EBV mononucleosis in children in modern conditions

Hakizimana, Jean-Claude; Timchenko, V. N.; Shakmaeva, Maria A.; Kaplina, Tatyana A.; Subbotina, M. D.; Bannova, S. L.; Федорова, А. В.; Sukhovetskaya, Vera F.; Павлова, Е. Б.; Павлова, Н. В.

doi:10.22627/2072-8107-2020-19-2-23-28

Cited by 9 publications

(5 citation statements)

References 4 publications

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“…У детей, поступивших в стационар позднее, предварительный диагноз на догоспитальном и госпитальном этапах поставлен значительно точнее. Более поздние сроки госпитализации от начала заболевания, несмотря на среднетяжелое течение инфекции, были характерны для детей 7-17 лет, что ранее также отмечалось в работах других отечественных авторов [10,26]. Поздняя госпитализация детей может иметь неблагоприятные последствия.…”

Section: Discussionunclassified

“…Хотя максимальные показатели заболеваемости регистрируют в раннем детском и дошкольном возрасте, некоторые исследователи в последние годы отмечают эпидемиологическое неблагополучие в старших возрастных группах 7-17 лет [8]. К тому же среди них с большей частотой диагностируют тяжелые формы ИМ [9,10].…”

Section: Introductionunclassified

“…В отношении лабораторных критериев ИМ следует констатировать, что диагностическая значимость тестирования атипичных мононуклеаров в периферической крови или гетерофильных антител существенно варьирует [12]. Гематологические и биохимические показатели крови являются неспецифическими для диагностики ИМ, но они используются при оценке степени тяжести заболевания и его осложнений [9,10].…”

Section: Introductionunclassified

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Diagnostic value of Epstein – Barr virus DNA quantification in blood leukocytes in children with infectious mononucleosis

Попкова

Филатова

Soboleva

et al. 2022

jour

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Aim. To determine the threshold value of the Epstein-Barr virus (EBV) viral load (VL) in blood leukocytes to improve the laboratory diagnostics of infectious mononucleosis in children.Materials and methods. EBV DNA quantification in blood leukocytes in children aged 1-17 years (n=163) was determined by real-time polymerase chain reaction. VL were compared in groups of EBV mononucleosis (n=67), non-EBV mononucleosis (n=25) and healthy donors (n=25). Threshold was determined based on VL data from children with active and latent EBV infection. The R program and the RStudio environment were used for satistic analysis.Results. EBV DNA is found in blood leukocytes in infectious mononucleosis not associated with EBV and in healthy virus carriers, however VL in these groups is significantly lower than in patients with EBV mononucleosis (p<0.001). The threshold value was determined – 41 copies/105 cells (or 1.6 lg of EBV DNA/105 cells), which was characterized by acceptable values of specificity and sensitivity (0.90 and 0.85, respectively) of laboratory diagnostics. High EBV VL (equal to or above the set threshold) is associated with an 8.5-fold increased risk of detecting active EBV infection compared to children who have a low VL (below a set threshold) (RR 8.5; 95% CI: 3.7–19.7, p<0.001).Conclusion. In general, the results obtained create prerequisites for more intensive implementation of quantitative studies of EBV DNA in blood leukocytes, both in the context of improving the early diagnosis of infectious mononucleosis and its etiological interpretation, and in terms of detailing the features of the course of EBV infection.

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Section: Discussionunclassified

Section: Introductionunclassified

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Diagnostic value of Epstein – Barr virus DNA quantification in blood leukocytes in children with infectious mononucleosis

Попкова

Филатова

Soboleva

et al. 2022

jour

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“…Для подтверждения диагноза используют методы специфической лабораторной диагностики: определение серологических маркеров (антитела класса IgM и G) методом иммуноферментного анализа, выявление ДНК возбудителя молекулярно-генетическим исследованием (полимеразная цепная реакция -ПЦР) [5,8,9,15,18,20].…”

Section: оригинальные статьиunclassified

Acute EBV mononucleosis in children of various ages hospitalized in the infectious hospital of the St. Petersburg State Pediatric Medical University in 2017–2020

Fedorova,

Sukhovetskaya,

Timchenko

et al. 2023

Pediatr (SPb)

