Spectroscopic signature of the pathological processes of carious dentine based on FTIR investigations of the oral biological fluids

Seredin, P. V.; Goloshchapov, D. L.; Ippolitov, Yuri; Vongsvivut, Jitraporn

doi:10.1364/boe.10.004050

Cited by 10 publications

(11 citation statements)

References 42 publications

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“…Figure 1 A shows the mean spectra of human saliva for samples processed by different forms after collection by method A. Table 2 shows the respective band assignments 8,20,21 . The group C has higher intensity at bands 1033, 1076 (nuclear material), 1240, 1405, and 1551 cm -1 .…”

Section: Resultsmentioning

confidence: 99%

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Saliva Preparation Method Exploration for ATR-FTIR Spectroscopy: Towards Bio-fluid Based Disease Diagnosis

et al. 2020

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Saliva has garnered a lot of interest as a non-invasive, easy to collect, and biochemical rich sample for Attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) based disease diagnosis. Although large number of studies have explored its potential, the preparation methods used differ greatly. For large scale clinical studies to aid translation into clinics, collection/processing methodology needs to be standardized. Therefore, in this study, we have explored different saliva collection (spitting-method A/cotton soaking-method B) and processing protocols (unprepared-TS, supernatant from centrifugation-CS, and drying-C) to find which gives the best ATR-FTIR signals. Analysis showed highest proteins, carbohydrates, amino acids, and nucleic acid + proteins/lipids in BTS, BCS, ACS, and BC, respectively. Notably, only BC shows 1377 cm-1 nucleic acid band is also uniquely identified in multivariate analysis. We conclude that collection-processing protocol should be based on biochemical that best gives a differential diagnosis.

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Section: Resultsmentioning

confidence: 99%

“…Amide II (CN stretching, NH bending) band components of proteins Seredin et al 21 Júnior et al 3 Badea et al 8…”

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confidence: 99%

Saliva Preparation Method Exploration for ATR-FTIR Spectroscopy: Towards Bio-fluid Based Disease Diagnosis

et al. 2020

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“…Raman spectroscopy is most often used for diagnostics of dental diseases as a non-destructive method of analysis, which allows to obtain direct data regarding the local atomic structure, chemical and molecular composition of biological objects 18,20,24,[27][28][29] . Also, the hard tissue of a human tooth can be investigated by infrared spectroscopy methods [30][31][32][33][34] , which due to minimal external influences, has not been modified by the method 35 .…”

Section: This Study Is Aimed At Investigating the Features Of Mineralmentioning

confidence: 99%

“…The use of infrared microspectroscopy with synchrotron radiation makes it possible to clarify the molecular composition and study local changes in the mineral-organic matrix of dental enamel from the areas less than 10 µm 2 39 . Moreover, in any case the applicability of synchrotron IR-microspectroscopy to the dental tissue analysis is more convenient than Raman microspectroscopy, even though the Raman microscopy is also an excellent approach to vibrational spectroscopy, with an equivalent, or often a better, spatial resolution than synchrotron infrared microscopy 31 , 33 , 37 , 40 .…”

Section: Introductionmentioning

confidence: 99%

Development of a new approach to diagnosis of the early fluorosis forms by means of FTIR and Raman microspectroscopy

Seredin

Goloshchapov

Ippolitov

et al. 2020

Sci Rep

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This study is aimed at investigating the features of mineralization of the enamel apatite at initial stages of fluorosis development. Samples of teeth with intact and fluorotic enamel in an early stage of the disease development (Thylstrup–Fejerskov Index = 1–3) were studied by Raman scattering and FTIR using Infrared Microspectroscopy beamline at Australian Synchrotron equipment. Based on the data obtained by optical microspectroscopy and calculation of the coefficient R [A-type/B-type], which represents the ratio of carbonation fraction of CO32−, replacing phosphate or hydroxyl radicals in the enamel apatite lattice, the features of mineralization of enamel apatite in the initial stages of development of the pathology caused by an increased content of fluorine in the oral cavity were established. Statistical analysis of the data showed significant differences in the mean values of R [A-type/B-type] ratio between the control and experimental groups for surface layers (p < 0.01). The data obtained are potentially significant as benchmarks in the development of a new approach to preventive diagnostics of the development of initial and clinically unregistered stages of human teeth fluorosis, as well as personalized control of the use of fluoride-containing caries-preventive agents.

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“…As a consequence, many works have proposed the FTIR technique as a complementary spectroscopic method to support diagnostic investigation of cytological samples [1][2][3][4][5]. In addition, FTIR has also been considered in clinical practice for biological tissues [6][7][8] and biofluids [9][10][11] analysis, as well as to monitor the response of cells and tissues to radiotherapy [12] and chemotherapy [13] treatments.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of Different Breast Cell Lines on Glass Substrate by Means of Fourier Transform Infrared Spectroscopy

Lasalvia

Capozzi

Perna

2021

Sensors

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Fourier transform infrared (FTIR) micro-spectroscopy has been attracting the interest of many cytologists and histopathologists for several years. This is related to the possibility of FTIR translation in the clinical diagnostic field. In fact, FTIR spectra are able to detect changes in biochemical cellular components occurring when the cells pass to a pathological state. Recently, this interest has increased because it has been shown that FTIR spectra carried out just in the high wavenumber spectral range (2500–4000 cm−1), where information mainly relating to lipids and proteins can be obtained, are able to discriminate cell lines related to different tissues. This possibility allows to perform IR absorption measurements of cellular samples deposited onto microscopy glass slides (widely used in the medical environment) which are transparent to IR radiation only for wavenumber values larger than 2000 cm−1. For these reasons, we show that FTIR spectra in the 2800–3000 cm−1 spectral range can discriminate three different cell lines from breast tissue: a non-malignant cell line (MCF10A), a non-metastatic adenocarcinoma cell line (MCF7) and a metastatic adenocarcinoma cell line (MDA). All the cells were grown onto glass slides. The spectra were discriminated by means of a principal component analysis, according to the PC1 component, whose values have the opposite sign in the pairwise score plots. This result supports the wide studies that are being carried out to promote the translation of the FTIR technique in medical practice, as a complementary diagnostic tool.

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Spectroscopic signature of the pathological processes of carious dentine based on FTIR investigations of the oral biological fluids

Cited by 10 publications

References 42 publications

Saliva Preparation Method Exploration for ATR-FTIR Spectroscopy: Towards Bio-fluid Based Disease Diagnosis

Saliva Preparation Method Exploration for ATR-FTIR Spectroscopy: Towards Bio-fluid Based Disease Diagnosis

Development of a new approach to diagnosis of the early fluorosis forms by means of FTIR and Raman microspectroscopy

Discrimination of Different Breast Cell Lines on Glass Substrate by Means of Fourier Transform Infrared Spectroscopy

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