Economical, Plain, and Rapid Authentication of <i>Actaea racemosa</i>
 L. (syn. <i>Cimicifuga racemosa</i>
, Black Cohosh) Herbal Raw Material by Resilient RP-PDA-HPLC and Chemometric Analysis

Bittner, Marian; Schenk, Regina; Springer, Andreas; Melzig, Matthias F.

doi:10.1002/pca.2622

Cited by 13 publications

(8 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The HPLC-UV fingerprint of them has been used to distinguish A. racemosa from major American and Asian Actaea species [11, 21]. The current study demonstrated that the EIC profile in MS positive mode of these five hydroxycinnamic acid esters could also distinguish A. racemosa from the other two American species and two Asian adulterants successfully, as shown in Fig.…”

Section: Resultssupporting

confidence: 52%

“…The previous work of ours [19, 20] and other researchers [6, 8, 11, 14, 16, 18, 21–23] on black cohosh components focused primarily on: 1) using ESI-negative mode to detect phenolic acids and phenolic esters; 2) using ESI/APCI-positive mode to detect triterpenes. In order to explore useful information that may be neglected in the MS positive data, principle component analysis (PCA) was performed on positive data (especially compounds eluted ahead of triterpenes on reverse phase column) in this study.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Feruloyl dopamine-O-hexosides are efficient marker compounds as orthogonal validation for authentication of black cohosh (Actaea racemosa)—an UHPLC-HRAM-MS chemometrics study

et al. 2017

View full text Add to dashboard Cite

Due to the complexity and variation of the chemical constituents in authentic black cohosh (Actaea racemosa) and its potential adulterant species, an accurate and feasible method for black cohosh authentication is not easy. A high resolution accurate mass (HRAM) LC-MS fingerprinting method combined with chemometric approach was employed to discover new marker compounds. Seven hydroxycinnamic acid amide (HCAA) glycosides are proposed as potential marker compounds for differentiation of black cohosh from related species, including two Asian species (A. foetida, A. dahurica) and two American species (A. pachypoda, A. podocarpa). These markers were putatively identified by comparing their mass spectral fragmentation behavior with those of their authentic aglycone compounds and phytochemistry reports. Two isomers of feruloyl methyldopamine 4-O-hexoside ([M+H]+ 506) and one feruloyl tyramine 4-O-hexoside ([M+H]+ 476) contributed significantly to the separation of Asian species in principle component analysis (PCA) score plot. The efficacy of the models built on four reasonable combinations of these markers in differentiating black cohosh and its adulterants were evaluated and validated by partial least-square discriminant analysis (PLS-DA). Two models based on these reduced dataset achieved 100% accuracy based on the current sample collection, including the model that used only three feruloyl dopamine-O-hexoside isomers ([M+H]+ 492) and one feruloyl dopamine-O-dihexoside ([M+H-hexosyl]+ at m/z 492).

show abstract

Section: Resultssupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Feruloyl dopamine-O-hexosides are efficient marker compounds as orthogonal validation for authentication of black cohosh (Actaea racemosa)—an UHPLC-HRAM-MS chemometrics study

et al. 2017

View full text Add to dashboard Cite

show abstract

“…2 A) and an LC/ELSD method (▶ Fig. 2 B) according to the monograph of "Black Cohosh" in the USP [27,38] Fig. 3.…”

Section: Resultsmentioning

confidence: 99%

“…For LC analyses, 400 mg of the powdered plant material were accurately weighed and extracted by ultrasonication (ultrasonic bath Sonorex RK100H, Bandelin). In the case of the hydrolysis-sensitive polyphenols, the extraction time was kept short: 5 min at 60°C in 70 % (v/v) methanol followed by centrifugation at 4°C, filtration (0.22 µm), and storage in brown glass vials at 2-8°C until analysis the same day, as previously specified in [38]. For the assay on triterpene glycosides, a quantitative extraction procedure was applied in accordance to the USP monography on black cohosh.…”

Section: Lc Analyses Of Metabolitesmentioning

confidence: 99%

See 1 more Smart Citation

NIR Spectroscopy of Actaea racemosa L. rhizome – En Route to Fast and Low-Cost Quality Assessment

et al. 2017

Self Cite

View full text Add to dashboard Cite

Rhizomes of L. (formerly) gained increasing interest as a plant-derived drug due to its hormone-like activity and the absence of estrogenic activity. According to the Current Good Manufacturing Practices guidelines and pharmacopeial standards, quality assessment of herbal starting materials includes tests on identity and substitution, as well as quantification of secondary metabolites, usually by HPTLC and LC methods. To reduce the laboratory effort, we investigated near-infrared spectroscopy for rapid species authentication and quantification of metabolites of interest.Near-infrared spectroscopy analysis is carried out directly on the milled raw plant material. Spectra were correlated with reference data of polyphenols and triterpene glycosides determined by LC/diode array detection and LC/evaporative light scattering detection, respectively. Quantification models were built and validated by cross-validation procedures. Clone plants, derived by vegetative propagation, and plants of a collection from different geographical origins cultivated in Berlin were analysed together with mixed batches from wild harvests purchased at wholesalers.Generally, good to excellent correlations were found for the overall content of polyphenols with coefficients of determination of R > 0.93. For individual polyphenols such as fukinolic acid, only models containing clone plants succeeded (R > 0.92). For the total content of triterpene glycosides, results were generally worse in comparison to polyphenols and were observed only for the mixed batches (R = 0.93).Next to quantitative analysis, near-infrared spectroscopy was proven as a rapid alternative to other, more laborious methods for species authentication. Near-infrared spectroscopy was able to distinguish different spp. such as the North American and the Asian , and.

show abstract

Ethnobotany, Phytochemistry, Traditional and Modern Uses of Actaea racemosa L. (Black cohosh): A Review

Salari

Amiri

Ramezani

et al. 2021

Pharmacological Properties of Plant-Derived Natural Products and Implications for Human Health

View full text Add to dashboard Cite

Economical, Plain, and Rapid Authentication of Actaea racemosa L. (syn. Cimicifuga racemosa , Black Cohosh) Herbal Raw Material by Resilient RP-PDA-HPLC and Chemometric Analysis

Cited by 13 publications

References 28 publications

Feruloyl dopamine-O-hexosides are efficient marker compounds as orthogonal validation for authentication of black cohosh (Actaea racemosa)—an UHPLC-HRAM-MS chemometrics study

Feruloyl dopamine-O-hexosides are efficient marker compounds as orthogonal validation for authentication of black cohosh (Actaea racemosa)—an UHPLC-HRAM-MS chemometrics study

NIR Spectroscopy of Actaea racemosa L. rhizome – En Route to Fast and Low-Cost Quality Assessment

Ethnobotany, Phytochemistry, Traditional and Modern Uses of Actaea racemosa L. (Black cohosh): A Review

Contact Info

Product

Resources

About