Eco-friendly and Safe Role of Juniperus procera in Controlling of Fungal Growth and Secondary Metabolites

Abstract

Radial growth Aspergillus flavus and Fusarium oxysporum was determined on medium amended with Juniperus procera methanolic extract. The radial growth of A. flavus and F. oxysporum was significantly reduced at 150 and 200 mg. The precentage of reduction was 16.55%, 48.54% and 48.64%, 59.86% for A. flavus and F. oxysporum, respectively. However, addition of J. procera extract to Carbomar significantly reduced radial growth compared with using Carbomar alone. On the other hand, with addition of the J. procera extract, the productivity percentage of aflatoxins B2, aflatoxins B1, sterigmatocystin, cyclopiazonic acid and fusaric acid was reduced by100, 67.44, 96.28, 60.33 and 8.36%, respectively as a result of applied J. procera extract. Moreover, the extract of J. procera significantly reduced the F. oxysporum colony-forming units (cfu) in the cultivated soil with Raphanus sativus at 5 days. F. oxysporum populations at 100 and 200 mg of J. procera extract were 25.33×103 and 21.33×103 cfu g-1, respectively. However, when added with Carbomar, extract of J. procera strongly reduced F. oxysporum populations (9.33×10 3 cfu g-1). In addition, the J. procera extract reduced the mean disease rating of wilt disease of R. sativus caused by F. oxysporum. Less content of chlorophyll a and b (3.56 and 1.65 mg/g fresh weight, respectively at P<0.01) was detected in infected R. sativus than treated with J. procera extract or Carbomar.

Induction role of chitosan nanoparticles to Anethum graveolens extract against food-borne bacteria, oxidant, and diabetic activities in vitro

“…Our results partially agreed with Ahmed et al (2021) , who recorded the absence of caffeic acid, ellagic acid, kaempferol, and apigenin but chlorogenic acid, catechin, rutin, and quercetin occurred in the extract of A. graveolens . These variances in the flavonoid and phenolic contents may arise from some genetic and ecological differences besides the nutritional and cultivation status of the plants, besides the extraction methods and the used solvents ( Ghany, 2014 ). Most of the detected compounds, either phenolic or flavonoid, documented their validity in the various biological activities as mentioned in the scientific papers.…”

Efficacy of Botanical Fungicides against Curvularia lunata at Molecular Levels

“…C. lunata growth was inhibited by 88.42 and 37.50 % when growth medium was treated with J. procera and A. marina extracts respectively. Recently, Abdelghany [27] reported the antifungal effect of J. procera crude extract against growth of Aspergillus flavus and Fusarium oxysporum. Surprisingly, A. marina extract at low concentrations induced the vegetative growth of C. lunata (Figure 2), but it suppressed the fungal sporogenisis.…”

“…[24]. Juniperus species have been extensively investigated as a source of natural products with potential antibacterial, antifungal and insecticidal activities [25][26][27]. Mariana and Camelia, [28] reported that Juniper oil has inhibition action against Aspergillus niger, Fusarium oxysporum, Monascus purpureus and Penicillium hirsutum.…”

In silico Identification of Ergosterol as a Novel Fungal Metabolite Enhancing RuBisCO Activity in Lycopersicum esculentum

“…Plant activators such as ergosterol [24], methylesterase [25], abscisic acid [26], gibberellic acid, salicylic acid [27] and SO 4 2- [28] and inhibitors such as coronatine [29], aflatoxin [30], aspergillic acid [31], fusaric acid [32] and CA1P [28] were docked. Out of all the activators tested for RuBisCO activity, ergosterol was found to have high binding affinity to RuBisCO LSU and SSU, followed by methylesterase (Table 4).…”