Involvement of the G-Protein-Coupled Dopamine/Ecdysteroid Receptor DopEcR in the Behavioral Response to Sex Pheromone in an Insect

Abrieux, Antoine; Debernard, Stéphane; Maria, Annick; Gaertner, Cyril; Anton, Sylvia; Gadenne, Christophe; Duportets, Line

doi:10.1371/journal.pone.0072785

Cited by 33 publications

(43 citation statements)

References 57 publications

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“…Nevertheless, quite a number of studies have successfully performed RNAi in a number of different moth species by using injection (Table 1). Similar to other insect taxa, RNAi based on dsRNA injection has been applied to all life stages in moths: (1) egg stage [15,43]; (2) larval stage [23,24,33]; (3) pupal stage [17,27]; and (4) adult stage [25,31] (Table 1). …”

Section: Methods To Introduce Exogenous Dsrna Into the Mothsmentioning

confidence: 99%

“…Several other genes that relate to reproduction in moths also have been determined by using RNAi, including the DopEcR that relates to sex pheromone response in Agrotis ipsilon [25,99], olfactory receptor in L. dispar [100] and S. litura [34], PBAN [27,36] and PBAN-R [101,102] in many moths that relate to the regulation of sex pheromone biosynthesis, and the FATP and GPAT in B. mori that relate to bombykol biosynthesis [103]. …”

Section: Use Of Rnai For Gene Function Analysis In Mothsmentioning

confidence: 99%

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RNA Interference in Moths: Mechanisms, Applications, and Progress

Wang

Chen

et al. 2016

Genes

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The vast majority of lepidopterans, about 90%, are moths. Some moths, particularly their caterpillars, are major agricultural and forestry pests in many parts of the world. However, some other members of moths, such as the silkworm Bombyx mori, are famous for their economic value. Fire et al. in 1998 initially found that exogenous double-stranded RNA (dsRNA) can silence the homolog endogenous mRNA in organisms, which is called RNA interference (RNAi). Soon after, the RNAi technique proved to be very promising not only in gene function determination but also in pest control. However, later studies demonstrate that performing RNAi in moths is not as straightforward as shown in other insect taxa. Nevertheless, since 2007, especially after 2010, an increasing number of reports have been published that describe successful RNAi experiments in different moth species either on gene function analysis or on pest management exploration. So far, more than 100 peer-reviewed papers have reported successful RNAi experiments in moths, covering 10 families and 25 species. By using classic and novel dsRNA delivery methods, these studies effectively silence the expression of various target genes and determine their function in larval development, reproduction, immunology, resistance against chemicals, and other biological processes. In addition, a number of laboratory and field trials have demonstrated that RNAi is also a potential strategy for moth pest management. In this review, therefore, we summarize and discuss the mechanisms and applications of the RNAi technique in moths by focusing on recent progresses.

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Section: Methods To Introduce Exogenous Dsrna Into the Mothsmentioning

confidence: 99%

Section: Use Of Rnai For Gene Function Analysis In Mothsmentioning

confidence: 99%

RNA Interference in Moths: Mechanisms, Applications, and Progress

Wang

Chen

et al. 2016

Genes

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“…The dsRNA production and injection conditions were established by following the protocol used for the silencing of a Gprotein-coupled dopamine/ecdysteroid receptor (AipsDopEcR) and the ecdysone receptor (AiEcR) in A. ipsilon (Abrieux et al, 2013;Bozzolan et al, 2015). The dsRNAs designed against AiERR (425 bp) and bacterial LacZ (372 bp) were produced with a MEGAscriptV R T7 High Yield Transcription Kit (Ambion) according to the manufacturer's instructions.…”

Section: Rna Interference Experimentsmentioning

confidence: 99%

“…We also examined the effects of different injections (saline, LacZ-dsRNA and AiERR-dsRNA) on the expression level of AipsDopEcR in the brain. This G protein-coupled receptor has been recently shown to be predominantly expressed in the nervous system and especially in AL, and also found to be critical for the performance of the sex pheromone-orientated flight of male A. ipsilon (Abrieux et al, 2013).…”

