Early transcriptome profile of goat peripheral blood mononuclear cells (PBMCs) infected with peste des petits ruminant's vaccine virus (Sungri/96) revealed induction of antiviral response in an interferon independent manner

Manjunath, Siddappa; Saxena, Shikha; Mishra, Bina; Santra, Lakshman; Sahu, Anamika; Wani, Sajad A.; Tiwari, Ashok Kumar; Mishra, Bishnu Prasad; Singh, Raj Kumar; Janga, Sarath Chandra; Kumar, Girish

doi:10.1016/j.rvsc.2019.03.014

Cited by 10 publications

(11 citation statements)

References 52 publications

(43 reference statements)

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“…With respect to the upregulated genes expressed in a common way between strains, most of them appear to be IFN antiviral response genes that are induced at a similar level between infections. Previous studies have also shown that these interferon-stimulated genes (ISGs) are upregulated in the early stages of PPRV infection ( 25 , 26 ). In addition, these ISGs help to strengthen host defence and reduce PPRV replication in goats and cattle ( 48 ).…”

Section: Discussionmentioning

confidence: 99%

“…Immune cells (lymphocytes, monocytes and dendritic cells) are responsible for transport of the virus to the tonsillar tissue and lymph nodes draining the initial site of infection where extensive viral replication occurs before propagation to epithelial and lymphoid tissues of other organs ( 23 ). In-vitro studies using peripheral blood mononuclear cells (PBMCs) have shed some light on the immune response to PPRV infections ( 24 – 26 ). In PBMCs infected with PPRV apoptotic indices were found including peripheral condensation of chromatin, nucleus and cell fragmentation leading to the formation of apoptotic bodies ( 27 ).…”

Section: Introductionmentioning

confidence: 99%

“…Transcriptome analysis of PBMCs infected by the PPRV vaccine strain Sungri/96 showed the dysregulation of both immune regulatory pathways and the expression of genes encoding the transcription factors that govern the response to viral infection ( 24 ). Innate immune molecules and antiviral genes seem to play an important role at the earliest state in infection of PBMCs, suggesting a link between innate and adaptive immune response to PPRV infection ( 25 , 26 ).…”

Section: Introductionmentioning

confidence: 99%

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Identification of Differential Responses of Goat PBMCs to PPRV Virulence Using a Multi-Omics Approach

et al. 2021

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Peste des petits ruminants (PPR) is an acute transboundary infectious viral disease of small ruminants, mainly sheep and goats. Host susceptibility varies considerably depending on the PPR virus (PPRV) strain, the host species and breed. The effect of strains with different levels of virulence on the modulation of the immune system has not been thoroughly compared in an experimental setting so far. In this study, we used a multi-omics approach to investigate the host cellular factors involved in different infection phenotypes. Peripheral blood mononuclear cells (PBMCs) from Saanen goats were activated with a T-cell mitogen and infected with PPRV strains of different virulence: Morocco 2008 (high virulence), Ivory Coast 1989 (low virulence) and Nigeria 75/1 (live attenuated vaccine strain). Our results showed that the highly virulent strain replicated better than the other two in PBMCs and rapidly induced cell death and a stronger inhibition of lymphocyte proliferation. However, all the strains affected lymphocyte proliferation and induced upregulation of key antiviral genes and proteins, meaning a classical antiviral response is orchestrated regardless of the virulence of the PPRV strain. On the other hand, the highly virulent strain induced stronger inflammatory responses and activated more genes related to lymphocyte migration and recruitment, and inflammatory processes. Both transcriptomic and proteomic approaches were successful in detecting viral and antiviral effectors under all conditions. The present work identified key immunological factors related to PPRV virulence in vitro.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of Differential Responses of Goat PBMCs to PPRV Virulence Using a Multi-Omics Approach

et al. 2021

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“…However, in recent years, there has been growing evidence that IFITM3 is closely related to the development and prognosis of many tumors (28,30). For example, Li et al Studies show that expression is significantly up-regulated in colon cancer and has an important relationship with its development and metastasis (27).…”

