Ecdysone receptor expression and activity in adult Drosophila melanogaster

Schwedes, Christoph Cleland; Tulsiani, Siddharth; Carney, Ginger E.

doi:10.1016/j.jinsphys.2011.03.027

Cited by 56 publications

(40 citation statements)

References 52 publications

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“…S2B, S2C). The efficacy of EcR RNAi was confirmed by downregulation of EcR activity reporter EcRE-lacZ (Schwedes et al, 2011) in EcR RNAi -expressing follicle cells (Fig. S2D).…”

Section: Resultsmentioning

confidence: 77%

Regulation of broad by the Notch pathway affects timing of follicle cell development

Jia

Tamori

Pyrowolakis

et al. 2014

Developmental Biology

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During Drosophila oogenesis, activation of Notch signaling in the follicular epithelium (FE) around stage 6 of oogenesis is essential for entry into the endocycle and a series of other changes such as cell differentiation and migration of subsets of the follicle cells. Notch induces the expression of zinc finger protein Hindsight and suppresses homeodomain protein Cut to regulate the mitotic/endocycle (ME) switch. Here we report that broad (br), encoding a small group of zinc-finger transcription factors resulting from alternative splicing, is a transcriptional target of Notch nuclear effector Suppressor of Hairless (Su(H)). The early pattern of Br in the FE, uniformly expressed except in the polar cells, is established by Notch signaling around stage 6, through the binding of Su(H) to the br early enhancer (brE) region. Mutation of the Su(H) binding site leads to a significant reduction of brE reporter expression in follicle cells undergoing the endocycle. Chromatin immunoprecipitation results further confirm Su(H) binding to the br early enhancer. Consistent with its expression in follicle cells during midoogenesis, loss of br function results in a delayed entry into the endocycle. Our findings suggest an important role of br in the timing of follicle cell development, and its transcriptional regulation by the Notch pathway.

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“…S2B, S2C). The efficacy of EcR RNAi was confirmed by downregulation of EcR activity reporter EcRE-lacZ (Schwedes et al, 2011) in EcR RNAi -expressing follicle cells (Fig. S2D).…”

Section: Resultsmentioning

confidence: 77%

Regulation of broad by the Notch pathway affects timing of follicle cell development

Jia

Tamori

Pyrowolakis

et al. 2014

Developmental Biology

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“…Ecdysteroid and DA receptors have been detected in the brain of a few insect species. In D. melanogaster , ecdysteroid nuclear receptors (EcR/USP) are widely expressed in the brain [31]. In the honeybee, they have been detected in the MBs and their expression changed with the foraging behavior [32].…”

Section: Discussionmentioning

confidence: 99%

Involvement of the G-Protein-Coupled Dopamine/Ecdysteroid Receptor DopEcR in the Behavioral Response to Sex Pheromone in an Insect

Abrieux

Debernard²,

Maria³

et al. 2013

PLoS ONE

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Most animals including insects rely on olfaction to find their mating partners. In moths, males are attracted by female-produced sex pheromones inducing stereotyped sexual behavior. The behaviorally relevant olfactory information is processed in the primary olfactory centre, the antennal lobe (AL). Evidence is now accumulating that modulation of sex-linked behavioral output occurs through neuronal plasticity via the action of hormones and/or catecholamines. A G-protein-coupled receptor (GPCR) binding to 20-hydroxyecdysone, the main insect steroid hormone, and dopamine, has been identified in Drosophila (DmDopEcR), and was suggested to modulate neuronal signaling. In the male moth Agrotis ipsilon, the behavioral and central nervous responses to pheromone are age-dependent. To further unveil the mechanisms of this olfactory plasticity, we searched for DopEcR and tested its potential role in the behavioral response to sex pheromone in A. ipsilon males. Our results show that A. ipsilon DopEcR (named AipsDopEcR) is predominantly expressed in the nervous system. The corresponding protein was detected immunohistochemically in the ALs and higher brain centers including the mushroom bodies. Moreover, AipsDopEcR expression increased with age. Using a strategy of RNA interference, we also show that silencing of AipsDopEcR inhibited the behavioral response to sex pheromone in wind tunnel experiments. Altogether our results indicate that this GPCR is involved in the expression of sexual behavior in the male moth, probably by modulating the central nervous processing of sex pheromone through the action of one or both of its ligands.

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“…However, recently alternatively spliced regions in the hinge domain and the ligand-binding domain have also been identified in various crustaceans, including the fiddler crab Uca pugilator (Chung et al 1998a,b, Durica et al 2002, the kuruma prawn Marsupenaeus japonicus (Asazuma et al 2007), the American clawed lobster Homarus americanus (Tarrant et al 2011), the freshwater prawn Macrobrachium nipponense (Shen et al 2013), and the blue crab Callinectes sapidus (Techa & Chung 2013). It has been proposed that different isoforms of EcR had their unique domains, which influence each receptor's ability to activate or repress gene expression, and hence exert different physiological functions (Hopkins et al 2008, Tan & Palli 2008, Schwedes et al 2011.…”

Section: Introductionmentioning

confidence: 99%

Ecdysone receptor in the mud crab Scylla paramamosain: a possible role in promoting ovarian development

Gong¹,

Ye²,

Xie³

et al. 2015

Journal of Endocrinology

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In arthropods, it is known that ecdysteroids regulate molting, limb regeneration, and reproduction through activation of the ecdysone receptor (EcR). However, the ecdysteroid signaling pathway for promotion of ovarian development in crustaceans is still unclear. In this study, three cDNA isoforms of EcR were cloned from the mud crab Scylla paramamosain. qRT-PCR revealed that the SpEcR mRNA was abundant in the eyestalk, ovary and epidermis. During ovarian development, the SpEcR transcripts increased from stage I (undeveloped stage) and reached a peak at stage IV (late vitellogenic stage) before dropping to a lower level at stage V (mature stage). Meanwhile, levels of 20-hydroxyecdysone (20E) in the hemolymph, detected by HPLC-MS, displayed a similar pattern of increase with ovarian development. Results from in situ hybridization indicated that SpEcR mRNA was present in the follicular cells during vitellogenesis. Results from in vivo experiments revealed that 20E at 0.2 mg/g body weight significantly stimulated the expression of SpEcR and vitellogenin (SpVg) in female crabs during the early vitellogenic stage but not during the previtellogenic stage. This was confirmed by results from in vitro experiments which indicated that SpEcR and SpVg expression levels were significantly upregulated in early vitellogenic ovarian explants incubated with 5.0 mM 20E at 3 and 6 h but not in previtellogenic ovarian explants. Finally, results from in vitro gene silencing experiments indicated that the expression of SpEcR and SpVg in the ovary was significantly inhibited by SpEcR dsRNA. All these results together indicated that in S. paramamosain, 20E, and SpEcR, located in the follicular cells, play important roles in the promotion of ovarian development via regulating the expression of SpVg.

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Ecdysone receptor expression and activity in adult Drosophila melanogaster

Cited by 56 publications

References 52 publications

Regulation of broad by the Notch pathway affects timing of follicle cell development

Regulation of broad by the Notch pathway affects timing of follicle cell development

Involvement of the G-Protein-Coupled Dopamine/Ecdysteroid Receptor DopEcR in the Behavioral Response to Sex Pheromone in an Insect

Ecdysone receptor in the mud crab Scylla paramamosain: a possible role in promoting ovarian development

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