The role of ascorbic acid combined exposure on Imidacloprid-induced oxidative stress and genotoxicity in Nile tilapia

El-Garawani, Islam; Khallaf, Elsayed A.; Alne-na-ei, Alaa A.; Elgendy, Rehab G.; Mersal, Gaber A.M.; El‐Seedi, Hesham R.

doi:10.1038/s41598-021-94020-y

Cited by 20 publications

(9 citation statements)

References 77 publications

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“…For instance, paraquat (1,1′-dimethyl-4,4′-bipyridinium dichloride), a non-selective herbicide, at non-cytotoxic concentrations, induces oxidative DNA damage in HepG2 cells [ 12 ]. Imidacloprid, a neonicotinoid insecticide, induces oxidative stress and DNA damage on non-target organisms, namely various fish species such as Danio rerio (zebrafish) [ 13 ], Prochilodus lineatus [ 14 ], and Oreochromis niloticus (Nile tilapia) [ 15 ], affecting various organs (e.g., gills, kidney, brain, liver), as assessed by the levels of stress defence enzyme activity and by comet assay [ 13 , 14 , 15 ]. Imidacloprid was also reported to induce oxidative stress and inflammation in the hepatic and central nervous system of rats [ 16 ] and was associated with multiple neurobehavioral aberrations in adolescent and adult rats [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Physicochemical Properties of Selected Pesticides as Predictive Factors for Oxidative Stress and Apoptosis-Dependent Cell Death in Caco-2 and HepG2 Cells

Silva

Martins-Gomes

Ferreira

et al. 2022

IJMS

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In this work, three pesticides of different physicochemical properties: glyphosate (GLY, herbicide), imidacloprid (IMD, insecticide), and imazalil (IMZ, fungicide), were selected to assess their cytotoxicity against Caco-2 and HepG2 cells. Cell viability was assessed by the Alamar Blue assay, after 24 and 48 h exposure to different concentrations, and IC50 values were calculated. The mechanisms underlying toxicity, namely cellular reactive oxygen species (ROS), glutathione (GSH) content, lipid peroxidation, loss of mitochondrial membrane potential (MMP), and apoptosis/necrosis induction were assessed by flow cytometry. Cytotoxic profiles were further correlated with the molecular physicochemical parameters of pesticides, namely: water solubility, partition coefficient in an n-octanol/water (Log Pow) system, topological polar surface area (TPSA), the number of hydrogen-bonds (donor/acceptor), and rotatable bonds. In vitro outputs resulted in the following toxicity level: IMZ (Caco-2: IC50 = 253.5 ± 3.37 mM, and HepG2: IC50 = 94 ± 12 mM) > IMD (Caco-2: IC50 > 1 mM and HepG2: IC50 = 624 ± 24 mM) > GLY (IC50 > >1 mM, both cell lines), after 24 h treatment, being toxicity time-dependent (lower IC50 values at 48 h). Toxicity is explained by oxidative stress, as IMZ induced a higher intracellular ROS increase and lipid peroxidation, followed by IMD, while GLY did not change these markers. However, the three pesticides induced loss of MMP in HepG2 cells while in Caco-2 cells only IMZ produced significant MMP loss. Increased ROS and loss of MMP promoted apoptosis in Caco-2 cells subjected to IMZ, and in HepG2 cells exposed to IMD and IMZ, as assessed by Annexin-V/PI. The toxicity profile of pesticides is directly correlated with their Log Pow, as affinity for the lipophilic environment favours interaction with cell membranes governs, and is inversely correlated with their TPSA; however, membrane permeation is favoured by lower TPSA. IMZ presents the best molecular properties for membrane interaction and cell permeation, i.e., higher Log Pow, lower TPSA and lower hydrogen-bond (H-bond) donor/acceptor correlating with its higher toxicity. In conclusion, molecular physicochemical factors such as Log Pow, TPSA, and H-bond are likely to be directly correlated with pesticide-induced toxicity, thus they are key factors to potentially predict the toxicity of other compounds.

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Section: Introductionmentioning

confidence: 99%

Molecular Physicochemical Properties of Selected Pesticides as Predictive Factors for Oxidative Stress and Apoptosis-Dependent Cell Death in Caco-2 and HepG2 Cells

Silva

Martins-Gomes

Ferreira

et al. 2022

IJMS

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“…Although the MN frequency in the erythrocytes of P. lineatus did not increase significantly, the ENAs frequency increased significantly as a result of aluminum exposure (Galindo et al, 2010). However, as in the erythrocytes of C. carpio individuals exposed to metal(oid) mixtures, it was reported that the frequency of both MN and ENA was also increased in erythrocyte cells of Nil tilapia exposed to ascorbic acid (ElGarawani et al, 2021). The different types of ENAs were determined such as blebbed nuclei (B), binucleated cell (BN), kidney-shaped nuclei (KS), lobed nuclei (L), and notched nuclei (N) in the erythrocyte cells of C. carpio in the present study.…”