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BACKGROUND: The problem of acute EBV-mononucleosis is one of the most urgent in pediatrics. In the structure of the incidence of infectious mononucleosis, the proportion of the child population is 70–80%, children 4–8 years old are more often ill. Maternal antibodies protect an infant only in the first months of life, primary infection may occur at 3 months of age, and a manifest form of acute EBV-mononucleosis may develop at 6 months of age. AIM: The aim of the study was to analyze peculiarities of acute EBV-mononucleosis in children of different ages hospitalized in the infectious diseases hospital of the Saint Petersburg State Pediatric Medical University. MATERIALS AND METHODS: The article presents data on 376 children aged 1 to 18 years with a diagnosis of Acute EBV mononucleosis, hospitalized in the period 2017–2020. In the infectious diseases department No. 1 of Saint Petersburg State Medical University. Etiological interpretation was carried out using ELISA (determination of IgM to VCA in the blood) and detection of EBV DNA in the blood by PCR. RESULTS: In 89.6% of cases, the disease was of moderate severity. Acute onset of the disease was noted in 69.7% of cases, more often in children from 1 to 10 years old. Intoxication syndrome was observed with the same frequency in all age groups. Febrile fever was observed in 96.0% of patients. The syndrome of acute tonsillitis, lymphadenitis was detected in all patients. The phenomena of adenoiditis and hepatomegaly were more pronounced in younger children. Splenomegaly was recorded in 74.5% of cases, more often than hepatomegaly. Cytolysis syndrome was observed in every second child. In older age groups, there was a higher level of hyperenzymemia and a pronounced mononuclear reaction in the hemogram. CONCLUSION: All the analyzed patients had a typical clinical picture of acute EBV mononucleosis with a characteristic syndrome complex. In most cases, the disease was of moderate severity, severe severity was recorded in 8.2% — mainly in older children. Involvement of the liver and spleen in the pathological process simultaneously took place in every second patient. In older age groups, there was a higher level of hyperfermentemia and a pronounced mononuclear reaction in the hemogram.

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“…HPV is a relatively small virus (55 nm in diameter) that possesses a non-enveloped circular double-stranded genomic DNA of approximately 8 kb in size [14][15][16]. Another oncovirus, EBV, which is an oncogenic herpesvirus, is associated with a spectrum of diseases, ranging from infectious mononucleosis to various neoplastic conditions [17][18][19]. These neoplastic diseases include lymphoproliferative disorders in immunocompromised individuals, Burkitt's lymphoma, Hodgkin's lymphoma, and nasopharyngeal carcinoma [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Structure-Based Discovery of Potential HPV E6 and EBNA1 Inhibitors: Implications for Cervical Cancer Treatment

Broni,

Ashley,

Velazquez

et al. 2024

Computation

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Cervical cancer is the fourth most diagnosed cancer and the fourth leading cause of cancer death in women globally. Its onset and progression have been attributed to high-risk human papillomavirus (HPV) types, especially 16 and 18, while the Epstein–Barr virus (EBV) is believed to also significantly contribute to cervical cancer growth. The E6 protein associated with high-risk HPV strains, such as HPV16 and HPV18, is known for its role in promoting cervical cancer and other anogenital cancers. E6 proteins contribute to the malignant transformation of infected cells by targeting and degrading tumor suppressor proteins, especially p53. On the other hand, EBV nuclear antigen 1 (EBNA1) plays a crucial role in the maintenance and replication of the EBV genome in infected cells. EBNA1 is believed to increase HPV E6 and E7 levels, as well as c-MYC, and BIRC5 cellular genes in the HeLa cell line, implying that HPV/EBV co-infection accelerates cervical cancer onset and growth. Thus, the E6 and EBNA1 antigens of HPV and EBV, respectively, are attractive targets for cervical cancer immunotherapy. This study, therefore, virtually screened for potential drug candidates with good binding affinity to all three oncoviral proteins, HPV16 E6, HPV18 E6, and EBNA1. The compounds were further subjected to ADMET profiling, biological activity predictions, molecular dynamics (MD) simulations, and molecular mechanics Poisson–Boltzmann surface area (MM/PBSA) calculations. A total of six compounds comprising ZINC000013380012, ZINC000070454124, ZINC000014588133, ZINC000085568136, ZINC000095909247, and ZINC000085597263 demonstrated very strong affinity (≤−60 kJ/mol) to the three oncoviral proteins (EBNA1, HPV16 E6, and HPV18 E6) after being subjected to docking, MD, and MM/PBSA. These compounds demonstrated relatively stronger binding than the controls used, inhibitors of EBNA1 (VK-1727) and HPV E6 (baicalein and gossypetin). Biological activity predictions also corroborated their antineoplastic, p53-enhancing, Pin1 inhibitory, and JAK2 inhibitory activities. Further experimental testing is required to validate the ability of the shortlisted compounds to silence the insidious effects of HPV E6 and EBNA1 proteins in cervical cancers.

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EBV mononucleosis in children in modern conditions

Cited by 9 publications

References 4 publications

Diagnostic value of Epstein – Barr virus DNA quantification in blood leukocytes in children with infectious mononucleosis

Diagnostic value of Epstein – Barr virus DNA quantification in blood leukocytes in children with infectious mononucleosis

Acute EBV mononucleosis in children of various ages hospitalized in the infectious hospital of the St. Petersburg State Pediatric Medical University in 2017–2020

Structure-Based Discovery of Potential HPV E6 and EBNA1 Inhibitors: Implications for Cervical Cancer Treatment

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