Ecological, Hematological and Parasitological Studies on Oreochromis niloticus Linnaeus 1757 in the Nile Delta Region, Egypt

Omar, Rana H.; Hagras, Ahmed A.; El-Naggar, Ahmed M.; Mashaly, Mohamed I.

doi:10.21608/ejabf.2021.150883

Cited by 7 publications

(6 citation statements)

References 35 publications

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“…In this study, a significant increase in nuclear abnormalities in Aceta and Imid-exposed groups was observed. The loss of a chromosomal part or the whole during cell division usually leads to micronucleus (MN) formation which is considered as a genotoxicity indicator (Schmid 1975 ; Omar et al 2021 ). Clastogenic agents may cause these abnormalities by altering the integrity of DNA leading to chromosomal fragmentation or modifying mitosis and causing cell spindle breakdown as aneugenic agents (Abdel-Khalek et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Neonicotinoids Exposure on Oreochromis niloticus Histopathological Alterations and Genotoxicity

El-Garawani

Khallaf

Alne-na-ei

et al. 2022

Bull Environ Contam Toxicol

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This study aimed to examine the side effects of selected neonicotinoids (Acetamiprid, Aceta, and Imidacloprid, Imid) on Oreochromis niloticus juveniles. The acute toxicity, Probit method, revealed an LC50 of 195.81 and 150.76 ppm for Aceta/96 h and Imid/72 h respectively. The fish were divided into three groups that were exposed, for 21 days (n = 5/replicate), to 1/10 of the LC50 of either neonicotinoids, however, the third was an unexposed control group. Results of erythrocytic micronucleus (MN), and nuclear abnormalities (NA) showed that Aceta and Imid exposure caused a significant (p < 0.05) increase in MN by ~ 2.2 and ~ 10 folds, respectively relative to control. NAs occurred at the order of kidney-shaped > budding > binucleated in Aceta, however, budding > binucleated > kidney-shaped was noticed in the Imid group. Histopathological changes in gills, liver, and muscles were observed significantly in both exposed groups with more severity in the Imid group. Collectively, Aceta and Imid have potential genotoxicity and histopathological alterations in O. niloticus.

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Section: Discussionmentioning

confidence: 99%

The Effect of Neonicotinoids Exposure on Oreochromis niloticus Histopathological Alterations and Genotoxicity

El-Garawani

Khallaf

Alne-na-ei

et al. 2022

Bull Environ Contam Toxicol

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“…Institutional Animal Care and Use Committee (IACUC), Menoufia University, Egypt. The protocol of this study has been approved by the ethics review board of the IACUC of Faculty of Science (ID: MUFS-F-EC- [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. The experiments in this study were in compliance with the ARRIVE guidelines.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, tilapia became the most common freshwater fishes in aquaculture worldwide 3 , 4 . They are the most frequently farmed fish species in Egypt, accounting for 43.5% of farmed fish and 24% of total fisheries production 5 .…”

Section: Introductionmentioning

confidence: 99%

The role of ascorbic acid combined exposure on Imidacloprid-induced oxidative stress and genotoxicity in Nile tilapia

El-Garawani

Khallaf

Alne-na-ei

et al. 2021

Sci Rep

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Imidacloprid (Imid), a systemic neonicotinoid insecticide, is broadly used worldwide. It is reported to contaminate aquatic systems. This study was proposed to evaluate oxidative stress and genotoxicity of Imid on Nile tilapia (Oreochromis niloticus) and the protective effect of ascorbic acid (Asc). O. niloticus juveniles (30.4 ± 9.3 g, 11.9 ± 1.3 cm) were divided into six groups (n = 10/replicate). For 21 days, two groups were exposed to sub-lethal concentrations of Imid (8.75 ppm, 1/20 of 72 h-LC50 and 17.5 ppm, 1/10 of 72 h-LC50); other two groups were exposed to Asc (50 ppm) in combination with Imid (8.75 and 17.5 ppm); one group was exposed to Asc (50 ppm) in addition to a group of unexposed fish which served as controls. Oxidative stress was assessed in the liver where the level of enzymatic activities including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) in addition to mRNA transcripts and, Lipid peroxidation (LPO) were evaluated. Moreover, mitotic index (MI) and comet assay were performed, in addition, the erythrocytic micronucleus (MN), and nuclear abnormalities (NA) were observed to assess genotoxicity in fish. Imid exposure induced significant (p ˂ 0.05) changes in the antioxidant profile of the juveniles' liver by increasing the activities and gene expression of SOD, CAT and GPX as well as elevating the levels of LPO. DNA strand breaks in gill cells, erythrocytes and hepatocytes along with erythrocytic MN and NA were also significantly elevated in Imid-exposed groups. MI showed a significant (p ˂ 0.05) decrease associated with Imid exposure. Asc administration induced a significant amelioration towards the Imid toxicity (8.75 and 17.5 ppm). A significant protective potency against the genotoxic effects of Imid was evidenced in Asc co-treated groups. Collectively, results highlight the importance of Asc as a protective agent against Imid-induced oxidative stress and genotoxicity in O. niloticus juveniles.

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“…The utilization of blood parameters to unify physiological and pathological changes in fish is both effective and highly sensitive. Blood serves as a vital diagnostic tool for assessing health, as it exhibits varying levels of fish blood response and is influenced by diverse stress conditions [42].…”

Section: Relationship Between Levels Of Water Quality and Blood Profilementioning

confidence: 99%

Blood and Micronuclei Analysis of Tawes Fish (Barbonymus gonionotus) Correlation with Water Quality in the Boon Pring Ecotourism Area, Turen, Malang Regency

Hertika,

Risjani,

Buwono

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Tawes (Barbonymus gonionotus) is a type of endemic fish that lives in the Boon Pring Reservoir, Turen, and Malang Regency. This study aims to analyze the water quality and blood profile of Tawes Fish (Barbonymus gonionotus) in Boon Pring Ecotourism and to determine the relationship between water quality and fish health through their blood profile using the Canonical Correspondence Analysis (CCA) method. The results obtained after measuring the water quality during two samplings at both locations were that the temperature ranged from 21.7–27C, TDS 111–145 mg/l, TSS 2-4 mg/l, pH 6-6.7, DO 8–10.1 mg/l, BOD 0.44-0.98 mg/l, and ammonia 0.026–0.075 mg/l. Both locations are classified as having good or normal conditions. Observations on the blood profile of Tawes fish (Barbonymus gonionotus) are healthy and normal. The average number of erythrocytes was 2,586,667 cells/mm3, leukocytes were 60,500 cells/mm3, lymphocytes were 76.8%, monocytes were 1.4%, neutrophils were 3.3%, hemoglobin was 9.8 g/mL, hematocrit was 34.6%, and micronuclei were 12.3 cells/1000. The results of data analysis using CCA showed that all blood and micronuclei profiles of fish were affected by water quality parameters, both significantly and moderately to severely.

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Ecological, Hematological and Parasitological Studies on Oreochromis niloticus Linnaeus 1757 in the Nile Delta Region, Egypt

Cited by 7 publications

References 35 publications

The Effect of Neonicotinoids Exposure on Oreochromis niloticus Histopathological Alterations and Genotoxicity

The Effect of Neonicotinoids Exposure on Oreochromis niloticus Histopathological Alterations and Genotoxicity

The role of ascorbic acid combined exposure on Imidacloprid-induced oxidative stress and genotoxicity in Nile tilapia

Blood and Micronuclei Analysis of Tawes Fish (Barbonymus gonionotus) Correlation with Water Quality in the Boon Pring Ecotourism Area, Turen, Malang Regency

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