Early screening of dentin caries using the methods of Micro-Raman and laser-induced fluorescence spectroscopy

Development of a new approach to diagnosis of the early fluorosis forms by means of FTIR and Raman microspectroscopy

“…The analysis of active vibrations in Raman spectra was performed basing on several sources where hard dental tissue in normal and pathology was investigated by Raman scattering 14 , 15 , 17 , 20 , 27 , 30 , 35 , 42 – 44 . According to the literature data, the spectra include the most intense vibrations associated with ν 1 and ν 3 PO 4 3− CHAP localised at 962.6 cm −1 and 1006.2, 1030, 1047.4 cm −1 respectively 14 , 20 , 34 , 35 , 45 – 48 .…”

“…Taking into account the requirements of microspectroscopic examination methods for sample geometry, plane-parallel segments of teeth were prepared as reported previously 20 , 27 . A special unit with a diamond disc and water cooling was used to separate the prepared teeth into segments (Fig.…”

“…Since the condition of human teeth is determined by changes occurring in the phase composition of the tissue at the micro and nano level, the use of spectroscopic methods of molecular identification is the most promising and sensitive tool to precisely assess such changes in the dental enamel [24][25][26] . Raman spectroscopy is most often used for diagnostics of dental diseases as a non-destructive method of analysis, which allows to obtain direct data regarding the local atomic structure, chemical and molecular composition of biological objects 18,20,24,[27][28][29] . Also, the hard tissue of a human tooth can be investigated by infrared spectroscopy methods [30][31][32][33][34] , which due to minimal external influences, has not been modified by the method 35 .…”

Engineering of a Biomimetic Interface between a Native Dental Tissue and Restorative Composite and Its Study Using Synchrotron FTIR Microscopic Mapping

“…Flat-parallel segments of the restored tooth samples were prepared for FTIR micro-mapping as previously described [ 42 , 43 ]. Tooth samples were separated using a low-speed, water-cooled diamond saw, and the resulting hard tissue layers were gently sanded and polished using diamond abrasive.…”

Spectroscopic signature of the pathological processes of carious dentine based on FTIR investigations of the oral biological fluids

“…The natural reaction of dentine to carious attack, especially at the early stages of pathology development, is the focus of some of the most state-of-the-art investigations [3,5,6]. Currently, these changes can be controlled mainly by a set of rapid analysis techniques based on saliva analysis [7][8][9] and gingival crevicular fluid [10,11], or inflammatory factors according to serum analysis [12][13][14].…”