Ecological role of bacterial inoculants and their potential impact on soil microbial diversity

Ambrosini, Adriana; Souza, Rocheli de; Passaglia, Luciane Maria Pereira

doi:10.1007/s11104-015-2727-7

Cited by 130 publications

(77 citation statements)

References 136 publications

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“…From individual inoculations in the beginning, either bacteria or fungi, to mixed inoculations having both bacteria and fungi yielded desirable results in some crops grown under certain soil and environmental conditions. However, the microbial applications did not always perform to expected levels under different ecological conditions even if the host was the same (Ambrosini et al, 2016). Perhaps singular or combination of two microbes were not able to establish in the soil resulting in below par effectiveness of the bioinoculants.…”

Section: Conventional Selection Breeding Focused On Plant Genomementioning

confidence: 99%

Microbiome Selection Could Spur Next-Generation Plant Breeding Strategies

2016

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“No plant is an island too…”Plants, though sessile, have developed a unique strategy to counter biotic and abiotic stresses by symbiotically co-evolving with microorganisms and tapping into their genome for this purpose. Soil is the bank of microbial diversity from which a plant selectively sources its microbiome to suit its needs. Besides soil, seeds, which carry the genetic blueprint of plants during trans-generational propagation, are home to diverse microbiota that acts as the principal source of microbial inoculum in crop cultivation. Overall, a plant is ensconced both on the outside and inside with a diverse assemblage of microbiota. Together, the plant genome and the genes of the microbiota that the plant harbors in different plant tissues, i.e., the ‘plant microbiome,’ form the holobiome which is now considered as unit of selection: ‘the holobiont.’ The ‘plant microbiome’ not only helps plants to remain fit but also offers critical genetic variability, hitherto, not employed in the breeding strategy by plant breeders, who traditionally have exploited the genetic variability of the host for developing high yielding or disease tolerant or drought resistant varieties. This fresh knowledge of the microbiome, particularly of the rhizosphere, offering genetic variability to plants, opens up new horizons for breeding that could usher in cultivation of next-generation crops depending less on inorganic inputs, resistant to insect pest and diseases and resilient to climatic perturbations. We surmise, from ever increasing evidences, that plants and their microbial symbionts need to be co-propagated as life-long partners in future strategies for plant breeding. In this perspective, we propose bottom–up approach to co-propagate the co-evolved, the plant along with the target microbiome, through – (i) reciprocal soil transplantation method, or (ii) artificial ecosystem selection method of synthetic microbiome inocula, or (iii) by exploration of microRNA transfer method – for realizing this next-generation plant breeding approach. Our aim, thus, is to bring closer the information accrued through the advanced nucleotide sequencing and bioinformatics in conjunction with conventional culture-dependent isolation method for practical application in plant breeding and overall agriculture.

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Section: Conventional Selection Breeding Focused On Plant Genomementioning

confidence: 99%

Microbiome Selection Could Spur Next-Generation Plant Breeding Strategies

2016

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“…In general, very little is known about how rhizobia are co-introduced with legumes, but their free-living nature in soils, or presence within root nodules, suggests transport of seedlings in soil as a likely mechanism. On the other hand, compatible rhizobia may be found in novel regions as a result of human-mediated dispersal, such as for crop inocula (Ambrosini et al, 2016).…”

Section: Review 1355mentioning

confidence: 99%

Co‐introduction vs ecological fitting as pathways to the establishment of effective mutualisms during biological invasions

et al. 2017

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Contents 1354I.1354II.1355III.1357IV.1357V.13591359References1359 Summary Interactions between non‐native plants and their mutualists are often disrupted upon introduction to new environments. Using legume–rhizobium mutualistic interactions as an example, we discuss two pathways that can influence symbiotic associations in such situations: co‐introduction of coevolved rhizobia; and utilization of, and adaptation to, resident rhizobia, hereafter referred to as ‘ecological fitting’. Co‐introduction and ecological fitting have distinct implications for successful legume invasions and their impacts. Under ecological fitting, initial impacts may be less severe and will accrue over longer periods as novel symbiotic associations and/or adaptations may require fine‐tuning over time. Co‐introduction will have more profound impacts that will accrue more rapidly as a result of positive feedbacks between densities of non‐native rhizobia and their coevolved host plants, in turn enhancing competition between native and non‐native rhizobia. Co‐introduction can further impact invasion outcomes by the exchange of genetic material between native and non‐native rhizobia, potentially resulting in decreased fitness of native legumes. A better understanding of the roles of these two pathways in the invasion dynamics of non‐native legumes is much needed, and we highlight some of the exciting research avenues it presents.

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“…PGP assays and evaluation of beneficial characteristics provide data to select isolates based in their potential to be a “good inoculant.” Nevertheless, only assays for plant‐bacteria interaction in controlled‐environment chambers and in the field, in natural conditions, are conclusive regarding PGPB efficiency. Plant‐inoculant interactions are specific to plant and bacteria genotypes, varying with the geochemical characteristics of different types of soil and localities and with the biological interactions among the soil biota (Ambrosini, Souza, & Passaglia, ).…”

Section: Commentarymentioning

confidence: 99%

Plant Growth–Promoting Bacteria (PGPB): Isolation and Screening of PGP Activities

Ambrosini

Passaglia

2017

CP Plant Biology

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Plant roots are associated with numerous and diverse types of beneficial and pathogenic microorganisms. Plant growth–promoting (rhizo)bacteria (PGPB or PGPR) are isolated from plants crops worldwide, and many of them are used as agricultural inoculants. Agricultural biofertilization and biocontrol of pathogens are eco‐friendly alternatives to chemical usage and have less energy, environmental, and economic costs. PGPB isolation and evaluation are essentials steps for determining bacteria that are able to improve plant development and productivity. In this unit, we present protocols to isolate bacteria from soil and plant roots (“putative” diazotrophic and endospore‐forming bacteria), as well to evaluate some of their beneficial characteristics for the promotion of plant growth (e.g., nitrogen fixation, production of indolic compounds and siderophores, phosphate solubilization, and 1‐aminocyclopropane‐1‐carboxylate deaminase activity). © 2017 by John Wiley & Sons, Inc.

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Ecological role of bacterial inoculants and their potential impact on soil microbial diversity

Cited by 130 publications

References 136 publications

Microbiome Selection Could Spur Next-Generation Plant Breeding Strategies

Microbiome Selection Could Spur Next-Generation Plant Breeding Strategies

Co‐introduction vs ecological fitting as pathways to the establishment of effective mutualisms during biological invasions

Plant Growth–Promoting Bacteria (PGPB): Isolation and Screening of PGP Activities

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