Efficacy of Botanical Fungicides against Curvularia lunata at Molecular Levels

TM, Abdel Ghany; M, Abdel Rahman; E, Shater Moustafa; A, Negm Mohamed

doi:10.4172/2157-7471.1000289

Cited by 11 publications

(9 citation statements)

References 17 publications

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“…Similarly, El-Baz et al (2016) recorded pH 6.5 as the best for silver nanoparticles biosynthesis. Lately, the antimicrobial activity of negatively charged oxygen compounds (pigments) had been reported as the bioactive compounds in the biological control (Abdel Ghany et al 2015). In the present study, the antimicrobial action of AgNPs has been associated with M. purpureus EG crude metabolite compounds chemical properties and their versatility such as deacetylation degree and molecular weight.…”

Section: Antimicrobial Activity Of Agnpsmentioning

confidence: 58%

“…These results disagree with the results by Talapragada et al (2017) that reported that the antimicrobial action of extract containing M. purpureus pigment had shown negative results against E. coli which may be attributed to the type of the media or tested strain. Abdel Ghany et al (2015) reported that culture filtrate of Monascus strain had antibacterial action against Bacillus spp. Other research finds that antimicrobial activity is significantly determined by cell growth conditions and culture medium compositions (Narsing et al 2017).…”

Section: Antimicrobial Activity Of Monascus Pigmentsmentioning

confidence: 99%

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Growth and pigment production of Monascus purpureus EG and its beneficial effects

El‐Fallal

Abo-Zied

El-Zahed

2022

Nova Biotechnol. Chim.

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Pigments play an important role in the pharmaceutical industry as well as in the food industry. Biological synthesis of pigments has attained more revenue for easy extraction, high growth rate and high yield. The production of pigments by Monascus purpureus EG was investigated in several static batch cultures, the most suitable medium for its yellow, orange, and red extracellular pigment production was yeast glucose medium (YG), while malt extract medium (ME) had maximum production for its intracellular pigment. The effect of some physical factors on growth and pigment production was studied. Direct illumination inhibited growth and pigment production. Antimicrobial activities of pigments were observed against selected Gram-negative (G-ve) and Gram-positive (G+ve) bacteria. The antibacterial effects of red pigment on G- and G+ were highly effective compared to yellow and orange pigments. The extracted pigment was used for the reduction of the aqueous silver nitrate into silver nanoparticles (AgNPs). The biosynthesized AgNPs were structurally characterized using UV-VIS Spectra which showed absorption peaks at 437, 453 and 447 nm for pH values 3.5, 6.5 and 9.5, respectively. The optimum pH for the maximum synthesis of nanoparticles was 6.5. It showed no nitrate reductase activity, but the synthesized AgNPs exhibited strong antimicrobial activity against G+ and G- bacteria.

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Section: Antimicrobial Activity Of Agnpsmentioning

confidence: 58%

Section: Antimicrobial Activity Of Monascus Pigmentsmentioning

confidence: 99%

Growth and pigment production of Monascus purpureus EG and its beneficial effects

El‐Fallal

Abo-Zied

El-Zahed

2022

Nova Biotechnol. Chim.

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“…Some bioactive compounds produced by certain plants have fungicidal or fungi toxic activities because of their ability to inhibit the growth of fungi and their spores [5] and also have the ability to block the biosynthetic process of pathogenic metabolite [3]. Citronellal, linalool, and  and  pinenes produced by Cymbopogon inhibited fungal growth effectively [15].…”

Section: Table 3 the Inhibitory Effect Of Botanical Extracts Onmentioning

confidence: 99%

“…However, using synthetic fungicides for a long period created environmental problems and human health [2]. These negative effects can be overcome by using more environmentally friendly pesticides [3], such as plant extracts that contain fungicidal effects [4]. Plant-based fungicides could break down faster than synthetic chemical pesticides, so they do not accumulate for a long time in the environment [5].…”

