) sequencing and restriction fragment length polymorphism (RFLP) analysis. The effects of different temperatures (20, 22, 25°C), pHs (4, 4.5, 5.2, 5.8, 6.5, 7.5), and carbon (glucose, sucrose, dextrin, arabinose, xylose and starch) and nitrogen (NH 4 + , NO 3 -and protein) sources on their growth were examined under laboratory conditions.Main results: The studied factors established significant differences in the development of isolates. Isolates of R. sanguinaria, L. deliciosus and both Suillus, were characterised by faster growth at 22°C, while Tricholoma isolates grew faster at 25°C. S. granulatus, S. collinitus and T. imbticatum isolates grew well at lower pH values (4 -5.2), while L. deliciosus, R. sanguinaria and T. bachii exhibited faster growth at pHs between 5.8 and 6.5. The examined isolates were able to utilize various carbohydrates as carbon sources. The biggest mycelial growth was characterised for sucrose, then glucose, dextrin, arabinose, starch and xylose. They grew on all examined nitrogen sources, while the biggest mycelia growth was achieved on ammonium, followed by nitrate and protein. Those characteristics varied amongst the species.Research highlights: Information about physiological characteristics of Tricholoma, Lactarius, Russula, as well as Suillus, are sparse. Hence, the data obtained in this study could contribute to the understanding of their function in ecosystems.Keywords: Lactarius; Montenegro; physiology; RFLP analysis; Russula; Suillus; Tricholoma. Citation: Lazarević, J., Stojičić, D., Keča, N. (2016). Effects of temperature, pH and carbon and nitrogen sources on growth of in vitro cultures of ectomycorrrhizal isolates from Pinus heldreichii forest. Forest Systems, Volume 25, Issue 1, e048. http://dx.doi.

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Section: Fungal Materialsmentioning

confidence: 99%

Effects of temperature, pH and carbon and nitrogen sources on growth of in vitro cultures of ectomycorrhizal isolates from Pinus heldreichii forest

2016

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“…Collected sporocarps were dried at 35 • C for 72 h and kept in paper bags until use. Pure cultures of L. laccata (strain 127, collected under Quercus ilex L.) and P. tinctorius (strain 93, collected under Quercus suber L.) were isolated from fresh sporocarp tissue as previously described [32]. Voucher dry basidiomata and pure cultures were deposited in the herbarium and culture collection of DPV-IRTA (Barcelona, Spain).…”

Section: Fungal Materials and Production Of Fungal Inoculummentioning

confidence: 99%

“…The percentage of mycorrhizal seedlings was determined in each treatment. Ectomycorrhizal short roots (ECM) were identified according to morphological criteria as previously described [32][33][34]. Each seedling root was cut in 2-3 cm segments, and the percentage of ECM assessed by counting at least 200 randomly selected short roots under the stereomicroscope.…”

Section: Measured Parameters and Statistical Analysismentioning

confidence: 99%

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Influence de la méthode de fertilisation sur la mycorhization contrôlée de deux espèces de Pins méditerranéens

Rincón¹,

Parladé²,

Pera³

2007

Ann. For. Sci.

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-The influence of the fertilisation method: soluble (SF) vs. slow-release fertiliser (SRF) and of inoculation with Laccaria laccata (Scop.) Fr., Pisolithus tinctorius (Pers.) Coker & Couch and Melanogaster ambiguus (Vittad.) Tul & C. Tul. on ectomycorrhizal colonization and growth of Pinus pinea L. and Pinus pinaster Ait. was evaluated. For both pines, mycorrhization with L. laccata was not affected by the fertilisation method. Percentages of ectomycorrhizas (ECM) formed by P. tinctorius were dependent on the fertilisation method, the inoculum type (vegetative or spores) and the pine species involved. ECM formed by M. ambiguus were increased with fertilisation in both pines. Inoculation significantly improved P. pinea biomass when seedlings were fertilised with SRF whereas no effect was found in non-fertilised ones. For non-fertilised P. pinaster, inoculation with L. laccata and both inocula of P. tinctorius increased seedling biomass whereas fertilisation neutralised the fungal effect. Fertilisation increased P. pinea and P. pinaster biomass, independently of the inoculation treatment. Pour les deux pins, la mycorhization avec Laccaria laccata n'a pas été modifiée par la méthode de fertilisation. Le pourcentage d'ectomycorrhizes (ECM) formé by P. tinctorius dépendait de la méthode de fertilisation, su type d'inoculum (spores ou inoculum végétatif) et de l'espèce de pin. La fertilisation a augmenté les ECM produites par Melanogaster ambiguus chez les deux pins. L'inoculation a augmenté significativement la biomasse des semis de Pinus pinea lorsqu'ils ont été fertilisés avec SRF tandis qu'aucun effet n'a été trouvé pour les traitements non fertilisés. Pour les semis non fertilisés de Pinus pinaster, l'inoculation avec Laccaria laccata et avec les deux inoculums de Pisolithus tinctorius a augmenté la biomasse des semis tandis que la fertilisation a neutralisé l'effet de l'inoculation. La fertilisation a augmenté la biomasse de Pinus pinaster et de Pinus pinea indépendamment du traitement d'inoculation utilisé.Pinus pinea / Pinus pinaster / mycorhization contrôlée / champignon ectomycorhizien / pépinière de production de semis / fertilisation

