Early stage mineralization in tissue engineering mapped by high resolution X-ray microdiffraction

Campi, Gaetano; Ricci, Antonella; Guagliardi, Antonietta; Giannini, Cinzia; Lagomarsino, S.; Cancedda, Ranieri; Mastrogiacomo, M.; Cedola, Alessia

doi:10.1016/j.actbio.2012.05.034

Cited by 34 publications

(28 citation statements)

References 27 publications

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“…The evaluation of bone ingrowth into various scaffolds has been demonstrated. [16][17][18] In one study, the mineralization stages at different distances from the scaffold interface were monitored using X-ray and provided insight into the complicated mineral deposition steps at the organic-mineral interface. 17 Also, micro-CT has been employed to evaluate the optimal culture conditions for bone mineralization of various scaffolds in vitro.…”

Section: Figmentioning

confidence: 99%

Imaging Strategies for Tissue Engineering Applications

Nam

Ricles

Suggs

et al. 2015

Tissue Engineering Part B: Reviews

116

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Section: Figmentioning

confidence: 99%

Imaging Strategies for Tissue Engineering Applications

Nam

Ricles

Suggs

et al. 2015

Tissue Engineering Part B: Reviews

116

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“…As known from the literature, the building of hydroxyapatite Ca 10 (PO 4 ) 6 (OH) 2 proceeds via several stages with various calcium phosphate phases as precursors [48]. Amorphous calcium phosphate as well as octa calcium phosphate are discussed as potential biologically relevant precursors of hydroxyapatite in bone [48].…”

mentioning

confidence: 99%

“…Amorphous calcium phosphate as well as octa calcium phosphate are discussed as potential biologically relevant precursors of hydroxyapatite in bone [48]. The different calcium phosphate phases are characterized by their chemical formula and their Ca/P ratio [49].…”

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confidence: 99%

Applicability of ToF-SIMS for monitoring compositional changes in bone in a long-term animal model

Henß

Rohnke

Khassawna

et al. 2013

J. R. Soc. Interface.

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Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a wellestablished technique in material sciences but has not yet been widely explored for implementation in life sciences. Here, we demonstrate the applicability and advantages of ToF-SIMS analysis for the study of minerals and biomolecules in osseous tissue. The locally resolved analysis of fragment ions deriving from the sample surface enables imaging and differentiation of bone tissue and facilitates histology on non-stained cross sections. In a rat model, bilateral ovariectomy combined with either a multi-deficiency diet or steroid treatment was carried out to create osteoporotic conditions. We focused our study on the Ca content of the mineralized tissue and monitored its decline. Calcium mass images of cross sections show the progressive degenerative changes in the bone. We observed a decreased Ca concentration in the edge region of the trabeculae and a decline in the Ca/P ratio. Additionally, we focused on the non-mineralized matrix and identified fragment ions that are characteristic for the collagen matrix. We observed trabeculae with wide ranges of nonmineralized collagen for the diet group owing to an impaired mineralization process. Here, the advantage of coeval monitoring of collagen and minerals indicated an osteomalacic model rather than an osteoporotic one.

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“…Furthermore, this system could be particularly important as a scaffold for the growth of controlled networks of biological molecules [36][37][38].…”

Section: Resultsmentioning

confidence: 99%

Controlling the Cassie-to-Wenzel Transition: an Easy Route towards the Realization of Tridimensional Arrays of Biological Objects

et al. 2014

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Abstract:In this paper we provide evidence that the Cassie-to-Wenzel transition, despite its detrimental effects on the wetting properties of superhydrophobic surfaces, can be exploited as an effective micro-fabrication strategy to obtain highly ordered arrays of biological objects. To this purpose we fabricated a patterned surface wetted in the Cassie state, where we deposited a droplet containing genomic DNA. We observed that, when the droplet wets the surface in the Cassie state, an array of DNA filaments pinned on the top edges between pillars is formed. Conversely, when the Cassie-to-Wenzel transition occurs, DNA can be pinned at different height between pillars. These results open the way to the realization of tridimensional arrays of biological objects.

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Early stage mineralization in tissue engineering mapped by high resolution X-ray microdiffraction

Cited by 34 publications

References 27 publications

Imaging Strategies for Tissue Engineering Applications

Imaging Strategies for Tissue Engineering Applications

Applicability of ToF-SIMS for monitoring compositional changes in bone in a long-term animal model

Controlling the Cassie-to-Wenzel Transition: an Easy Route towards the Realization of Tridimensional Arrays of Biological Objects

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