Proteome analysis reveals that de novo regenerated mucosa over fibula flap-reconstructed mandibles resembles mature keratinized oral mucosa

Kumar, Vijaya; James, Bonney Lee; Russ, Manuela; Mikkat, Stefan; Suresh, Amritha; Kämmerer, Peer W.; Glocker, Michael O.

doi:10.1016/j.oraloncology.2018.01.017

Cited by 9 publications

(7 citation statements)

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“…Mass spectrometric analysis of peptide mixtures was performed in duplicate on a Synapt G2S mass spectrometer (Waters, Manchester, UK) using Masslynx version 4.1, coupled to a nanoAcquity UPLC system (Waters, Manchester, UK) via a NanoLockSpray ion source using a PicoTip Emitter (New Objective, Woburn, MA, USA) as described [ 41 , 46 ]. Mobile phase A contained 0.1% formic acid in water, and mobile phase B contained 0.1% formic acid in acetonitrile.…”

Section: Methodsmentioning

confidence: 99%

“…For the database search, a database containing 29,944 protein sequences of the Rattus norvegicus proteome ( ; UniProt release 2020_06) appended with the sequences of ClpB_ECOLI (P63284) and porcine trypsin was used. Mass spectrometric raw data file processing, protein identification, and label-free quantification of identified proteins were performed using the Progenesis QI for Proteomics software package, version 4.1 (Nonlinear Dynamics, Newcastle upon Tyne, UK) as previously described [ 46 ]. The following search parameters were set: trypsin as digestion reagent, two missing cleavage sites allowed, carbamidomethylation of cysteines as a fixed modification, and oxidation of methionine residues as variable modification.…”

Section: Methodsmentioning

confidence: 99%

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Memory-Enhancing Effects of Origanum majorana Essential Oil in an Alzheimer’s Amyloid beta1-42 Rat Model: A Molecular and Behavioral Study

et al. 2020

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Origanum L. (Lamiaceae) is an important genus of medicinal and aromatic plants used in traditional medicine since ancient times as culinary herbs and remedies. The aim of the present study was to evaluate the chemical composition, as well as the biochemical and cellular activities of freshly prepared Origanum majorana L. essential oil (OmEO) in an Alzheimer’s disease (AD) amyloid beta1-42 (Aβ1-42) rat model. OmEO (1% and 3%) was inhaled for 21 consecutive days, while Aβ1-42 was administered intracerebroventricularly to induce AD-like symptoms. Our data demonstrate that OmEO increased antioxidant activity and enhanced brain-derived neurotrophic factor (BDNF) expression, which in concert contributed to the improvement of cognitive function of animals. Moreover, OmEO presented beneficial effects on memory performance in Y-maze and radial arm-maze tests in the Aβ1-42 rat AD model.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Memory-Enhancing Effects of Origanum majorana Essential Oil in an Alzheimer’s Amyloid beta1-42 Rat Model: A Molecular and Behavioral Study

et al. 2020

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“…Peri‐implant soft tissue management was carried out at the time of interim prosthesis loading using the technique of sub‐periosteal dissection with denture guided epithelial regeneration. The above‐mentioned procedure is described in detail elsewhere (Kumar, et al, ; Kumar, Jacob, & Kuriakose, ).…”

Section: Methodsmentioning

confidence: 99%

Masticatory efficiency of implant‐supported removable partial dental prostheses in patients with free fibula flap reconstructed mandibles: A split‐mouth, observational study

Kumar

Srinivasan

2018

Clinical Oral Implants Res

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In patients with surgically reconstructed mandibles, a normal masticatory function can be successfully achieved with a rehabilitation of the reconstructed side with implant-supported removable partial dental prostheses. Implant-supported prostheses should be actively advocated in patients with reconstructive surgeries to restore their masticatory function, extend their food choices, and improve their overall oral health-related quality of life.

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“…The Coomassie-stained 1D-SDS gel band with GST-cCPB2 was worked up to generate tryptic peptides according to published protocols (Sinz et al 2002 ; Konus et al 2013 ; Röwer et al 2018 ). Mass spectrometric analysis of peptide mixtures was performed in duplicate on a Synapt G2S mass spectrometer (Waters, Manchester, UK) using MassLynx version 4.1, coupled to a nanoACQUITY UPLC system (Waters MS-Technologies, Manchester, UK) via a NanoLockSpray ion source using a PicoTip Emitter (New Objective, Woburn, MA, USA) as described elsewhere (Sinz et al 2002 ; Kumar et al 2018 ). MS E data were processed using ProteinLynx Global SERVER version 2.3 (Waters MS-Technologies).…”

Section: Methodsmentioning

confidence: 99%

Clostridium perfringens Beta2 toxin forms highly cation-selective channels in lipid bilayers

et al. 2021

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Clostridium perfringens is a potent producer of a variety of toxins. Well studied from these are five toxins (alpha, Beta (CPB), epsilon, iota and CPE) that are produced by seven toxinotype strains (A–G) of C. perfringens. Besides these toxins, C. perfringens produces also another toxin that causes necrotizing enterocolitis in piglets. This toxin termed consensus Beta2 toxin (cCPB2) has a molecular mass of 27,620 Da and shows only little homology to CPB and no one to the other toxins of C. perfringens. Its primary action on cells remained unknown to date. cCPB2 was heterogeneously expressed as fusion protein with GST in Escherichia coli and purified to homogeneity. Although cCPB2 does not exhibit the typical structure of beta-stranded pore-forming proteins and contains no indication for the presence of amphipathic alpha-helices we could demonstrate that cCPB2 is a pore-forming component with an extremely high activity in lipid bilayers. The channels have a single-channel conductance of about 700 pS in 1 M KCl and are highly cation-selective as judged from selectivity measurements in the presence of salt gradients. The high cation selectivity is caused by the presence of net negative charges in or near the channel that allowed an estimate of the channel size being about 1.4 nm wide. Our measurements suggest that the primary effect of cCPB2 is the formation of cation-selective channels followed by necrotic enteritis in humans and animals. We searched in databases for homologs of cCPB2 and constructed a cladogram representing the phylogenetic relationship to the next relatives of cCPB2.

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Proteome analysis reveals that de novo regenerated mucosa over fibula flap-reconstructed mandibles resembles mature keratinized oral mucosa

Cited by 9 publications

References 50 publications

Memory-Enhancing Effects of Origanum majorana Essential Oil in an Alzheimer’s Amyloid beta1-42 Rat Model: A Molecular and Behavioral Study

Memory-Enhancing Effects of Origanum majorana Essential Oil in an Alzheimer’s Amyloid beta1-42 Rat Model: A Molecular and Behavioral Study

Masticatory efficiency of implant‐supported removable partial dental prostheses in patients with free fibula flap reconstructed mandibles: A split‐mouth, observational study

Clostridium perfringens Beta2 toxin forms highly cation-selective channels in lipid bilayers

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