Section: Discussionmentioning

confidence: 99%

“…The interferon-inducible transmembrane protein 3 (IFITM3, also known as 18u) gene (together with IFITM1 and IFITM2) belongs to the IFITM gene family that is clustered on chromosome 11 and is also considered to be an antiviral gene (25,26). In recent years, an increasing number of studies have found that IFITM3 expression is associated with the prognosis of a variety of tumors, such as gastrointestinal tumors and glioma, and is involved in cell migration, invasion, Proliferation, apoptosis and tumorigenesis (27)(28)(29)(30). there are significant research value.…”

Section: Introductionmentioning

confidence: 99%

TUG1 as ceRNA combined with miR-29a to promotes the expression of IFITM3 in hepatocellular carcinoma

Liu

Feng

Liao

et al. 2020

Preprint

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Background: Numerous studies have shown that TUG1 has an important relationship with tumorigenesis. TUG1 is highly expressed in most tumors and can promote tumor development. However, the role of TUG1 in hepatocellular carcinoma (HCC) remains to be studied. miR-29a plays a tumor suppressor role in a variety of tumors, and there is a relationship between TUG1 and miR-29a, but the specific relationship and mechanism of action are still unclear. miR-29a can inhibit the expression of IFITM3. However, the regulatory relationship between these three components requires elucidation. This study aimed to investigate the regulatory relationship between TUG1, miR-29a, and IFITM3 in human hepatocarcinogenesis.Methods: The expression levels of TUG1 and miR-29a in tumor tissues and adjacent non-tumor tissues of 41 HCC patients were detected by real-time quantitative polymerase chain reaction. The migration and invasion of liver cancer cells were studied by a wound healing assay and the Transwell method. The apoptosis rate of hepatocarcinoma cells was detected by flow cytometry, and the proliferation rate of hepatoma cells was detected by the EdU method. Immunofluorescence was selected to detect the expression of TUG1 and IFITM3 in HCC-LM3 and HL-7702. The relationship between TUG1 and miR-29a was detected using a double luciferase report and FISH. Tumors were established in vivo by subcutaneous injection of hepatocellular carcinoma cells into nude mice and injection of these cells into the tail vein. Western blotting was used to quantify the biomarkers. Results: TUG1 expression increased significantly in both tumor tissues and HCC cells. The expression of miR-29a in liver cancer tissues was also significantly lower than that in normal human liver tissues. The expression of TUG1 in HCC tissue samples was negatively correlated with that of miR-29a. Moreover, the expression of TUG1 was positively correlated with the expression of IFITM3. TUG1 can regulate the migration, invasion, apoptosis, and proliferation of HCC lines in vitro and regulate the development of tumors in vivo. Knocking down TUG1 will increase miR-29a expression, and thus, weaken the invasion, migration, and proliferation of HCC cells and enhance their apoptosis. miR-29a can affect the occurrence and progression of liver cancer through IFITM3. It was found that TUG1 regulates IFITM3 in HCC cells via miR-29a, and its expression affects cell invasion, migration, proliferation, and apoptosis.Conclusion: As a CeRNA, TUG1 competitively binds mir-29a to regulate IFITM3 and promote the development of liver cancer. Downregulation of TUG1 can significantly inhibit the migration, invasion, and proliferation of liver cancer cells, and TUG1 is expected to serve as a key gene to improve the prognosis of patients.

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Applications of Omics Technologies in Livestock Production, Improvement and Sustainability

Maru,

Kumar

2024

Sustainable Agriculture Reviews

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Early transcriptome profile of goat peripheral blood mononuclear cells (PBMCs) infected with peste des petits ruminant's vaccine virus (Sungri/96) revealed induction of antiviral response in an interferon independent manner

Abstract: Early transcriptome profile of goat peripheral blood mononuclear cells (PBMCs) infected with peste des petits ruminant's vaccine virus (Sungri/96) revealed induction of antiviral response in an interferon independent manner. Research in Veterinary Science, 124, 166-177.

Cited by 10 publications

References 52 publications

Identification of Differential Responses of Goat PBMCs to PPRV Virulence Using a Multi-Omics Approach

Identification of Differential Responses of Goat PBMCs to PPRV Virulence Using a Multi-Omics Approach

TUG1 as ceRNA combined with miR-29a to promotes the expression of IFITM3 in hepatocellular carcinoma

Applications of Omics Technologies in Livestock Production, Improvement and Sustainability

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