EcbZIP60, a basic leucine zipper transcription factor from Eleusine coracana L. improves abiotic stress tolerance in tobacco by activating unfolded protein response pathway

Babitha, K. C.; Ramu, S. V.; Nataraja, K.; Sheshshayee, M. S.; Udayakumar, M.

doi:10.1007/s11032-015-0374-6

Cited by 41 publications

(27 citation statements)

References 63 publications

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“…Under optimal conditions, EcbZIP17 transgenic plants showed better growth, including increased plant height, number of leaves, shoot girth, internode distance, and higher pod and seed yield, than WT plants, suggesting the role of EcbZIP17 in vegetative growth and maturation. Previous studies revealed that overexpression of all MTTFs, AtbZIP28 Δ, AtbZIP60 , and AtbZIP17 Δ from Arabidopsis and EcbZIP60 from finger millet, negatively affected plant growth under optimal conditions, but improved tolerance under stress conditions 25 , 26 , 47 . Arabidopsis plants overexpressing ZmbZIP17 showed normal growth under optimal conditions, similar to that of WT plants 52 .…”

Section: Discussionmentioning

confidence: 99%

“…These results showed that EcbZIP17 plays a key role in improving tolerance to multiple abiotic stresses. Previous studies reported that the overexpression of AtbZIP60 and AtbZIP28 in Arabidopsis provided tolerance to salt stress and heat stress, respectively 53 , 54 , whereas the overexpression of EcbZIP60 in tobacco provided tolerance to both salt and drought stress 47 . Many other members of bZIP family TFs also have been reported to provide tolerance to multiple stresses.…”

Section: Discussionmentioning

confidence: 99%

“…Finger millet seedlings at the three-leaf growth stage were subjected to different abiotic stressors, including heat at 42 °C, dehydration by removing seedlings from the medium and keeping them on 3-mm Whatman sheet, sodium chloride (NaCl; 250 mM), mannitol (300 mM), abscisic acid (ABA; 100 µM), hydrogen peroxide (H 2 O 2 ; 25 mM), and DTT (2.5 mM) 47 , 64 , 65 . Seedling samples were collected after 2 h, 4 h, 6 h, 8 h, and 24 h of treatment.…”

Section: Methodsmentioning

confidence: 99%

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The membrane tethered transcription factor EcbZIP17 from finger millet promotes plant growth and enhances tolerance to abiotic stresses

Chopperla

Singh

Raghavendrarao

et al. 2018

Sci Rep

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The occurrence of various stresses, as the outcome of global climate change, results in the yield losses of crop plants. Prospecting of genes in stress tolerant plant species may help to protect and improve their agronomic performance. Finger millet (Eleusine coracana L.) is a valuable source of superior genes and alleles for stress tolerance. In this study, we isolated a novel endoplasmic reticulum (ER) membrane tethered bZIP transcription factor from finger millet, EcbZIP17. Transgenic tobacco plants overexpressing this gene showed better vegetative growth and seed yield compared with wild type (WT) plants under optimal growth conditions and confirmed upregulation of brassinosteroid signalling genes. Under various abiotic stresses, such as 250 mM NaCl, 10% PEG6000, 400 mM mannitol, water withdrawal, and heat stress, the transgenic plants showed higher germination rate, biomass, primary and secondary root formation, and recovery rate, compared with WT plants. The transgenic plants exposed to an ER stress inducer resulted in greater leaf diameter and plant height as well as higher expression of the ER stress-responsive genes BiP, PDIL, and CRT1. Overall, our results indicated that EcbZIP17 improves plant growth at optimal conditions through brassinosteroid signalling and provide tolerance to various environmental stresses via ER signalling pathways.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The membrane tethered transcription factor EcbZIP17 from finger millet promotes plant growth and enhances tolerance to abiotic stresses

