Eco-friendly approach for control of fungal deterioration of archaeological skeleton dated back to the Greco-Roman period

Abdel-Maksoud, Gomaa; Gaballah, Samiaa; Youssef, Ahmed M.; Eid, Ahmed M.; Sultan, Mahmoud H.; Fouda, Amr

doi:10.1016/j.culher.2022.11.005

Cited by 9 publications

(6 citation statements)

References 55 publications

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“…The production of toxic ions which in the current study are Cu 2+ after the breakdown of NPs inside the bacterial cells is considered the main mechanism for the inhibitory action of CuO-NPs. These liberated toxic ions react with the thiol group of protein and hence inhibit their function [ 86 ]. Also, the accumulation of these ions can be destroying the selective permeability function of the cytoplasmic membrane [ 87 ].…”

Section: Resultsmentioning

confidence: 99%

Myco-synthesized copper oxide nanoparticles using harnessing metabolites of endophytic fungal strain Aspergillus terreus: an insight into antibacterial, anti-Candida, biocompatibility, anticancer, and antioxidant activities

Nassar¹,

Atta²,

Abdel-Rahman³

et al. 2023

BMC Complement Med Ther

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Background The overuse of antibiotics leads to the emergence of antibiotic-resistant microbes which causes high mortality worldwide. Therefore, the synthesis of new active compounds has multifunctional activities are the main challenge. Nanotechnology provides a solution for this issue. Method The endophytic fungal strain Aspergillus terreus BR.1 was isolated from the healthy root of Allium sativum and identified using internal transcribed spacer (ITS) sequence analysis. The copper oxide nanoparticles (CuO-NPs) were synthesized by harnessing the metabolites of the endophytic fungal strain. The UV-Visble spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), Transmission electron micrscopy (TEM), Energy dispersive X-ray (EDX), X-ray diffraction (XRD), Dynamic light scattering (DLS), and zeta potential (ζ) were used for the characterization of synthesized CuO-NPs. The activity against different pathogenic bacteria and Candida species were investigated by agar well-diffusion method. The biocombatibility and anticancer activity were assessed by MTT assay method. The scavenging of DPPH was used to investigate the antioxidant activity of synthesized CuO-NPs. Results Data showed the successful formation of crystalline nature and spherical shape CuO-NPs with sizes in the ranges of 15–55 nm. The EDX reveals that the as-formed sample contains ions of C, O, Cl, and Cu with weight percentages of 18.7, 23.82, 11.31, and 46.17%, respectively. The DLS and ζ-potential showed high homogeneity and high stability of synthesized CuO-NPs with a polydispersity index (PDI) of 0.362 and ζ-value of − 26.6 mV. The synthesized CuO-NPs exhibited promising antibacterial and anti-Candida activity (concentration-dependent) with minimum inhibitory concentration (MIC) values in the ranges of 25–50 µg mL–1. Moreover, the fungal mediated-CuO-NPs targeted cancer cells of MCF7 and PC3 at low IC50 concentrations of 159.2 ± 4.5 and 116.2 ± 3.6 µg mL–1, respectively as compared to normal cells (Vero and Wi38 with IC50 value of 220.6 ± 3.7 and 229.5 ± 2.1 µg mL–1, respectively). The biosynthesized CuO-NPs showed antioxidant activity as detected by the DPPH method with scavenging percentages of 80.5 ± 1.2% at a concentration of 1000 µg mL–1 and decreased to 20.4 ± 4.2% at 1.9 µg mL–1 as compared to ascorbic acid (control) with scavenging activity of 97.3 ± 0.2 and 37.5 ± 1.3% at the same concentrations, respectively. Conclusion The fungal mediated-CuO-NPs exhibited promising activity and can be integrated into various biomedical and theraputic applications.

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Section: Resultsmentioning

confidence: 99%

Myco-synthesized copper oxide nanoparticles using harnessing metabolites of endophytic fungal strain Aspergillus terreus: an insight into antibacterial, anti-Candida, biocompatibility, anticancer, and antioxidant activities

Nassar¹,

Atta²,

Abdel-Rahman³

et al. 2023

BMC Complement Med Ther

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“…Most of these studies used MNPs that were synthesized either via chemical, physical or a combination of both methods. Four studies [ 260 , 261 , 272 , 274 ] used biosynthesized MPs.…”

Section: Potential Use Of Metal Nanoparticles In Cultural Heritage Co...mentioning

confidence: 99%

“…The authors reported the ability of CaZn 2 (OH) 6 NPs to inhibit microbial growth and, despite substantial variability, their results revealed fungicidal effects on all microorganisms tested, with minimal fungicidal concentrations ranging between 156 and 1250 mg/mL. Abdel-Maksoud et al [ 274 ] studied the in vitro antimicrobial potential of AgNPs and MgONPs biosynthesized using Aspergillus niger and Rhizopus oryzae , respectively, against fungi isolated from an archaeological skeleton from the Greco-Roman period ( Aspergillus flavus , Aspergillus delicatus , Aspergillus parasiticus , Aspergillus niger , Aspergillus oryzae , Penicillium expansum , Penicillium oxalicum , Penicillium digitatum , Cladosporium sp. and Paecilomyces sp.).…”