Section: Introductionmentioning

confidence: 99%

Evaluating the effectiveness of botanical extracts on cotton sclerotial fungi

Yulianti¹,

Hidayah²,

Wijayanti³

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Sclerotium rolfsii, Rhizoctonia solani, and R. bataticola caused damping-off, root rot, and charcoal rot on cotton plant. The fungi produced sclerotia, and hence they are difficult to control. Synthetic fungicides had negative effects on the environment, but fungicidal compounds of botanical extracts were environmentally friendly. This research aimed to test several botanical extracts on the three fungi, i.e., extract from neem seed, the mixture of neem seed with clove oil, and citronella oil with ratio 7:1; 3:1; 1:1. The concentration used for the test were: 500, 1250, 2500, and 3750 ppm. Each of the tested fungi was placed in the middle of PDA medium with the botanical extract. Each treatment was replicated three times. The growth of the tested fungi was measured every two days. Neem extract inhibited the mycelial growth of the three tested fungi 2-40 %. When the neem was mixed with clove oil, its effectiveness escalated to 100 %, especially at ratios 3:1 and 1:1. The mixture of neem extract with citronella oil was less effective than that of neem extract with clove oil. Of the three tested fungi, S. rolfsii was the most vulnerable fungus to the tested botanical extract.

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“…These can be exploited in controlling the growth of fungi consequently inhibiting secondary metabolite production. The effect of plant extracts has been investigated on C. lunata depicting alteration in growth, sporulation, and secondary metabolite pathways ( Ghany TM et al, 2015 ). Many investigations resulted in the screening of a wide variety of plant species/bioactive compounds for their antimicrobial activities and have revealed structurally unique biologically active compounds ( Matasyoh et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Gene expression, molecular docking, and molecular dynamics studies to identify potential antifungal compounds targeting virulence proteins/genes VelB and THR as possible drug targets against Curvularia lunata

Kamboj

Gupta

Kumar

et al. 2022

Front. Mol. Biosci.

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Curvuluria lunata is a melanized fungus pathogenic to both plants and animals including humans, causing from mild, febrile to life-threatening illness if not well treated. In humans, it is an etiological agent of keratomycosis, sinusitis, and onychomycosis in immunocompromised and immunocompetent patients. The development of multiple-drug-resistant strains poses a critical treatment issue as well as public health problem. Natural products are attractive prototypes for drug discovery due to their broad-spectrum efficacy and lower side effects. The present study explores possible targets of natural antifungal compounds (α-pinene, eugenol, berberine, and curcumin) against C. lunata via gene expression analysis, molecular docking interaction, and molecular dynamics (MD) studies. Curcumin, berberine, eugenol, and α-pinene exhibited in vitro antifungal activity at 78 μg/ml, 156 μg/ml, 156 μg/ml, and 1250 μg/ml, respectively. In addition, treatment by these compounds led to the complete inhibition of conidial germination and hindered the adherence when observed on onion epidermis. Several pathogenic factors of fungi are crucial for their survival inside the host including those involved in melanin biosynthesis, hyphal growth, sporulation, and mitogen-activated protein kinase (MAPK) signalling. Relative gene expression of velB, brn1, clm1, and pks18 responsible for conidiation, melanin, and cell wall integrity was down-regulated significantly. Results of molecular docking possessed good binding affinity of compounds and have confirmed their potential targets as THR and VelB proteins. The docked structures, having good binding affinity among all, were further refined, and rescored from their docked poses through 100-ns long MD simulations. The MDS study revealed that curcumin formed a stable and energetically stabilized complex with the target protein. Therefore, the study concludes that the antifungal compounds possess significant efficacy to inhibit C. lunata growth targeting virulence proteins/genes involved in spore formation and melanin biosynthesis.

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Efficacy of Botanical Fungicides against Curvularia lunata at Molecular Levels

Cited by 11 publications

References 17 publications

Growth and pigment production of Monascus purpureus EG and its beneficial effects

Growth and pigment production of Monascus purpureus EG and its beneficial effects

Evaluating the effectiveness of botanical extracts on cotton sclerotial fungi

Gene expression, molecular docking, and molecular dynamics studies to identify potential antifungal compounds targeting virulence proteins/genes VelB and THR as possible drug targets against Curvularia lunata

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