SummaryThis study reports the first record of Steinernema kraussei from Poland. The nematode was isolated from coniferous woodlands in 4 localities in central Poland. Preliminary identification of the species was done based on morphometric measurements. To confirm nematode species of the genus Steinernema the result was supported by the description of the ITS region.
Small banded pine weevil Pissodes castaneus is one of the most dangerous pests of Pinus sylvestris plantations and thickets. The lack of effective and environmentally safe methods of limiting the number of the pest justified to undertake the studies aimed at the laboratory and field evaluation of biological activity of entomopathogenic fungus Beauveria bassiana used to reduce the numbers of small banded pine weevil.In laboratory, the beetles were reared on the sections of fresh Scots pine twigs that were treated with five suspensions containing from 1 × 10 4 to 1 × 10 8 conidia of B. bassiana in 1 ml. During the 3-week rearing, insect mortality was determined and median lethal concentration LC 50 was calculated. The field treatments consisted of spraying 4-year-old P. sylvestris trees with two formulations of fungus containing 1 × 10 8 conidia ml −1 of suspension. Treatments consisted of spraying 4-year-old P. sylvestris trees with two fungus formulations containing 1 × 10 8 conidia ml −1 . High insecticidal activity of B. bassiana was found because the pathogen caused the death of 14-94% of P. castaneus beetles, LC 50 = 6.51 × 10 5 conidia ml −1. Field treatments did not result in the reduction of plant damage caused by small banded pine weevil; therefore, the spraying of trees with B. bassiana cannot be recommended to protect the young stands of P. sylvestris against pest.
The aim of this study was to determine the effect of different initial doses of the infective juveniles (IJs) (50 IJs, 200 IJs, 1000 IJs ) of Heterorhabditis megidis Poinar (Rhabditida: Heterorhabditidae) strain IsM15/09 on recovery, final yields and percent final yields in larvae Galleria mellonella ( L.). Percent recovery was not directly related to initial dose. Final yields also did not change with the initial dose. However, percent yields was highly negatively correlated with initial dose of nematodes and was the highest with the 50 IJs dose. Additional point of the study was to investigate whether the nematodes are able to produce progeny from one hermaphroditic individual. The results showed that the invasive larvae resumed growth and transformed into hermaphroditic individuals that reproduced without cross-fertilisation.
Sensitivity of Pieris brassicae, P. napi and P. rapae (Lepidoptera: Pieridae) larvae to native strains of Steinernema feltiae (Filipjev, 1934) Anna Mazurkiewicz Abstract The study was aimed at determining the sensitivity of three species of Pieris sp. to native strains of Steinernema feltiae. Studied strains were highly pathogenic to all Pieris species (the extensity varied from 80 to 100%).
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