Chopperla

Singh

Raghavendrarao

et al. 2018

Sci Rep

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“…In tomato, the negative role of SlbZIP38 in drought and salt stress was linked to higher level of MDA and lower chlorophyll and free proline content in leaves [ 73 ]. In Eleusine coracana , transcripts of EcbZIP60 were highly induced by salt-, drought-, and methyl viologen-induced stress, and promoted abiotic stress tolerance in transgenic tobacco by activating the expression of unfolded protein-responsive pathway genes, including PDIL , CRT1 , and mBiP1 [ 74 ]. Functional analysis of GmbZIP110 from soybean revealed that it can bind to the ACGT motif and modulate the expression of many stress-related genes in transgenic Arabidopsis.…”

Section: Transcription Factors Involved In Abiotic Stress Responsementioning

confidence: 99%

Revisiting the Role of Plant Transcription Factors in the Battle against Abiotic Stress

Khan

Wang

et al. 2018

IJMS

191

118

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Owing to diverse abiotic stresses and global climate deterioration, the agricultural production worldwide is suffering serious losses. Breeding stress-resilient crops with higher quality and yield against multiple environmental stresses via application of transgenic technologies is currently the most promising approach. Deciphering molecular principles and mining stress-associate genes that govern plant responses against abiotic stresses is one of the prerequisites to develop stress-resistant crop varieties. As molecular switches in controlling stress-responsive genes expression, transcription factors (TFs) play crucial roles in regulating various abiotic stress responses. Hence, functional analysis of TFs and their interaction partners during abiotic stresses is crucial to perceive their role in diverse signaling cascades that many researchers have continued to undertake. Here, we review current developments in understanding TFs, with particular emphasis on their functions in orchestrating plant abiotic stress responses. Further, we discuss novel molecular mechanisms of their action under abiotic stress conditions. This will provide valuable information for understanding regulatory mechanisms to engineer stress-tolerant crops.

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“…Technical advances in Setaria can also be useful for other millet species for the purposes of functional complementation of orthologous genes. Two recent studies found a NAC and a bZIP transcription factor from finger millet can enhance abiotic tolerance in rice and tobacco, respectively (Babitha et al, 2015; Rahman et al, 2016). As reverse genetic tools advance in S. viridis , the pace of gene discovery will also accelerate, enabling the identification of candidate genes that can be introduced into other grasses to confer enhanced abiotic stress tolerance.…”

Section: Setaria Viridis As a Model System To Dissect Gene Function Imentioning

confidence: 99%

Setaria viridis as a Model System to Advance Millet Genetics and Genomics

et al. 2016

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Millet is a common name for a group of polyphyletic, small-seeded cereal crops that include pearl, finger and foxtail millet. Millet species are an important source of calories for many societies, often in developing countries. Compared to major cereal crops such as rice and maize, millets are generally better adapted to dry and hot environments. Despite their food security value, the genetic architecture of agronomically important traits in millets, including both morphological traits and climate resilience remains poorly studied. These complex traits have been challenging to dissect in large part because of the lack of sufficient genetic tools and resources. In this article, we review the phylogenetic relationship among various millet species and discuss the value of a genetic model system for millet research. We propose that a broader adoption of green foxtail (Setaria viridis) as a model system for millets could greatly accelerate the pace of gene discovery in the millets, and summarize available and emerging resources in S. viridis and its domesticated relative S. italica. These resources have value in forward genetics, reverse genetics and high throughput phenotyping. We describe methods and strategies to best utilize these resources to facilitate the genetic dissection of complex traits. We envision that coupling cutting-edge technologies and the use of S. viridis for gene discovery will accelerate genetic research in millets in general. This will enable strategies and provide opportunities to increase productivity, especially in the semi-arid tropics of Asia and Africa where millets are staple food crops.

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EcbZIP60, a basic leucine zipper transcription factor from Eleusine coracana L. improves abiotic stress tolerance in tobacco by activating unfolded protein response pathway

Cited by 41 publications

References 63 publications

The membrane tethered transcription factor EcbZIP17 from finger millet promotes plant growth and enhances tolerance to abiotic stresses

The membrane tethered transcription factor EcbZIP17 from finger millet promotes plant growth and enhances tolerance to abiotic stresses

Revisiting the Role of Plant Transcription Factors in the Battle against Abiotic Stress

Setaria viridis as a Model System to Advance Millet Genetics and Genomics

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