Ecotoxicological Determination of Microplastic Toxicity on Algae Chlorella sp.: Response Surface Modeling Approach

Miloloža, Martina; Bule, Kristina; Ukić, Šime; Cvetnić, Matija; Bolanča, Tomislav; Kušić, Hrvoje; Grgić, Dajana Kučić

doi:10.1007/s11270-021-05267-0

Cited by 18 publications

(3 citation statements)

References 51 publications

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“…Thus, the bioavailability of the particles for biodegradation is better. However, according to previous studies, the toxic effect of MP increases with decreasing particle size [ 63 , 64 ].…”

Section: Resultsmentioning

confidence: 89%

Optimization of Polystyrene Biodegradation by Bacillus cereus and Pseudomonas alcaligenes Using Full Factorial Design

et al. 2022

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Microplastics (MP) are a global environmental problem because they persist in the environment for long periods of time and negatively impact aquatic organisms. Possible solutions for removing MP from the environment include biological processes such as bioremediation, which uses microorganisms to remove contaminants. This study investigated the biodegradation of polystyrene (PS) by two bacteria, Bacillus cereus and Pseudomonas alcaligenes, isolated from environmental samples in which MPs particles were present. First, determining significant factors affecting the biodegradation of MP-PS was conducted using the Taguchi design. Then, according to preliminary experiments, the optimal conditions for biodegradation were determined by a full factorial design (main experiments). The RSM methodology was applied, and statistical analysis of the obtained models was performed to analyze the influence of the studied factors. The most important factors for MP-PS biodegradation by Bacillus cereus were agitation speed, concentration, and size of PS, while agitation speed, size of PS, and optical density influenced the process by Pseudomonas alcaligenes. However, the optimal conditions for biodegradation of MP-PS by Bacillus cereus were achieved at γMP = 66.20, MP size = 413.29, and agitation speed = 100.45. The best conditions for MP-PS biodegradation by Pseudomonas alcaligenes were 161.08, 334.73, and 0.35, as agitation speed, MP size, and OD, respectively. In order to get a better insight into the process, the following analyzes were carried out. Changes in CFU, TOC, and TIC concentrations were observed during the biodegradation process. The increase in TOC values was explained by the detection of released additives from PS particles by LC-MS analysis. At the end of the process, the toxicity of the filtrate was determined, and the surface area of the particles was characterized by FTIR-ATR spectroscopy. Ecotoxicity results showed that the filtrate was toxic, indicating the presence of decomposition by-products. In both FTIR spectra, a characteristic weak peak at 1715 cm−1 was detected, indicating the formation of carbonyl groups (−C=O), confirming that a biodegradation process had taken place.

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Section: Resultsmentioning

confidence: 89%

Optimization of Polystyrene Biodegradation by Bacillus cereus and Pseudomonas alcaligenes Using Full Factorial Design

et al. 2022

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“…The model coefficients are denoted by a, while x 1 to x 3 represent the values of the three process parameters tested: pH, mass ratio of MPs to iron, and mass ratio of H 2 O 2 to iron. In addition to the linear (x 1 , x 2 , x 3 ) and quadratic (x 1 2 , x 2 2 , x 3 2 ) contributions, the model also includes the interaction terms (x 1 x 2 , x 1 x 3 , x 2 x 3 ) to cover the possible combined effects of the parameters tested, which, as we knew from experience [53][54][55][56][57], are not uncommon in such systems. The area of a broad FTIR band that occurred in the spectral range 3000-3600 cm −1 was used as the response, i.e., as the dependent variable y.…”

Section: Determination Of Optimal Conditionsmentioning

confidence: 99%

Evaluation of Fenton, Photo-Fenton and Fenton-like Processes in Degradation of PE, PP, and PVC Microplastics

Bule Možar,

Miloloža,

Martinjak

et al. 2024

Water

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The global problem of microplastics in the environment is “inspiring” scientists to find environmentally friendly and economically viable methods to remove these pollutants from the environment. Advanced oxidation processes are among the most promising methods. In this work, the potential of Fenton, photo-Fenton, and Fenton-like processes for the degradation of microplastics from low-density polyethylene (LDPE), polypropylene (PP), and poly(vinyl chloride) (PVC) in water suspensions was investigated. The influence of three parameters on the efficiency of the degradation process was tested: the pH of the medium (3–7), the mass of added iron (10–50 times less than the mass of microplastics), and the mass of added H2O2 (5–25 times more than the mass of added iron). The effectiveness of the treatment was monitored by FTIR-ATR spectroscopy. After 60-min treatments, the PP microparticles were found to be insensitive. In the Fenton treatment of PVC and the photo-Fenton treatment of LDPE and PVC, changes in the FTIR spectra related to the degradation of the microplastics were observed. In these three cases, the treatment parameters were optimized. It was found that a low pH (3) and a high iron mass (optimal values were 1/12 and 1/10 of the mass of the microplastics for LDPE and PVC, respectively) favored all three. The degradation of LDPE by the photo-Fenton treatment was favored by high H2O2 concentrations (25 times higher than the mass of iron), while these concentrations were significantly lower for PVC (11 and 15 times for the Fenton and photo-Fenton treatment, respectively), suggesting that scavenging activity occurs.

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“…Microplastic pollution in the aquatic environment is a global catastrophe and is widely distributed across various aquatic habitats worldwide, from seawater [ 1 , 5 , 6 , 97 ], marine sediment [ [7] , [8] , [9] , [10] , [11] ], beach sand [ [12] , [13] , [14] , [15] , [16] , [17] ], freshwater [ [18] , [19] , [20] , [21] ] riverine sediments [ [22] , [23] , [24] , [25] ] and lakes [ [26] , [27] , [28] ].…”

Section: Introductionmentioning

confidence: 99%

Microplastics in the Volta Lake: Occurrence, distribution, and human health implications

Boateng,

Addo,

Duodu

et al. 2024

Heliyon

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Ecotoxicological Determination of Microplastic Toxicity on Algae Chlorella sp.: Response Surface Modeling Approach

Cited by 18 publications

References 51 publications

Optimization of Polystyrene Biodegradation by Bacillus cereus and Pseudomonas alcaligenes Using Full Factorial Design

Optimization of Polystyrene Biodegradation by Bacillus cereus and Pseudomonas alcaligenes Using Full Factorial Design

Evaluation of Fenton, Photo-Fenton and Fenton-like Processes in Degradation of PE, PP, and PVC Microplastics

Microplastics in the Volta Lake: Occurrence, distribution, and human health implications

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