Ecological characterisation of the Colombian entomopathogenic nematode Heterorhabditis sp. SL0708

Mejia-Torres, MC; Sáenz, Adriana

doi:10.1590/s1519-69842013000200003

Cited by 10 publications

(7 citation statements)

References 22 publications

(29 reference statements)

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“…The hermaphroditic individual lays some of its eggs in the host's body cavity and retains the remainder of the eggs inside its uterus. This phenomenon is confirmed by observations of Boff et al (2001) and Wang and Bedding (1996) who analyse the growth cycle of H. bacteriophora. Wang and Bedding (1996) observed the life cycle of H. bacteriophora using an initial dose of 1 IJ.…”

Section: Discussionsupporting

confidence: 80%

The effect of initial dose on the recovery and final yields of Heterorhabditis megidis (Rhabditida: Heterorhabditidae) in larvae of the great wax moth, Galleria mellonella

Tumialis

Pezowicz

Mazurkiewicz

et al. 2014

Acta Parasitologica

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The aim of this study was to determine the effect of different initial doses of the infective juveniles (IJs) (50 IJs, 200 IJs, 1000 IJs ) of Heterorhabditis megidis Poinar (Rhabditida: Heterorhabditidae) strain IsM15/09 on recovery, final yields and percent final yields in larvae Galleria mellonella ( L.). Percent recovery was not directly related to initial dose. Final yields also did not change with the initial dose. However, percent yields was highly negatively correlated with initial dose of nematodes and was the highest with the 50 IJs dose. Additional point of the study was to investigate whether the nematodes are able to produce progeny from one hermaphroditic individual. The results showed that the invasive larvae resumed growth and transformed into hermaphroditic individuals that reproduced without cross-fertilisation.

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Section: Discussionsupporting

confidence: 80%

The effect of initial dose on the recovery and final yields of Heterorhabditis megidis (Rhabditida: Heterorhabditidae) in larvae of the great wax moth, Galleria mellonella

Tumialis

Pezowicz

Mazurkiewicz

et al. 2014

Acta Parasitologica

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“…This indicates that these isolates cannot persist in soil with low moisture level. The low potential of Heterorhabditis to survive low moisture level and desiccation has been discussed by Grewal et al (2006) , Mukuka et al (2010) , and Mejia-Torres and Saenz (2013) . However, the result of this study is contrary to the results reported for H. indica strain T2 (geographical origin: Thailand) which has been reported to infect G. mellonella larvae at moisture level as low as 3% ( Maketon et al, 2011 ), whereas Fijian isolates of H. indica were unable to cause any mortality at 5% moisture level.…”

Section: Discussionmentioning

confidence: 99%

“…In current study, even though the optimum concentration was 1000 IJ/ml for the storage experiment, poor storage stability was observed for native isolates of H. indica which is slightly higher when compared to Colombian Heterorhabditis sp. SL0708 (survive only up to 8 weeks) ( Mejia-Torres and Saenz, 2013 ). Low longevity in H. indica population has also been reported by Shapiro-Ilan et al (2006) .…”

Section: Discussionmentioning

confidence: 99%

Ecological Characterisation of Native Isolates of Heterorhabditis indica from Viti Levu, Fiji Islands

Kour

Khurma

Brodie

2021

Journal of Nematology

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Entomopathogenic nematodes (EPNs) in the families Steinernematidae and Heterorhabditidae are obligate parasites of soil inhibiting insects. EPNs are being widely researched as promising biocontrol agents for a wide range of agricultural pests. It is known that strains of EPNs isolated from different geographical regions differ in their attributes, such as host-finding ability, host range, infectivity, reproduction, and environmental stress tolerance. A precise knowledge of these factors is therefore an essential pre-requisite for devising successful strategies to use these nematodes in biological control programmes. Thus, ecological characterisation of the EPN Heterorhabditis indica (Rhabditida: Heterorhabditidae) newly isolated and representing the only species of EPN reported from the island of Viti Levu, Fiji was carried out using Galleria mellonella larvae (L) (Pyralidae: Galleriinae) as hosts to allow comparisons between bioassays conducted in different laboratories around the world. Temperature data showed that native isolates of H. indica are warm-adapted nematodes with thermal range for infectivity between 15˚C and 35˚C and can reproduce between 20˚C and 30˚C. They are highly virulent with LC50 values against G. mellonella ranging from 2.8 IJ to 3.8 IJ/larva. However, they showed poor desiccation tolerance and fail to infect hosts in soil with moisture levels below 8%. They showed a moderate level of hypoxic tolerance and can be stored at 15˚C for 4 months. Results also showed great variability within the selected native isolates of H. indica. Beneficial traits for selected isolates were added up to identify a superior candidate. The current study also suggested that the thermal niche breadth for infection can differ among conspecific strains of an EPN species. The results of this experimental study on ecological aspects of these native isolates of H. indica should form a basis for their potential use in biological control of insect pests in Fiji.

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“…Temperatures between 16-10 °C as observed in Porto Amazonas during winter usually reduces EPN efficacy (Klein, 1990;Mejia-Torres and Sáenz, 2013). However, winter temperatures seemed to be high enough to assure nematode capture during the entire year, even because samples were kept at 25 ºC in the laboratory, what favored the infection.…”

Section: Discussionmentioning

confidence: 99%

Isolation of entomopathogenic nematodes in an apple orchard in Southern Brazil and its virulence to Anastrepha fraterculus (Diptera: Tephritidae) larvae, under laboratory conditions

et al. 2017

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Entomopathogenic nematodes (EPNs) are a promising alternative to integrated control in many fruit pests. Few studies were made on the relationship of Anastrepha fraterculus natural population with native EPNs population and other biotic and abiotic factors. The aim of this work was to verify the occurrence of endemic nematodes in an apple orchard, concerning environmental conditions and technical procedure, and access isolates virulence to A. fraterculus larvae. The experiment was conducted during a year taking monthly soil samples from an apple orchard, with and without fallen fruits just above the soil. Samples were baited with Tenebrium molitor and A. fraterculus larvae in laboratory. Canopy and fallen fruits were sampled to access the pest infestation. Seventy three EPN isolates were captured, in 23.2% soil samples, more with T. molitor than with A. fraterculus baits. From the 20 isolates tested against A. fraterculus, only five were pathogenic, and they were identified as Oscheius sp. The nematodes were captured during all seasons in a similar frequency. Soil and weather conditions, presence of fruit over the orchard soil, and A. fraterculus pupae in the fruits had no significant influence on the capture. As a conclusion, nematodes of the genera Oscheius are found in an apple orchard of Porto Amazonas constantly along the year, independently of fluctuations in A. fraterculus population, climate conditions and presence of fruit over the soil. Some of the isolates are pathogenic to A. fraterculus. Keywords

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Ecological characterisation of the Colombian entomopathogenic nematode Heterorhabditis sp. SL0708

Cited by 10 publications

References 22 publications

The effect of initial dose on the recovery and final yields of Heterorhabditis megidis (Rhabditida: Heterorhabditidae) in larvae of the great wax moth, Galleria mellonella

The effect of initial dose on the recovery and final yields of Heterorhabditis megidis (Rhabditida: Heterorhabditidae) in larvae of the great wax moth, Galleria mellonella

Ecological Characterisation of Native Isolates of Heterorhabditis indica from Viti Levu, Fiji Islands

Isolation of entomopathogenic nematodes in an apple orchard in Southern Brazil and its virulence to Anastrepha fraterculus (Diptera: Tephritidae) larvae, under laboratory conditions

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