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“…Seedling roots were washed free of substrate and ectomycorrhizas were identified according to morphological criteria derived from previous results obtained in synthesis and inoculum dosage trials [31,32]. Each seedling root was cut into 2-3 cm segments, and the percentage of ectomycorrhizal short roots was assessed by counting at least 200 randomly selected short roots under the stereomicroscope.…”

Section: Measured Parametersmentioning

confidence: 99%

Effects of ectomycorrhizal inoculation and the type of substrate on mycorrhization, growth and nutrition of containerised Pinus pinea L. seedlings produced in a commercial nursery

Rincón

Parladé²,

Pera³

2005

Ann. For. Sci.

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-Spore inocula of Melanogaster ambiguus (Vittad.) Tul. & C. Tul., Pisolithus tinctorius (Pers.) Coker & Couch, Rhizopogon luteolus Fr., Rhizopogon roseolus (Corda) Th. M. Fr. and Scleroderma verrucosum (Bull.) Pers., and two substrates (a mixture of peat-vermiculite and a mixture of peat-composted pine bark) were evaluated for producing mycorrhizal Pinus pinea L. seedlings in a commercial nursery in NE Spain. Inocula of all fungi were effective to obtain containerised mycorrhizal P. pinea seedlings although seedling mycorrhization rates were reduced in the peat/pine-bark mixture. Neither inoculation nor the type of substrate modified the first-year growth of seedlings. Rhizopogon spp. increased the levels of N and P and, in general inoculation reduced the concentration of Mn in needles. Symptoms of chlorosis were detected in all seedlings growing in the peat/pine-bark mixture, probably due to nutritional deficiencies caused by the substrate high pH. Among the fungi tested, R. roseolus is proposed as the best candidate for its application in nursery inoculation programmes destined to produced containerised mycorrhizal P. pinea. , et deux substrats (un mélange de tourbe-vermiculite et un mélange de tourbe-écorce compostée de pin) ont été évalués pour produire des jeunes plants mycorhizés de Pinus pinea L. dans une pépinière commerciale au Nord-Est de l'Espagne. Les inoculums de tous les champignons ont été efficaces pour obtenir des semis mycorhizés de pin pignon en conteneur, bien que les taux de mycorhization ont diminué dans le substrat vermiculite-écorce de pin. Ni l'inoculation ni le type de substrat n'ont modifié la croissance en première année des jeunes plants. Les espèces de Rhizopogon ont augmenté les niveaux d'azote et de phosphore dans les aiguilles des plants et, en général, l'inoculation a réduit la teneur en manganèse de ces feuilles. Des symptômes de chlorose ont été détectés dans tous les plants croissant dans le substrat vermiculiteécorce de pin, probablement en raison des carences nutritionnelles provoquées par le pH élevé du substrat. Parmi les champignons étudiés, on propose R. roseolus comme le meilleur candidat pour son application dans des programmes d'inoculation en pépinière destinés à produire des plants de pin pignon mycorhizés en conteneur.Pinus pinea / substrat / mycorhization / champignons ectomycorhiziens / production des semis en pépinière

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Ectomycorrhizal fungi of Pinus pinea L. in northeastern Spain

Cited by 24 publications

References 1 publication

Effects of temperature, pH and carbon and nitrogen sources on growth of in vitro cultures of ectomycorrhizal isolates from Pinus heldreichii forest

Effects of temperature, pH and carbon and nitrogen sources on growth of in vitro cultures of ectomycorrhizal isolates from Pinus heldreichii forest

Influence de la méthode de fertilisation sur la mycorhization contrôlée de deux espèces de Pins méditerranéens

Effects of ectomycorrhizal inoculation and the type of substrate on mycorrhization, growth and nutrition of containerised Pinus pinea L. seedlings produced in a commercial nursery

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