Early transcriptomic response of mouse adrenal gland and Y-1 cells to dexamethasone

Abstract

Glucocorticoids have short- and long-term effects on adrenal gland function and development. RNA sequencing (RNA-seq) was performed to identify early transcriptomic responses to the synthetic glucocorticoid, dexamethasone (Dex), in vitro and in vivo. In total, 1,711 genes were differentially expressed in the adrenal glands of the 1-h Dex-treated mice. Among them, only 113 were also considered differentially expressed genes (DEGs) in murine adrenocortical Y-1 cells treated with Dex for 1 h. Gene ontology analysis showed that the upregulated DEGs in the adrenal gland of the 1-h Dex-treated mice were highly associated with the development of neuronal cells, suggesting the adrenal medulla had a rapid response to Dex. Interestingly, only 4.3% of Dex-responsive genes in the Y-1 cell line under Dex treatment for 1 h were also differentially expressed under Dex treatment for 24 h. The heatmaps revealed that most early-responsive DEGs in Y-1 cells during 1 h of treatment exhibited a transient response. The expression of these genes under treatment for 24 h returned to basal levels similar to that during control treatment. In summary, this research compared the rapid transcriptomic effects of Dex stimulation in vivo and in vitro. Notably, adrenocortical Y-1 cells had a transient early response to Dex treatment. Furthermore, the DEGs had a minimal overlap in the 1-h Dex-treated group in vivo and in vitro.

Is membrane androgen and estrogen receptor signaling imperative in the governing function of the adrenal cortex in the Eurasian beaver (Castor fiber L.)?

“…There are still not many studies available. The research are related mainly to the adrenals of mice and rats, including in vitro systems where the mostly adrenal development and stress responses were analyzed (Abou Nader et al, 2022;Aşır et al, 2022;Bornstein et al, 2004;Friedrich et al, 2021;Zheng et al, 2022). In wild animals, adrenal gland physiology was studied scarcely by us and others (Barszcz et al, 2016;Ekele et al, 2014;Kotula-Balak et al, 2023;Olukole et al, 2016;Pawlicki et al, 2023;Sabbi et al, 2020).…”