Section: Discussionmentioning

confidence: 99%

In vivo assessment of genotoxic effects in Cyprinus carpio L., 1758 (Teleostei: Cyprinidae) exposed to selected metal(oid)s

Kontaş

2022

Toxicol Ind Health

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Heavy metals and metalloids originating from industrial, agricultural, and urban wastes and increasing in aquatic ecosystems cause genotoxic damage to fish species. This study aimed to determine the potential genotoxic effects of mixtures of aluminum, arsenic, and manganese in Cyprinus carpio. The effects of the mixtures on erythrocyte cells of C. carpio were examined using the comet assay, micronucleus test, and erythrocytic nuclear abnormalities in two groups after exposure to three doses of the mixtures (Group A; Dose 1: 0.3 + 0.1 + 0.02 mg/L, Dose 2: 0.6 + 0.2 + 0.04 mg/L, Dose 3: 0.9 + 0.3 + 0.06 mg/L and Group B; Dose 1: 1 + 3 +0.1 mg/L, Dose 2: 2 + 6 + 0.2 mg/L, Dose 3: 3 + 9 + 0.3 mg/L). Experimental groups were formed according to the permissible limits specified in the Turkish Surface Water Quality Regulation (TSWQR). The results of comet assay parameters such as tail DNA %, tail moment, and olive tail moment confirmed the genotoxic effect of metal(oid)s mixtures on erythrocyte cells compared with control groups and showed that DNA damage increased with increasing the concentrations. The micronucleus and other nuclear abnormalities such as blebbed nuclei, notched nuclei, eightshaped nuclei, lobed nuclei, and binucleated cells were detected in the erythrocyte cells exposed to the mixtures. Consequently, it was found that the frequency of micronucleus and erythrocytic nuclear abnormalities significantly increased in the erythrocyte cells exposed to metal(oid) concentrations compared to control groups. These results show the existence of potential genotoxicity in C. carpio even at the minimum values specified in the TSWQR after exposure to the mixtures.

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“…In the literature, studies confirming the harmful effects on fish of IMI and THI, two NEO insecticides frequently detected in aquatic environments, involve pure parent compounds or pesticide formulations, not their metabolites. It is known that by generating DNA damage and inducing oxidative stress, they damage tissues of Danio rerio , Oreochromis niloticus , Prochilodus lineatus 22 , 23 , 27 , 30 – 33 .…”

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of neonicotinoids and their metabolites-induced oxidative stress in carp primary leukocytes and CLC cells

Rymuszka,

Sieroslawska

2024

Sci Rep

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Neonicotinoids (NEOs) have been designed to act selectively on insect nicotinic acetylcholine receptors (nAChRs). However, nAChRs are also expressed in vertebrate immune cells, so NEOs may interfere with the immune system in exposed non-target animals. The present study shows that NEOs: imidacloprid and thiacloprid, and their main metabolites: desnitro-imidacloprid and thiacloprid amide, at sub-micromolar concentrations ranging from 2.25 to 20 μM, affect the immune cells of fish. This was found both in primary cultures of leukocytes isolated from the carp head kidney and in the continuous adherent carp monocyte/macrophage cell line. Moreover, the results revealed that the studied pesticides and metabolites generate oxidative stress in carp immune cells and that this is one of the most important mechanisms of neonicotinoid immunotoxicity. Significant increases were observed in the formation of ROS and malondialdehyde (MDA). The antioxidant status alteration was linked with decrease in antioxidant enzyme activity: superoxide dismutase (SOD), catalase (CAT), and non-enzymatic antioxidant glutathione (GSH). Importantly, the metabolites: desnitro-imidacloprid and thiacloprid amide showed significantly higher cytotoxicity towards fish leukocytes than their parent compounds, imidacloprid and thiacloprid, which emphasizes the importance of including intermediate metabolites in toxicology studies.

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The role of ascorbic acid combined exposure on Imidacloprid-induced oxidative stress and genotoxicity in Nile tilapia

Cited by 20 publications

References 77 publications

Molecular Physicochemical Properties of Selected Pesticides as Predictive Factors for Oxidative Stress and Apoptosis-Dependent Cell Death in Caco-2 and HepG2 Cells

Molecular Physicochemical Properties of Selected Pesticides as Predictive Factors for Oxidative Stress and Apoptosis-Dependent Cell Death in Caco-2 and HepG2 Cells

In vivo assessment of genotoxic effects in Cyprinus carpio L., 1758 (Teleostei: Cyprinidae) exposed to selected metal(oid)s

Comparative evaluation of neonicotinoids and their metabolites-induced oxidative stress in carp primary leukocytes and CLC cells

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