Section: Rna Interference Experimentsmentioning

confidence: 99%

Evidence for a role of oestrogen receptor‐related receptor in the regulation of male sexual behaviour in the moth Agrotis ipsilon

Bozzolan

Durand

Demondion

et al. 2017

Insect Molecular Biology

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The oestrogen receptor-related receptors (ERRs) are orphan nuclear receptors that were originally identified on the basis of their close homology to the oestrogen receptors. The three mammalian ERR genes participate in the regulation of vital physiological processes including reproduction, development and metabolic homeostasis. Although unique ERRs have been found in insects, data on the function and regulation of these receptors remain sparse. In the present study, a 2095-bp full-length cDNA encoding an ERR, termed AiERR, was isolated from males of the moth Agrotis ipsilon and deposited in the GenBank database under the accession number KT944662. The predicted AiERR protein shared an overall identity of 47-82% with other known insect and mammalian ERR homologues. AiERR exhibited a broad tissue expression pattern with the detection of one transcript of approximately 2 kb in the primary olfactory centres, the antennal lobes (AL). In adult males, the amount of AiERR mRNA in the AL increased concomitantly with age and responses to the female-emitted sex pheromone. Moreover, AiERR knockdown induced an inhibition in the sex pheromone-orientated flight of male. Using A. ipsilon as a model, our study demonstrates that the insect ERR is critical for the performance of male sexual behaviour, probably by acting on central pheromone processing.

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“…Dopamine impacts the peripheral nervous system response to queen mandibular pheromone in worker honeybees, Apis mellifera L., as pheromone response is associated with expression of dopamine receptors in the antennae (Vergoz et al, 2009). A receptor that binds both ecdysteroids and dopamine is expressed in antennal lobes and mushroom bodies of the moth A. ipsilon (Abrieux et al, 2013). Expression of this receptor increases with moth age and sexual maturation, and is essential for central nervous system perception (Abrieux et al, 2014) and behavioural response to sex pheromone (Abrieux et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

The roles of juvenile hormone and biogenic amines on pheromone response plasticity and diapause termination in male Caloptilia fraxinella

Lemmen

Evenden

2016

Entomologia Exp Applicata

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In insects that exhibit a period of delayed reproduction, the timing of mating and reproduction is controlled by environmental conditions that regulate endogenous factors such as hormones and biogenic amines (BAs). Caloptilia fraxinella (Ely) (Lepidoptera: Gracillariidae) undergoes a 9-month reproductive diapause from adult eclosion in the summer until diapause termination the following spring when adults mate. Male response to female sex pheromone is plastic, and is most acute when moths are reproductively active. The aim of this study is to further elucidate the mechanisms involved in the regulation of male response to pheromone in C. fraxinella, and to test whether the application of BAs with and without a juvenile hormone analogue (JHA) to males in different physiological states impacts pheromone responsiveness, as measured by electroantennogram and wind tunnel bioassays. Treatment of male C. fraxinella in reproductive diapause with one application of a JHA induces the highest subsequent pheromone response in the fall, but does not alter pheromone response earlier in reproductive diapause in the summer. The JHAs methoprene and pyriproxyfen similarly enhance pheromone response in the fall. Treatment with methoprene alone or in combination with one of the BAs octopamine, dopamine or serotonin increases male pheromone responsiveness in the fall. The increase in pheromone response can be attributed to methoprene only, as treatment with any of the BAs alone does not enhance male response to pheromone. Biogenic amine treatment lowers male responsiveness to pheromone in some experiments, indicating that there may be a role for BAs in maintaining low pheromone response during reproductive diapause in this species.

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Involvement of the G-Protein-Coupled Dopamine/Ecdysteroid Receptor DopEcR in the Behavioral Response to Sex Pheromone in an Insect

Cited by 33 publications

References 57 publications

RNA Interference in Moths: Mechanisms, Applications, and Progress

RNA Interference in Moths: Mechanisms, Applications, and Progress

Evidence for a role of oestrogen receptor‐related receptor in the regulation of male sexual behaviour in the moth Agrotis ipsilon

The roles of juvenile hormone and biogenic amines on pheromone response plasticity and diapause termination in male Caloptilia fraxinella

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