Early signaling events in the heat stress response of Pyropia haitanensis revealed by phosphoproteomic and lipidomic analyses

Wang, Wenlei; Zheng, Hongyan; Wen, Jian Kang; Xu, Kai; Xu, Yan; Ji, Dehua; Chen, Changsheng; Xie, Chaotian

doi:10.1016/j.algal.2022.102837

Cited by 4 publications

(2 citation statements)

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“…Kong et al (2021) revealed that PyMAPK2 expression in P. yezoensis is rapidly induced after exposure to various abiotic stresses [ 43 ]. Our previous study also found that eight P. haitanensis MAPKs are phosphorylated in response to heat shock stress [ 44 ]. In the present study, MAPK cascade-related genes, 2 of 2 MKK3 genes, 11 of 11 MPK8 genes, and 1 of 1 MPK4 gene, had upregulated expression levels in both P. haitanensis strains under LI conditions ( Figure 8 ), suggesting that MAPK cascades are rapidly activated in P. haitanensis blades in response to LI stress.…”

Section: Discussionmentioning

confidence: 96%

Investigating the Mechanisms Underlying the Low Irradiance-Tolerance of the Economically Important Seaweed Species Pyropia haitanensis

Zhang

Bao

et al. 2023

Life

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Pyropia haitanensis, one of the most economically and ecologically important seaweed species, is often exposed to persistent or transient low irradiance (LI), resulting in limited yield and quality. However, the mechanisms mediating P. haitanensis responses to LI are largely unknown. In this study, LI-tolerant (LIT) and LI-sensitive (LIS) P. haitanensis strains were compared regarding their physiological and transcriptomic changes induced by 1 and 4 days of LI (5 μmol photons/m2·s). The results indicated that the inhibition of photomorphogenesis and decreases in photosynthesis and photosynthetic carbon fixation as the duration of LI increased are the key reasons for retarded blade growth under LI conditions. A potential self-amplifying loop involving calcium signaling, phosphatidylinositol signaling, reactive oxygen species signaling, and MAPK signaling may be triggered in blades in response to LI stress. These signaling pathways might activate various downstream responses, including improving light energy use, maintaining cell membrane stability, mitigating oxidative damage, to resist LI stress. Additionally, the LIT strain maintained transcriptional homeostasis better than the LIS strain under LI stress. Specifically, photosynthesis and energy production were relatively stable in the LIT strain, which may help to explain why the LIT strain was more tolerant to LI stress than the LIS strain. The findings of this study provide the basis for future investigations on the precise mechanisms underlying the LI stress tolerance of P. haitanensis.

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Section: Discussionmentioning

confidence: 96%

Investigating the Mechanisms Underlying the Low Irradiance-Tolerance of the Economically Important Seaweed Species Pyropia haitanensis

Zhang

Bao

et al. 2023

Life

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“…Many studies have been devoted to the molecular mechanism behind the stress tolerance of this economic seaweed. Under external stress, a potential self-amplifying signaling loop involving Ca 2+ , phosphatidylinositol, and reactive oxygen species (ROS) is generated in this alga ( Zheng et al, 2019 ; Suda and Mikami, 2020 ; Wang et al, 2020 ; Guan et al, 2022 ), and the rapid, unique Ca 2+ signal is then decoded by calmodulin (CaM) to activate various CaM-dependent kinases ( Brawley et al, 2017 ; Wang et al, 2022 ). Potential downstream pathways, such as the antioxidant system, are then activated to maintain redox homeostasis ( Wang et al, 2018 ; Wang et al, 2019a ; Wang et al, 2020a ; Wang et al, 2020b ), while heat shock proteins (HSPs), the ubiquitin–proteasome system, and protein processing systems are induced to maintain intracellular protein synthesis, folding, and degradation homeostasis ( Wang et al, 2019b ; Wen et al, 2020 ; Niu et al, 2022 ; Zheng et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

PhbZIP2 regulates photosynthesis-related genes in an intertidal macroalgae, Pyropia haitanensis, under stress

Zhang,

Zeng,

Xie

et al. 2024

Front. Mol. Biosci.

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Intertidal macroalgae are important research subjects in stress biology. Basic region-leucine zipper transcription factors (bZIPs) play an important regulatory role in the expression of target genes under abiotic stress. We herein identified a bZIP2 gene PhbZIP2 to regulate abiotic stress tolerance in Pyropia haitanensis, a representative intertidal macroalgal species. Cloning and sequencing of the cDNA characterized a BRLZ structure and an α coiled-coil structure between amino acids and Expression of PhbZIP2 was detected to upregulate under both high temperature and salt stresses. A DAP-seq analysis revealed the PhbZIP2-binding motifs of (T/C)TCCA(C/G) and A (A/G)AAA (G/A), which differed from the conserved motifs in plants. Overexpression of PhbZIP2 was indicative of a high temperature and salt stress tolerances in transgenic Chlamydomonas reinhardtii. It was suggested that PhbZIP2 was probably involved in regulating expression of the photosynthetic-related genes and the response to the abiotic stresses in P. haitanensis, which provide new insights for elucidating efficient adaptation strategies of intertidal macroalgae.

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Light intensity influences the glycerolipid remodeling of Gracilariopsis lemaneiformis in response to short-term high temperature stress

Zhang,

Li,

Cui

et al. 2024

Aquaculture

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Early signaling events in the heat stress response of Pyropia haitanensis revealed by phosphoproteomic and lipidomic analyses

Cited by 4 publications

References 84 publications

Investigating the Mechanisms Underlying the Low Irradiance-Tolerance of the Economically Important Seaweed Species Pyropia haitanensis

Investigating the Mechanisms Underlying the Low Irradiance-Tolerance of the Economically Important Seaweed Species Pyropia haitanensis

PhbZIP2 regulates photosynthesis-related genes in an intertidal macroalgae, Pyropia haitanensis, under stress

Light intensity influences the glycerolipid remodeling of Gracilariopsis lemaneiformis in response to short-term high temperature stress

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