Ecotoxicological effects of trace element contamination in talitrid amphipod Orchestia montagui Audouin, 1826

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To estimate trace element bioaccumulation in Armadillo officinalis, specimens were collected from Ghar El Melh lagoon then exposed for 3 weeks in contaminated sediments with copper, zinc, and cadmium. From the first week until the end of the experiment, a decrease in A. officinalis growth related to the increase of Cd concentration in the sediment was recorded. However, a mass gain was highlighted under Cu and Zn exposures. At the end of experiment, body metal concentrations were measured using flame atomic emission spectrometry. Results of the bioaccumulation factor showed that the species could be considered as a macroconcentrator of copper (BAF > 2) and a deconcentrator of zinc (BAF < 2). Microscopy observations of hepatopancreas cells showed morphological and histological changes even at the lowest concentration. They consisted in the microvillus border destruction, lipid droplets modifications, trace element accumulation, and the condensation of the majority of cellular organelles. The degree of these alterations was found to be dosedependent. Through these results, the isopod A. officinalis could be used as relevant monitor organisms for soil metal contamination. K E Y W O R D S bioaccumulation, storage organ, terrestrial isopod, trace element, transmission electron microscopy

Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 52%

Evaluation of trace element contamination using Armadillo officinalis Duméril, 1816 (Crustacea, Isopoda) as a tool: An ultrastructural study

Khemaissia¹,

Jelassi²,

Ghemari³

et al. 2019

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“…Additionally, a condensation of the mitochondria was highlighted. This phenomenon is common for terrestrial isopods as well as other crustaceans groups such as Amphipoda (Jelassi, Ghemari, et al, ; Jelassi, Khemaissia, et al, ). Thus, it was reported that nickel exposure induced a marked swelling of the mitochondria in Porcellio scaber leading to mitochondrial dysfunctions with an increase of the nominal concentrations (Tarnawska, Migula, Przybylowicz, Mesjasz‐Przybylowicz, & Augustyniak, ).…”

Section: Discussionmentioning

confidence: 96%

“…The transmission electron microscopy was conducted according to the protocol used by Ayari, Raimond, Souty‐Grosset, and Nasri‐Ammar () and Jelassi, Ghemari, et al (); Jelassi, Khemaissia, et al (). Individuals were sacrificed at the end of exposure and immediately immersed in Ringer's saline solution.…”

Section: Methodsmentioning

confidence: 99%

“…Their tolerance to MTE is related to their physiological adaptations. Among them, their ability to compartmentalize metals in the granules of the hepatopancreas cells was highlighted (Jelassi, Ghemari, et al, ; Jelassi, Khemaissia, et al, ; Mazzei et al, ). Indeed, the hepatopancreas has been considered as the main storage organ of MTE accumulation (Hopkin & Martin, ).…”

Section: Introductionmentioning

confidence: 99%

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Physiological and histopathological responses of Porcellio laevis (Isopoda, Crustacea) as indicators of metal trace element contamination

Ghemari

Jelassi

Khemaissia

et al. 2020

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This study was designed to assess the impact of the mixture of cadmium (Cd) and zinc (Zn) on the bioaccumulation and the ultrastructural changes in the hepatopancreas of Porcellio laevis (Latreille, 1804) after 4 weeks of exposure to contaminated Quercus leaves under laboratory conditions. For each metal, four concentrations were used with four replicates for each concentration. Metal concentrations in the hepatopancreas and the rest of the body were determined using atomic absorption spectrometry. From the first week until the end of the experiment, a weight gain in P. laevis was observed particularly between the first and the end of exposure from 93.3 ± 18.22 mg fw to 105.22 ± 16.16 mg fw and from 106.4 ± 22.67 mg fw to 125.9 ± 23.9 mg fw for Mix1 and Mix4, respectively. Additionally, the determined metal trace elements (MTE) concentrations in the hepatopancreas were considerably higher compared to those in the rest of the body and seem to be dose‐dependent. Using transmission electron microscopy (TEM), some alterations were highlighted in the hepatopancreas. The main observed alterations were (a) the destruction of the microvilli border in a considerable portion of cells, (b) the increase of the lipid droplets with different shapes and sizes, (c) the increase in the number of the mitochondria, and (d) the appearance of TE in the form of B‐type granules. The obtained results confirmed the ability of P. laevis to deal with high amounts of MTE, suggesting its possible use in future soil's biomonitoring programs.

Effects of trace metal elements on ultrastructural features of hepatopancreas of Armadillidium granulatum Brandt, 1833 (Crustacea, Isopoda)

Khemaissia¹,

Jelassi²,

Ghemari³

et al. 2019

Self Cite

This study was conducted to compare metals bioaccumulation in the terrestrial isopod Armadillidium granulatum collected from Ghar El Melh lagoon. We focused on recognizing the effects of trace elements on hepatopancreas functional role. To this end, isopod specimens were exposed for 3 weeks to sediments contaminated with cadmium, copper, zinc, mercury, and nickel. Three concentrations were used in duplicate for each experimental condition. At the end of the experiment, metal body burdens were determined using flame atomic absorption spectrometry. Results of the bioaccumulation factor (BAF) showed that the species A. granulatum was classified as a Cu macroconcentrator (BAF > 2) and a Zn deconcentrator (BAF < 2). Dose dependent morphological and histological changes were observed in the hepatopancreas cells using transmission electron microscopy. The predominant features were: microvillus border disruption, condensation of the cytoplasm with increasing endoplasmic reticulum, mitochondria, lysosomes and granules that accumulated metals in B and S cells. The number of lipid droplets decreased especially after Cd, Zn, Hg, and Ni treatments. This study demonstrated that the terrestrial isopod A. gramulatum could be a good indicator of soil metal contamination.