Echinococcus granulosus: The distribution of hydatid fluid antigens in the tissues of the larval stage

La, Yarzábal; Dupas, H; Bout, Daniel; Naquira, F.; Caprón, A

doi:10.1016/0014-4894(77)90068-6

Cited by 16 publications

(13 citation statements)

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“…In previous studies, Ag 5 has been located in the superficial layers of the laminar membrane (Rickard et al 1977) and Ag B has been observed diffusely present throughout the membrane, and in its surface (Yarzabal et al 1977, Rickard et al 1977. A selective permeability of the laminar membrane to Ag B has been argued to explain this different distribution (Yarzabal et al 1977).…”

Section: Discussionmentioning

confidence: 92%

“…Ag 5 was shown to be associated with the parenchymal cells of the protoscoleces, the brood capsule wall and the germinal membrane (Yarzabal et al 1976, Rickard et al 1977, Davies et al 1978, it is also present in the superficial layers of the laminated membrane (Rickard et al 1977). Ag B has been located on a variety of tissues, including the tegument of the protoscoleces (Yarzabal et al 1977, Rickard et al 1977, Davies et al 1978, the brood capsule wall (Rickard et al 1977, Davies ef al. 1978, the germinal membrane (Rickard et al 1977), the parenchyma cells (Rickard et al 1977, Davies et al 1978) and the cyst contents (Yarzabal et al 1977, Davies et al 1978.…”

Section: Introductionmentioning

confidence: 99%

“…Ag B has been located on a variety of tissues, including the tegument of the protoscoleces (Yarzabal et al 1977, Rickard et al 1977, Davies et al 1978, the brood capsule wall (Rickard et al 1977, Davies ef al. 1978, the germinal membrane (Rickard et al 1977), the parenchyma cells (Rickard et al 1977, Davies et al 1978) and the cyst contents (Yarzabal et al 1977, Davies et al 1978.…”

Section: Introductionmentioning

confidence: 99%

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Immunochemical localization of major hydatid fluid antigens in protoscoleces and cysts of Echinococcus granulosus from human origin

Sánchez

March

Mercader

et al. 1991

Parasite Immunology

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Monospecific rabbit antisera obtained through experimental immunization with previously purified proteins were used in the structural localization of two hydatid fluid antigens, antigen 5 and antigen B, in cyst membranes and protoscoleces of E. granulosus from human origin. The antigen-antibody reaction was revealed by an avidin-biotin-peroxidase technique. Antigen 5 was not evident in the laminated membrane of the cyst wall, but it was associated with the germinal membrane of the cyst wall and brood capsules. The parenchyma of invaginated and evaginated protoscoleces was heavily labelled. The tegument, the calcareous corpuscles, the suckers and the hooks did not contain antigen 5. Degenerated protoscoleces were also labelled. Antigen B localization was essentially identical to antigen 5, but degenerated protoscoleces were not recognized by anti-antigen B antiserum. Technical aspects and differences with previously published work are discussed.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immunochemical localization of major hydatid fluid antigens in protoscoleces and cysts of Echinococcus granulosus from human origin

Sánchez

March

Mercader

et al. 1991

Parasite Immunology

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“…At the present time, despite the large number of studies, the parasitic antigens present in HCF that have major immunodiagnostic value in detecting E. granulosus are antigen 5 (Ag5) and antigen B (AgB) [34, 35]. Native Ag5, a 400 kDa thermolabile glycoprotein produces two subunits at 55 and 65 kDa in sodium-dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) under nonreducing conditions and two subunits at 38/39 and 22–24 kDa under reducing conditions [36–38]. The biological role of Ag5 is almost completely unknown, although its elevated concentration in HCF suggests a relevant function in the development of the metacestode.…”

Section: E Granulosus Antigensmentioning

confidence: 99%

Host-Parasite Relationship in Cystic Echinococcosis: An Evolving Story

Siracusano

Delunardo

Teggi

et al. 2012

Clinical and Developmental Immunology

120

101

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The larval stage of Echinococcus granulosus causes cystic echinococcosis, a neglected infectious disease that constitutes a major public health problem in developing countries. Despite being under constant barrage by the immune system, E. granulosus modulates antiparasite immune responses and persists in the human hosts with detectable humoral and cellular responses against the parasite. In vitro and in vivo immunological approaches, together with molecular biology and immunoproteomic technologies, provided us exciting insights into the mechanisms involved in the initiation of E. granulosus infection and the consequent induction and regulation of the immune response. Although the last decade has clarified many aspects of host-parasite relationship in human cystic echinococcosis, establishing the full mechanisms that cause the disease requires more studies. Here, we review some of the recent developments and discuss new avenues in this evolving story of E. granulosus infection in man.

