Early risk prognosis of free-flap transplant failure by quantitation of the macrophage colony-stimulating factor in patient plasma using 2-dimensional liquid-chromatography multiple reaction monitoring-mass spectrometry

Yang, Jing‐Zhi; Finke, Juliane C.; Yang, Juncong; Percy, Andrew J.; Fritschen, Uwe von; Borchers, Christoph H.; Glocker, Michael O.

doi:10.1097/md.0000000000004808

Cited by 3 publications

(4 citation statements)

References 56 publications

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“…Despite differing from rather routinely used two value regimes (below-above cut-offs) for separation of samples/individuals, separation of FGR from CTRL/SGA was successfully employed. Examples of other clinical studies in which it was found that a three value regime was suitable for fulfilling the task to separate two conditions are "early risk prognosis of free-flap transplant failure", "MGMT promoter methylation for selecting glioblastoma patients into trials omitting Temozolomide", and "diagnostic criteria for high-dimensional metabolic data in newborn screening for medium-chain acyl-CoA dehydrogenase deficiency" [22,32,33], but also with sFlt-1/PlGF ratio (<38 and >85) [34,35] and PlGF measurements alone to predict still-birth FGR (<12 and >100) [36]. In agreement with the obtained PCA results, good separation was achieved with the cumulative score discriminator of 1.0 as well.…”

Section: Discussionmentioning

confidence: 99%

“…To determine "best cut-off" values for quotients A, B, and C (see Raw Data Processing) that can be used to classify an individual spectrum (patient) as either belonging to the FGR I or the CTRL I group, a Youden index analysis was performed [17,22,23]. The first independent measurement series MS1 from both the per protocol set (PPS) for CTRL I (n = 14) and FGR I (n = 14) were chosen as training set "O" (Scheme 1 and Supplemental Scheme S1).…”

Section: Youden Index Analyses For Determining Cut-off Valuesmentioning

confidence: 99%

“…The sensitivity and specificity [25] were calculated for each "test cut-off" point. In addition, at each "test cut-off" value, the Youden index (J = sensitivity + specificity − 1) was determined [17,22,23]. The highest J value (Jmax) in the list of samples determined the best discrimination threshold for the quotient A values, i.e., the "best cut-off" value within the samples within this data set.…”

Section: Youden Index Analyses For Determining Cut-off Valuesmentioning

confidence: 99%

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Precision Diagnostics by Affinity-Mass Spectrometry: A Novel Approach for Fetal Growth Restriction Screening during Pregnancy

Okai

Russ

Wölter

et al. 2020

JCM

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Fetal growth restriction (FGR) affects about 3% to 8% of pregnancies, leading to higher perinatal mortality and morbidity. Current strategies for detecting fetal growth impairment are based on ultrasound inspections. However, antenatal detection rates are insufficient and critical in countries with substandard care. To overcome difficulties with detection and to better discriminate between high risk FGR and low risk small for gestational age (SGA) fetuses, we investigated the suitability of risk assessment based on the analysis of a recently developed proteome profile derived from maternal serum in different study groups. Maternal serum, collected at around 31 weeks of gestation, was analyzed in 30 FGR, 15 SGA, and 30 control (CTRL) pregnant women who delivered between 31 and 40 weeks of gestation. From the 75 pregnant women of this study, 2 were excluded because of deficient raw data and 2 patients could not be grouped due to indeterminate results. Consistency between proteome profile and sonography results was obtained for 59 patients (26 true positive and 33 true negative). Of the proteome profiling 12 contrarious grouped individuals, 3 were false negative and 9 were false positive cases with respect to ultrasound data. Both true positive and false positive grouping transfer the respective patients to closer surveillance and thorough pregnancy management. Accuracy of the test is considered high with an area-under-curve value of 0.88 in receiver-operator-characteristics analysis. Proteome profiling by affinity-mass spectrometry during pregnancy provides a reliable method for risk assessment of impaired development in fetuses and consumes just minute volumes of maternal peripheral blood. In addition to clinical testing proteome profiling by affinity-mass spectrometry may improve risk assessment, referring pregnant women to specialists early, thereby improving perinatal outcomes.

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Section: Discussionmentioning

confidence: 99%

Section: Youden Index Analyses For Determining Cut-off Valuesmentioning

confidence: 99%

Section: Youden Index Analyses For Determining Cut-off Valuesmentioning

confidence: 99%

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Precision Diagnostics by Affinity-Mass Spectrometry: A Novel Approach for Fetal Growth Restriction Screening during Pregnancy

Okai

Russ

Wölter

et al. 2020

JCM

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“…These results indicate the sequential function of endogenous MCSF [51], IL8, and IL6 in wound healing which in case of overexpression may be clinically managed by administering appropriate medication, i.e., management of an individuals' systemic inflammatory status to reduce or even prevent conspicuities and complications during perioperative and postoperative periods. Concomitant medication may become an important if not indispensable part of state-of-the-art flap surgery in the future.…”

Section: Issues In Flap Surgerymentioning

confidence: 99%

Plasma Cytokine and Growth Factor Profiling during Free Flap Transplantation

Finke¹,

Yang²,

Bredell³

et al. 2018

Issues in Flap Surgery

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Ischemia and reperfusion (I/R) is an unavoidable condition during free flap transplantation. Restoration of blood flow is usually associated with a profound inflammatory response. Cytokines and growth factors are the functional proteins which exert their specific influence on injury or repair during the healing period. Plasma concentrations of 18 cytokines and growth factor proteins (IL6, IL8, IP10, TNFα, MCP1, Fractalkine, GRO, bFGF, GMCSF, IFNg, MIP1a, VEGF, sCD40L, IL10, TGFα, IL1β, IL12P40, and TNFβ) have been analyzed with respect to I/R status during microsurgery tissue transplantation in both, artery and vein, from patients by multiplexed immunoassay. Both technical feasibility and biostatistics data analysis approaches were thoroughly assessed. It has been found that, from all investigated proteins, the venous plasma levels of IL6 significantly increased during the ischemia period and mostly sustained their high levels during reperfusion, while venous plasma levels of IL8 showed in general a significant increase in the ischemia period followed by a rapid decrease in the reperfusion period. In conclusion, these findings direct toward an active involvement of tissue-resting leukocytes which may become therapeutic targets for concomitant medication in flap surgery to improve wound healing.

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Proteome analysis reveals that de novo regenerated mucosa over fibula flap-reconstructed mandibles resembles mature keratinized oral mucosa

et al. 2018

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Early risk prognosis of free-flap transplant failure by quantitation of the macrophage colony-stimulating factor in patient plasma using 2-dimensional liquid-chromatography multiple reaction monitoring-mass spectrometry

Abstract: Supplemental Digital Content is available in the text

Cited by 3 publications

References 56 publications

Precision Diagnostics by Affinity-Mass Spectrometry: A Novel Approach for Fetal Growth Restriction Screening during Pregnancy

Precision Diagnostics by Affinity-Mass Spectrometry: A Novel Approach for Fetal Growth Restriction Screening during Pregnancy

Plasma Cytokine and Growth Factor Profiling during Free Flap Transplantation

Proteome analysis reveals that de novo regenerated mucosa over fibula flap-reconstructed mandibles resembles mature keratinized oral mucosa

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