Ecdysteroids affectDrosophilaovarian stem cell niche formation and early germline differentiation

Abstract

Ecdysteroids affect Drosophila ovarian stem cell niche formation and early germline differentiationEcdysteroids affect Drosophila ovarian stem cell niche formation and early germline differentiation. The steroid hormone ecdysone regulates germline development and stem cell niche establishment in the Drosophila ovary by modulating TGF-β signalling and cell adhesion.

Novel Tools for Genetic Manipulation of Follicle Stem Cells in the Drosophila Ovary Reveal an Integrin-Dependent Transition from Quiescence to Proliferation

“…However, ptc-lacZ expression also was observed in GFP-labeled cells at the region 2A-2B border and occasionally in cells further to the posterior ( Figure 5J). These results support previous reports that the ptc promoter drives expression in multiple somatic cell populations throughout the germarium (Forbes et al 1996b;Casanueva and Ferguson 2004;Gancz et al 2011;König et al 2011;Sahai-Hernandez and Nystul 2013) and emphasizes the need for caution in using ptc as a marker for distinguishing FSCs from IGS cells and pre-follicle cells.…”

“…ptc-Gal4 and sliGal4 NP1625 promoted UAS-GFP expression in all somatic cells in the anterior half of the germarium, including terminal filament and cap cells, IGS cells, and FSCs ( Figure 3A and Table 1) (Casanueva and Ferguson 2004;Gancz et al 2011;König et al 2011;Sahai-Hernandez and Nystul 2013). In some germaria, the domain of expression extended further to the posterior and included FSC progeny, the early follicle cells (Forbes et al 1996b;Casanueva and Ferguson 2004;Gancz et al 2011;König et al 2011;Sahai-Hernandez and Nystul 2013). Like ptc-Gal4 and sli-Gal4 NP1625 , dally-like (dlp) GMR53H05 -Gal4, dlp GMR61C11 -Gal4, and sgg-Gal4 NP0082 promoted UAS-GFP expression in all anterior somatic cells as well as in FSCs and occasionally in FSC progeny ( Figure 3B).…”

Steroid signaling in mature follicles is important for Drosophila ovulation

“…Our study defined the dynamic expression pattern of Shd and EcR isoforms in late oogenesis and demonstrated the requirement of ecdysteroid signaling in ovulation. Ecdysteroids have also been shown to regulate early germ cell differentiation (35,36), egg chamber progression at stage 8-9 (29)(30)(31), and endocycle-to-gene-amplification switch at stage10B (33). This temporal action of ecdysteroids is reminiscent to their roles in regulating multiple larval and pupal transitions (27,28).…”

“…Ecdysteroids have long been known to play essential roles in developmental transitions, such as larval molting and metamorphosis (27,28). In contrast, the roles of ecdysteroids in adult physiology have just started to emerge (29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39).…”

Drosophila as a model of wound healing and tissue regeneration in vertebrates

“…Different classes of intrinsic factors, translational regulators, chromatin remodeling factors, cell cycle regulators and miRNAs are also required for controlling GSC or SSC self-renewal Hatfield and RuoholaBaker, 2008;Chan and RuoholaBaker, 2010, for review). It has been recently shown that ecdysone functions in GSC niche formation and the control of female GSCs proliferation through interactions with chromatin remodeling factors (Ables and Drummond-Barbosa, 2010;König et al, 2011). These results suggest that steroid hormones and the intrinsic chromatin remodeling machinery could act as potential mechanisms to promote broad transcriptional programs not only involved in ASC self-renewal but also in other processes such as DC or imaginal disc regeneration in Drosophila.…”