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“…Previous studies focused on livestock such as sheep and cattle. Capron and Yarzabal discovered antigen 5 and antigen B in HCF through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blot analysis 2,3 . Zhu used improved two-dimensional polyacrylamide electrophoresis to identify 111 proteins in the liver HCF of infected sheep 4 .…”

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confidence: 99%

Analysis of the chemical components of hydatid fluid from Echinococcus granulosus

Wang

et al. 2013

Rev. Soc. Bras. Med. Trop.

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Introduction:The aim of this study was to explore the environment of Echinococcus granulosus (E. granulosus) protoscolices and their relationship with their host. Methods: Proteins from the hydatid-cyst fl uid (HCF) from E. granulosus were identifi ed by proteomics. An inductively coupled plasma atomic emission spectrometer (ICP-AES) was used to determine the elements, an automatic biochemical analyzer was used to detect the types and levels of biochemical indices, and an automatic amino acid analyzer was used to detect the types and levels of amino acids in the E. granulosus HCF. Results: I) Approximately 30 protein spots and 21 peptide mass fi ngerprints (PMF) were acquired in the two-dimensional gel electrophoresis (2-DE) pattern of hydatid fl uid; II) We detected 10 chemical elements in the cyst fl uid, including sodium, potassium, calcium, magnesium, copper, and zinc; III) We measured 19 biochemical metabolites in the cyst fl uid, and the amount of most of these metabolites was lower than that in normal human serum; IV) We detected 17 free amino acids and measured some of these, including alanine, glycine, and valine. Conclusions: We identifi ed and measured many chemical components of the cyst fl uid, providing a theoretical basis for developing new drugs to prevent and treat hydatid disease by inhibiting or blocking nutrition, metabolism, and other functions of the pathogen.Keywords ; however, many previously identifi ed components of HCF were not included. HCF proteins are composed of 44% albumin, 39% α-globulin and β-globulin, and 17% γ-globulin 7 . Li determined that liver HCF and lung HCF from sheep and yak contained 17 amino acids, but the total protein level was very low, equivalent to a level of approximately 1-2% in serum 8 . Similarly, the cholesterol level was also found to be low (approximately 12% in serum). Polysaccharides, together with proteins and lipids, were present in sheep liver HCF 9 . Other researchers also detected urea, uric acid, proteins and amino acids, lipids, electrolytes, glucose, glycogen, many trace elements, and proteases, for example, in the HCF 10-13 . In summary, these studies established a baseline assessment of the chemical constituents of HCF.In the past few years, little progress has been made in establishing the chemical constitution of HCF. In this chapter, we report the results of a comprehensive analysis of the environment of the larvae. Because HCF exchanges substances with the host for the survival and reproduction of protoscolexes, an understanding of the larval environment will aid in identifying RESULTS METHODSthe essential components of parasite growth and, potentially, in developing novel methods for preventing E. granulosus infection. Purifi cation of hydatid-cyst fl uidHuman HCF was collected after the surgical removal of fertile cysts from patients with cystic hydatid disease in The General Hospital of Ningxia Medical University. In total, 21 cysts of different sizes were isolated in a germ-free environment, and 55ml of cyst fl uid was aspira...

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Echinococcus granulosus: The distribution of hydatid fluid antigens in the tissues of the larval stage

Cited by 16 publications

References 10 publications

Immunochemical localization of major hydatid fluid antigens in protoscoleces and cysts of Echinococcus granulosus from human origin

Immunochemical localization of major hydatid fluid antigens in protoscoleces and cysts of Echinococcus granulosus from human origin

Host-Parasite Relationship in Cystic Echinococcosis: An Evolving Story

Analysis of the chemical components of hydatid fluid from Echinococcus granulosus

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