Section: Potential Use Of Metal Nanoparticles In Cultural Heritage Co...mentioning

confidence: 99%

“…These differences might possibly be explained by distinct interaction of the MNPs with the outer membrane of the cells and their ability to release M x [ 275 ]. Additionally, the size of the MNPs seems to be inversely correlated with their antimicrobial potential, with smaller MNPs apparently being more effective than larger ones [ 274 ]. Overall, these studies show promising results regarding the antimicrobial potential of MNPs against cultural heritage microbial contaminants, which are further supported by the results presented in Section 5.1 .…”

Section: Potential Use Of Metal Nanoparticles In Cultural Heritage Co...mentioning

confidence: 99%

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Biosynthesis of Metal and Metal Oxide Nanoparticles Using Microbial Cultures: Mechanisms, Antimicrobial Activity and Applications to Cultural Heritage

et al. 2023

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Nanoparticles (1 to 100 nm) have unique physical and chemical properties, which makes them suitable for application in a vast range of scientific and technological fields. In particular, metal nanoparticle (MNPs) research has been showing promising antimicrobial activities, paving the way for new applications. However, despite some research into their antimicrobial potential, the antimicrobial mechanisms are still not well determined. Nanoparticles’ biosynthesis, using plant extracts or microorganisms, has shown promising results as green alternatives to chemical synthesis; however, the knowledge regarding the mechanisms behind it is neither abundant nor consensual. In this review, findings from studies on the antimicrobial and biosynthesis mechanisms of MNPs were compiled and evidence-based mechanisms proposed. The first revealed the importance of enzymatic disturbance by internalized metal ions, while the second illustrated the role of reducing and negatively charged molecules. Additionally, the main results from recent studies (2018–2022) on the biosynthesis of MNPs using microorganisms were summarized and analyzed, evidencing a prevalence of research on silver nanoparticles synthesized using bacteria aiming toward testing their antimicrobial potential. Finally, a synopsis of studies on MNPs applied to cultural heritage materials showed potential for their future use in preservation.

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“…Among the potential advantages of metallic nanoparticles are their antifungal properties. For example, it was recently demonstrated that metallic nanoparticles could inhibit the growth of fungi that cause the biodeterioration of archaeological materials [5] or mycotoxin producers [6]. The adverse effects of different fungal strains are not only related to biodeterioration, but also to their negative impacts on human and animal health or on agriculture.…”

Section: Introductionmentioning

confidence: 99%

Biogenic Silver and Copper Nanoparticles: Potential Antifungal Agents in Rice and Wheat Crops

Sanguiñedo,

Faccio,

Abreo

et al. 2023

Chemistry

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Metal nanoparticles are widely studied due to their various applications, such as their potential use in the control of phytopathogens and the promotion of plant growth, with a significant impact on agriculture. Various microbial metabolites are used to reduce and stabilize metals and metal oxides to the nanoscale. In the present work, the biological synthesis of silver and copper oxide nanoparticles using Trichoderma harzianum TA2 is reported. The nanoparticles were purified and characterized with complementary methodologies to obtain information on the size, distribution, morphology, surface charge, and functional groups of the nanoparticles. The in vitro antifungal activity of the nanoparticles against pathogens of rice and wheat, as well as their effect on seed germination, were evaluated. In general, the nanoparticles showed a spherical shape, an average size of 17–26 nm, and low polydispersity. Furthermore, they showed antifungal activity at low concentrations against Sclerotium oryzae (0.140 ηM), Rhizoctonia oryzae-sativae (0.140 ηM), Fusarium graminearum (0.034 ηM), and Pyricularia oryzae (0.034 ηM). The germination of seeds treated with nanoparticles was not negatively affected. This is the first report of biogenic silver and copper oxide nanoparticles from a single strain of T.harzianum with antifungal activity against four phytopathogens of interest in Uruguay. Furthermore, the synthesis of the biogenic nanoparticles was faster and more efficient than previous reports using other fungi. In conclusion, this work reveals that biogenic metallic nanoparticles from T. harzianum TA2 can be considered as candidates for the control of phytopathogens affecting important crops.

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Eco-friendly approach for control of fungal deterioration of archaeological skeleton dated back to the Greco-Roman period

Cited by 9 publications

References 55 publications

Myco-synthesized copper oxide nanoparticles using harnessing metabolites of endophytic fungal strain Aspergillus terreus: an insight into antibacterial, anti-Candida, biocompatibility, anticancer, and antioxidant activities

Myco-synthesized copper oxide nanoparticles using harnessing metabolites of endophytic fungal strain Aspergillus terreus: an insight into antibacterial, anti-Candida, biocompatibility, anticancer, and antioxidant activities

Biosynthesis of Metal and Metal Oxide Nanoparticles Using Microbial Cultures: Mechanisms, Antimicrobial Activity and Applications to Cultural Heritage

Biogenic Silver and Copper Nanoparticles: Potential Antifungal Agents in Rice and Wheat Crops

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