Early postfertilization ovule culture in Alstroemeria L. and barriers to interspecific hybridization

Jeu, M.J. de; Jacobsen, E.

doi:10.1007/bf00035934

Cited by 35 publications

(9 citation statements)

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“…The hybrids and A. inodora were diploid (2n = 2x = 16). Two days after pollination, embryo rescue was applied as described by De Jeu & Jacobsen (1995). The ovules were dissected 2-3 days after pollination, placed on MS90 medium (Murashige & Skoog, 1962) and transferred 6 weeks later to MS30 (Murashige & Skoog, 1962) to induce germination of the embryos.…”

Section: Plant Materials and Backcrossing Of The Interspecific Hybridmentioning

confidence: 99%

“…The genomes of the species are highly differentiated, especially between Chilean and Brazilian species. Such differentiation is evident at three levels: (i) sexual hybridization between species is generally not possible without the help of embryo rescue (Buitendijk et al, 1995;De Jeu & Jacobsen, 1995); (ii) compared with homologous chromosome pairing in the parental species, homoeologous chromosome pairing in the interspecific hybrids is greatly reduced (Ramanna 1991(Ramanna , 1992Lu & Bridgen, 1997); (iii) the genomes of individual species clearly differ with respect to the molecular organization of repetitive DNA sequences among the Chilean species as well as between the Chilean and Brazilian species .…”

Section: Introductionmentioning

confidence: 99%

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Homoeologous chromosome pairing in the distant hybrid Alstroemeria aurea × A. inodora and the genome composition of its backcross derivatives determined by fluorescence in situ hybridization with species-specific probes

et al. 1999

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A distant hybrid between two diploid species (2n = 2x = 16), Alstroemeria aurea and A. inodora, was investigated for homoeologous chromosome pairing, crossability with A. inodora and chromosome transmission to its BC 1 offspring. Fluorescence in situ hybridization (FISH) with two species-specific probes, A001-I (A. aurea specific) and D32-13 (A. inodora specific), was used to analyse chromosome pairing in the hybrid and the genome constitution of its BC 1 progeny plants. High frequencies of associated chromosomes were observed in both genotypes of the F 1 hybrid, A1P2-2 and A1P4. In the former, both univalents and bivalents were found at metaphase I, whereas the latter plant also showed tri-and quadrivalents. Based on the hybridization sites of DNA probes on the chromosomes of both parental species, it was established that hybrid A1P4 contains a reciprocal translocation between the short arm of chromosome 1 and the long arm of chromosome 8 of A. inodora. Despite regular homoeologous chromosome pairing in 30% of the pollen mother cells, both hybrids were highly sterile. They were backcrossed reciprocally with one of the parental species, A. inodora. Two days after pollination, embryo rescue was applied and, eventually, six BC 1 progeny plants were obtained. Among these, two were aneuploids (2n = 2xǹ1 = 17) and four were triploids (2n = 3x = 24). The aneuploid plants had originated when the interspecific hybrid was used as a female parent, indicating that n eggs were functional in the hybrid. In addition, 2n gametes were also functional in the hybrid, resulting in the four triploid BC 1 plants. Of these four plants, three had received 2n pollen grains from the hybrid and one a 2n egg. Using FISH, homoeologous crossing over between the chromosomes of the two parental species in the hybrid was clearly detected in all BC 1 plants. The relevance of these results for the process of introgression and the origin of n and 2n gametes are discussed.

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Section: Plant Materials and Backcrossing Of The Interspecific Hybridmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Homoeologous chromosome pairing in the distant hybrid Alstroemeria aurea × A. inodora and the genome composition of its backcross derivatives determined by fluorescence in situ hybridization with species-specific probes

et al. 1999

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“…Traditional targets of Alstroemeria breeding have primarily focused on the improvement of important horticultural traits, such as flower color and disease tolerance. The mutation breeding techniques successfully developed for Alstroemeria in the 1970s and 1980s (Broerjes and van Harten 1988) have been a tremendous contribution, as have the embryo rescue techniques developed in recent years, which have overcome the interspecific crossing barriers in this genus (Buitendijk et al 1995;de Jeu and Jacobsen 1995;Ishikawa et al 1997) and have been useful for introducing genes from wild species into commercial cultivars. However, some disease and post-harvest problems cannot easily be solved by these methods because the resistance or tolerance factors are not available in the wild species.…”

