Ecoenzyme activity ratios reveal interactive effects of nutrient inputs and UVR in a Mediterranean high-mountain lake

Ayuso, Sergio Velasco; Medina‐Sánchez, Juan Manuel; Guénon, René; Carrillo, Presentación

doi:10.1007/s10533-016-0288-3

Cited by 6 publications

(4 citation statements)

References 65 publications

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“…Given the link between the microbial metabolism and environmental energy/resource availability provided by the ratios between EA ( Sinsabaugh et al, 2009 ; Sinsabaugh and Follstad-Shah, 2012 ), the calculated ratios in our study (GLU:AP < 1; GLU:UR > 1; GLU:AS ∼ 1, UR:AP < 1; AS:UR > 1) indicate a resource limitation ordered as P > C = S > N, an order which was even intensified over the ice-free period ( Figure 1 ). This is consistent with the patterns of resource limitation found in La Caldera under events of Saharan dust-aerosol deposition ( Velasco-Ayuso et al, 2017 ).…”

Section: Discussionsupporting

confidence: 90%

“…EA ratios (GLU:AP, GLU:UR, GLU:AS, UR:AP, UR:AS) were calculated as indicators of nutrient-ratio limitations (C:P, C:N, C:S, N;P, N:S, respectively) according to previous studies on EA ( Sinsabaugh et al, 2009 ; Sinsabaugh and Follstad-Shah, 2012 ; Velasco-Ayuso et al, 2017 ). Propagation errors were used to calculate the variance of each EA ratio.…”

Section: Methodsmentioning

confidence: 99%

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Denitrification and Biodiversity of Denitrifiers in a High-Mountain Mediterranean Lake

et al. 2017

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Wet deposition of reactive nitrogen (Nr) species is considered a main factor contributing to N inputs, of which nitrate (NO3−) is usually the major component in high-mountain lakes. The microbial group of denitrifiers are largely responsible for reduction of nitrate to molecular dinitrogen (N2) in terrestrial and aquatic ecosystems, but the role of denitrification in removal of contaminant nitrates in high-mountain lakes is not well understood. We have used the oligotrophic, high-altitude La Caldera lake in the Sierra Nevada range (Spain) as a model to study the role of denitrification in nitrate removal. Dissolved inorganic Nr concentration in the water column of la Caldera, mainly nitrate, decreased over the ice-free season which was not associated with growth of microbial plankton or variations in the ultraviolet radiation. Denitrification activity, estimated as nitrous oxide (N2O) production, was measured in the water column and in sediments of the lake, and had maximal values in the month of August. Relative abundance of denitrifying bacteria in sediments was studied by quantitative polymerase chain reaction of the 16S rRNA and the two phylogenetically distinct clades nosZI and nosZII genes encoding nitrous oxide reductases. Diversity of denitrifiers in sediments was assessed using a culture-dependent approach and after the construction of clone libraries employing the nosZI gene as a molecular marker. In addition to genera Polymorphum, Paracoccus, Azospirillum, Pseudomonas, Hyphomicrobium, Thauera, and Methylophaga, which were present in the clone libraries, Arthrobacter, Burkholderia, and Rhizobium were also detected in culture media that were not found in the clone libraries. Analysis of biological activities involved in the C, N, P, and S cycles from sediments revealed that nitrate was not a limiting nutrient in the lake, allowed N2O production and determined denitrifiers’ community structure. All these results indicate that denitrification could be a major biochemical process responsible for the N losses that occur in La Caldera lake.

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Section: Discussionsupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Denitrification and Biodiversity of Denitrifiers in a High-Mountain Mediterranean Lake

et al. 2017

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“…The range of limitations observed in lakes is unsurprising given the range of lentic ecosystems EE potentials were drawn from and highlights the need for future research to understand how nutrient limitations are connected across freshwater ecosystems from headwater streams and alpine lakes to major rivers and lakes in low‐elevation basins. In alpine watersheds, studies suggest that C is limiting (Bigelow et al, 2019; Velasco Ayuso et al, 2017), so microbial activity in snow may provide essential bioavailable C. Additionally, long‐term atmospheric N deposition has led to P limitation in freshwater ecosystems, but recent declines in N deposition have shifted some areas back to N and P co‐limitation (Bergström et al, 2020). However, P deposition in dust and from forest fires is also increasing, and P inputs may have a greater impact on ecosystems because of the nutrient requirements by organisms (Brahney et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…We report mean enzyme potentials from 6 studies for 16 different lentic ecosystems (Hoostal et al, 2008; Jackson et al, 2013; Kalwasińska & Brzezinska, 2013; Munster et al, 1992; Song et al, 2018; Velasco Ayuso et al, 2017) and from five studies for 11 different lotic ecosystems (Bullock et al, 2017; Harjung et al, 2019; Hendel & Marxsen, 1997; Sinsabaugh et al, 1997; Sinsabaugh & Foreman, 2001). The only methodological difference between studies was that some studies used a NaOH addition after incubation and before measurement, which increases detection of fluorescent products at low levels, but was not necessary for our measurements.…”

Section: Methodsmentioning

confidence: 99%

Potential microbial enzyme activity in seasonal snowpack is high and reveals P limitation

et al. 2022

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Microbes in snow and ice ecosystems in polar regions contribute substantially to C, N, and P cycling, but few studies have explored microbial activity in seasonal snow. The purpose of this study was to explore the relative importance of snow microbial processing of C, N, and P compounds in atmospheric deposition and litter and detect elemental limitations of snow microbes in Rocky Mountain conifer forests. Enzyme activity in snow was orders of magnitude greater than activity reported for lentic and lotic waters in similar environments. Proportions of C/P-and C/N-acquiring enzymes suggest that snow samples were P limited, or C and P co-limited, while lentic and lotic waters were more N limited. As such, microbes in seasonal snow may change the composition of nutrients and carbon, but these processes are vulnerable to changes in atmospheric deposition and snow extent and duration, which could affect nutrient processing across large areas.

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High Mountain Lakes as Remote Sensors of Global Change

Medina‐Sánchez

Cabrerizo

González-Olalla

et al. 2022

The Landscape of the Sierra Nevada

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Ecoenzyme activity ratios reveal interactive effects of nutrient inputs and UVR in a Mediterranean high-mountain lake

Cited by 6 publications

References 65 publications

Denitrification and Biodiversity of Denitrifiers in a High-Mountain Mediterranean Lake

Denitrification and Biodiversity of Denitrifiers in a High-Mountain Mediterranean Lake

Potential microbial enzyme activity in seasonal snowpack is high and reveals P limitation

High Mountain Lakes as Remote Sensors of Global Change

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