Early pregnancy diagnosis using a commercial ELISA test based on pregnancy-associated glycoproteins in Holstein-Friesian heifers and lactating cows

Kaya, Muhsin; Köse, Mehmet; Bozkaya, Faruk; Mutlu, Hasan; Uçar, Eyyüp Hakan; Atlı, Mehmet Osman

doi:10.3906/vet-1602-41

Cited by 12 publications

(13 citation statements)

References 24 publications

(31 reference statements)

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“…Between Days 26 and 58 after AI, the sensitivity, specificity, positive predictive value, and negative predictive value of the inhouse PAGs ELISA tests varied between 94% and 100%, 77% and 94.2%, 90.7% and 97.8%, and 91.2% and 97.1%, respectively (Table 4). Mercadante et al [81] found higher PAGs concentrations (in-house ELISA) in primiparous cows compared with multiparous pregnant cows (in agreement with Ricci et al [36], while Kaya et al [82] found similar differences between heifers and lactating cows), during the second and later breeding compared with the first breeding postpartum, in cows experiencing clinical metritis, metabolic problems and left displaced abomasum after calving compared with cows not experiencing those clinical diseases and in cases of greater milk yield, while the body condition score did not influence it. According to Dufour et al [38], the accuracy of the PAG test (commercial milk ELISA) was not influenced by parity, the number of days, since the last breeding, and the level of milk production, while Ricci et al [37] found a negative correlation between plasma and milk PAG concentration (commercial ELISA) and milk production.…”

Section: In-house Pags Elisa Testssupporting

confidence: 69%

Recent Possibilities for the Diagnosis of Early Pregnancy and Embryonic Mortality in Dairy Cows

Szenci

2021

Animals

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One of the most recent techniques for the on-farm diagnosis of early pregnancy (EP) in cattle is B-mode ultrasonography. Under field conditions, acceptable results may be achieved with ultrasonography from Days 25 to 30 post-AI. The reliability of the test greatly depends on the frequency of the transducer used, the skill of the examiner, the criterion used for a positive pregnancy diagnosis (PD), and the position of the uterus in the pelvic inlet. Non-pregnant animals can be selected accurately by evaluating blood flow in the corpus luteum around Day 20 after AI, meaning we can substantially improve the reproductive efficiency of our herd. Pregnancy protein assays (PSPB, PAG-1, and PSP60 RIA, commercial ELISA or rapid visual ELISA tests) may provide an alternative method to ultrasonography for determining early pregnancy or late embryonic/early fetal mortality (LEM/EFM) in dairy cows. Although the early pregnancy factor is the earliest specific indicator of fertilization, at present, its detection is entirely dependent on the use of the rosette inhibition test; therefore, its use in the field needs further developments. Recently found biomarkers like interferon-tau stimulated genes or microRNAs may help us diagnose early pregnancy in dairy cows; however, these tests need further developments before their general use in the farms becomes possible.

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Section: In-house Pags Elisa Testssupporting

confidence: 69%

Recent Possibilities for the Diagnosis of Early Pregnancy and Embryonic Mortality in Dairy Cows

Szenci

2021

Animals

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“…P regnancy maintenance is a clear economic factor in dairy animals since most of the pregnancy losses occur during the early stages of pregnancy (Garcıa-Ispierto et al, 2013;Commun et al, 2016). Therefore, timely pregnancy diagnosis is very important for sound reproductive management in herd health (Green et al, 2005;Kaya et al, 2016). Several techniques are being used to diagnose pregnancy in dairy herd such as ultrasound, palpation per rectum, estrone sulfate, milk progesterone assay, and blood tests.…”

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confidence: 99%

Early Pregnancy Diagnosis using Pregnancy-Associated Glycoproteins in the Serum of Pregnant Ruminants

Khan¹,

Khan²,

Ahmad³

et al. 2019

PJZ

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“…Other studies have also found similar PAGs concentrations in dairy heifers during the 4th week of gestation using the same commercial PAG ELISA test kit. 34 Serum progesterone concentrations of the pregnant heifers in the Ovsynch Group were lower (4.70 ± 0.17 ng/mL) than those found in the control (5.37 ± 0.08 ng/mL), or the PG (5.34 ± 0.13 ng/mL) groups (p < 0.001; Figure 1). There are reports that suggest that GnRH-based protocols are associated with low blood progesterone concentrations.…”

Section: Serum Pags and Progesterone Concentrationsmentioning

confidence: 83%

“…16 Particularly, for pregnancy diagnosis through the PAGs assay, when the S-N value was ≥ 0.300 the samples were deemed as positive (pregnant), and when the S-N value was below 0.300 the samples were considered negative (nonpregnant). 34 Serum progesterone concentrations were determined by a commercial bovine progesterone ELISA test kit (Wuhan Fine Biological Technology Co., Ltd., China), in accordance with the manufacturer's instructions.…”

Section: Measurement Of Serum Pags and Progesterone Concentrationsmentioning

confidence: 99%

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Pregnancy-associated glycoproteins (PAGs) and progesterone concentrations in Holstein heifers following two methods of estrus syncronization

et al. 2019

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Pregnancy-associated glycoproteins (PAGs) and progesterone concentrations were assessed in Holstein heifers 28 days post-insemination, following estrus synchronization with either a double prostaglandin (PG) injection or the ovsynch protocol. Animals between 15 and 20 months of age, with a 3-3.5 BCS, were randomly assigned to three groups: Heifers in the control group (n = 70) were monitored for estrus behaviour for 21 days and inseminated when detected in standing heat; Estrus of heifers in the Ovsynch group (n = 70)) was synchronized following the Ovsynch protocol, and timed artificial insemination was performed 16 h after the completion of treatment; Animals in the PG group (n = 70) received two prostaglandin-F2α injections 11 days apart, were monitored for estrus behaviour for 2-5 days following the last injection, and inseminated when in heat. Serum PAGs and progesterone concentrations were measured in pregnant heifers (control group n = 25; ovsynch group n = 22; PG group n = 21) on day 28 after artificial insemination (AI). Mean serum PAGs concentrations (OD: optical density) were similar between groups (control = 3.37 ± 0.148, Ovsynch = 3.21 ± 0.136, and PG = 3.34 ± 0.143; p > 0.05). However, serum progesterone concentrations were found to be lower in the ovsynch group (4.70 ± 0.17 ng/mL) when compared to the PG (5.34 ± 0.13 ng/mL) or the control (5.37 ± 0.08 ng/mL) groups (p < 0.001). There was no correlation between serum PAGs and progesterone levels at day 28 post-insemination, or between early pregnancy PAGs concentrations and fetal death rates. Further research, including multiple sampling time points throughout gestation, after estrus synchronization protocols is warranted, to determine if there are associated temporal changes in PAGs and progesterone profiles that could impact reproductive parameters in dairy heifers.

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Early pregnancy diagnosis using a commercial ELISA test based on pregnancy-associated glycoproteins in Holstein-Friesian heifers and lactating cows

Cited by 12 publications

References 24 publications

Recent Possibilities for the Diagnosis of Early Pregnancy and Embryonic Mortality in Dairy Cows

Recent Possibilities for the Diagnosis of Early Pregnancy and Embryonic Mortality in Dairy Cows

Early Pregnancy Diagnosis using Pregnancy-Associated Glycoproteins in the Serum of Pregnant Ruminants

Pregnancy-associated glycoproteins (PAGs) and progesterone concentrations in Holstein heifers following two methods of estrus syncronization

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