Section: Introductionmentioning

confidence: 98%

Transformation of the monocotyledonous Alstroemeria by Agrobacterium tumefaciens

2004

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An efficient procedure is described for the transformation of the monocotyledonous Alstroemeria by Agrobacterium tumefaciens via callus regeneration. Calli derived from ovules were co-cultivated with A. tumefaciens strains EHA101 and LBA4404, which harbored the binary vector plasmids pIG121Hm and pTOK233, respectively. These plasmids contain the beta-glucuronidase gene ( gusA) as a reporter gene and the hygromycin phosphotransferase and neomycin phosphotransferase II ( nptII) genes as selective markers. Inoculated calli were first plated for 4 weeks on medium containing cefotaxime to eliminate bacteria, following which time transformed cells were selected on medium that contained 20 mg/l hygromycin. A histochemical assay for GUS activity revealed that hygromycin-based selection was completed after 8 weeks. The integration of the T-DNA of pIG121Hm and pTOK233 into the genome of the cells was confirmed by PCR analysis. Efficient shoot regeneration from the transformed calli was observed on half-strength MS medium supplemented with 0.5 mg/l naphthaleneacetic acid and 0.5 mg/l benzyladenine after about 5 months of culture. The presence of the gusA and nptII genes in the genomic DNA of regenerated plants was detected by means of PCR and PCR-Southern hybridization, and the expression of these transgenes was verified by reverse transcription-PCR.

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“…Therefore various cultivars have been produced. Some interspecific hybrids of Alstroemeria species have been produced using ovule culture (Buitendijk et al, 1995;De Jeu and Jacobsen, 1995;Lu and Bridgen, 1996, Ishikawa et al, 1997, 2001. By conducting detailed studies on interspecific hybridization via ovule culture.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Pre-Fertilization Barriers by Observation of Pollen Tube Growth and Attempts for Overcoming Post-Fertilization Barriers in Intergeneric Hybridization Between Alstroemeria and Bomarea by Ovule Culture

Kashihara¹,

Hirano²,

Murata³

et al. 2010

Acta Hortic.

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In the flower market there is a continuous demand for new cultivars of Alstroemeria as an ornamental flower. Many interspecific hybrids of Alstroemeria are utilized for commercial cultivars. However there is no report about intergeneric hybridization between Alstroemeria and Bomarea. We are investigating the possibility of creating intergeneric hybrids between Alstroemeria and Bomarea. Moreover, ovule culture conditions were examined for overcoming post-fertilization barriers after pollination of Bomarea pollen grains to A. aurea, A. pelegrina var. rosea and A. magenta. The frequency of pollen tube entry into ovules was compared in the pistil of A. aurea, A. pelegrina var. rosea and A. magenta after pollination of B. coccinea pollen grains. Pollen tubes in Alstroemeria ovaries were observed with aniline blue staining 48 hours after pollination. The frequencies of pollen tube entry into ovules were 0.3%, 5.6% and 10.0% in A. aurea, A. pelegrina var. rosea and A. magenta, respectively. For ovule culture, pollinated ovaries were harvested 3 and 7 days after pollination, and the ovules were cultured on 2 g l -1 gellan gum-solidified MS medium with or without gibberellic acid, and supplemented with sucrose at different concentrations (30, 60, 80 or 100 g l -1 ). As a result, 3 plantlets were obtained in A. pelegrina var. rosea × B. coccinea cultured on MS medium supplemented with 80 g l -1 sucrose. Although pollen tubes of B. coccinea were reached to A. aurea ovules, this combination might have stronger pre-fertilization barriers than those of A. pelegrina var. rosea and A. magenta. It supposed to be different intensity of pre-fertilization barriers among species. Our data also suggested that sucrose concentration at 80 g l -1 in culture medium was effective to obtain progenies after intergeneric pollination. Confirmation of hybrid natures for the plantlets is now in progress.

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Early postfertilization ovule culture in Alstroemeria L. and barriers to interspecific hybridization

Cited by 35 publications

References 11 publications

Homoeologous chromosome pairing in the distant hybrid Alstroemeria aurea × A. inodora and the genome composition of its backcross derivatives determined by fluorescence in situ hybridization with species-specific probes

Homoeologous chromosome pairing in the distant hybrid Alstroemeria aurea × A. inodora and the genome composition of its backcross derivatives determined by fluorescence in situ hybridization with species-specific probes

Transformation of the monocotyledonous Alstroemeria by Agrobacterium tumefaciens

Evaluation of Pre-Fertilization Barriers by Observation of Pollen Tube Growth and Attempts for Overcoming Post-Fertilization Barriers in Intergeneric Hybridization Between Alstroemeria and Bomarea by Ovule Culture

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