Transformation of the monocotyledonous Alstroemeria by Agrobacterium tumefaciens

Akutsu, Masako; Ishizaki, Takuma; Sato, Hiroji

doi:10.1007/s00299-003-0729-z

Cited by 23 publications

(11 citation statements)

References 29 publications

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“…Monocotyledonous plants, in particular cereal plants, are naturally outside of the host range of A. tumefaciens, however, so the development of a system for the genetic transformation of monocotyledons has been slow (Hiei et al 1997). Nonetheless, successful transformations has been reported in some monocotyledonous plants, including Oryza (Hiei et al 1994), Hordeum (Tingay et al 1997), Humulus (Horlemann et al 2003) and Alstroemeria (Akutsu et al 2004). The bombardment of cells and tissues, another option for genetic improvement, has been attempted in many plant species (Iida et al 1991;Christou et al 1992;Batista et al 2008).…”

Section: Introductionmentioning

confidence: 94%

Effects of plant growth regulators and l-glutamic acid on shoot organogenesis in the halophyte Leymus chinensis (Trin.)

Sun

Hong

2009

Plant Cell Tiss Organ Cult

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The halophyte Leymus chinensis (Trin.) is a perennial rhizome grass (tribe Gramineae) that is widely distributed in China, Mongolia and Siberia, where it is produced as a forage product. In this report, we establish a highly reproducible plant regeneration system through somatic embryogenesis. Two explants, mature seeds and leaf base segments were used; these parts displayed different responses to combinations of growth factors that affect embryogenic callus induction, callus type optimization and plant regeneration. The highest callus induction frequency was obtained on Murashige and Skoog (MS) medium supplemented with 2.0 mg l -1 2,4-dichlorophenoxyacetic acid (2,4-D) in the presence of 5.0 mg l -1 L-glutamic acid. The inclusion of 5.0 mg l -1 L-glutamic acid was found to significantly promote primary callus induction, embryogenic callus formation and callus status improvement. Subculturing on maintenance medium for 1-2 months before plant regeneration was found to be essential for the optimization of callus type and the maturation of embryogenic callus. Callus relative water content and growth rate were simultaneously investigated during callus maintenance, and found to possibly be related to callus type. Shoots were differentiated from the embryogenic callus on the optimal medium with MS salts containing 0.2-0.5 mg l -1 a-naphthalene acetic acid (NAA), 2.0 mg l -1 kinetin (Kn) and 2.0 g l -1 casamino acids in 71.0 and 69.2% of wild-type (WT) and Jisheng No.1 (JS) plants, respectively. Plant regeneration was variable depending on NAA levels, and the addition of casamino acids stimulated the maturation of embryogenic callus and plant regeneration. Transferring callus with shoots onto half-strength MS medium resulted in rooting within 1 week. The growth of regenerated plants was also surveyed in the field. This is the first report of plant regeneration through somatic embryogenesis from mature seeds and leaf base segments of L. chinensis.

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Section: Introductionmentioning

confidence: 94%

Effects of plant growth regulators and l-glutamic acid on shoot organogenesis in the halophyte Leymus chinensis (Trin.)

Sun

Hong

2009

Plant Cell Tiss Organ Cult

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“…The sequence information can be used to isolate cDNA clones, and these can be used for plant transformation studies to modify gene expression levels. Although Agrobacterium-and/or particle bombardment-mediated transformation techniques are technically challenging in monocot plants, they have been successfully applied in some monocots including Phalaenopsis, Agapanthus, Muscari, Alstroemeria, and Tricyrtis (Adachi et al, 2005;Akutsu et al, 2004;Mishiba et al, 2005;Suzuki and Nakano, 2002;Suzuki et al, 2001). These monocot species can be used for studies to modify floral organ development by overexpression or reduction of B-and C-class floral organ identity genes.…”

Section: Future Prospectsmentioning

confidence: 99%

Molecular Mechanism Regulating Floral Architecture in Monocotyledonous Ornamental Plants

Kanno

2016

The Hortic J

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Molecular and genetic analyses of flower development have been conducted primarily in dicot model plants such as Arabidopsis thaliana and Antirrhinum majus. The obtained data are the basis for the ABC model, which was extended to the ABCE model of floral development. This model has been validated in many dicot species using genetic transformation studies and mutant analyses. Many dicot flowers have two distinctive perianth whorls, which include greenish sepals and showy petals. By contrast, the monocot lily flower has two almost identical petaloid whorls, the inner and outer tepals. To explain this type of floral morphology, a modified ABC model, the further extended modified ABCE model, was proposed. According to this model, B-class genes are expressed in whorls 1-3, and whorls 1 and 2 form petaloid structures. This review describes the molecular mechanisms regulating flower development in monocots. Since the showy perianth is one of the most important traits in floricultural crops, we focused on the B-and C-class genes, which are related to the development and modification of the perianth. The review describes the expression patterns of floral organ identity genes, and presents functional studies using double-flowered and viridiflora cultivars in some monocot species. Besides lily-type flowers, there are several types of monocot flower, such as commelina type with two whorls of distinctive perianth, orchid and grass flowers. The review also describes the molecular analyses of these types of monocot flower.

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“…Because Agrobacterium-mediated transformation does not require the use of protoplasts, and the T-DNA is stably integrated into genomic DNA in low copy number, it is preferred by many breeders using formerly protoplasts or particle bombardment technique. Although monocots are generally considered to be outside the host range of A. tumefaciens (De Cleene and Deley 1976), A. tumefaciens-mediated transformation has been reported in Gramineae such as Festuca arundinacea (Bettany et al 2003), buffel (Batra and Kumar 2003), bentgrass , Alstroemeria (Akutsu et al 2004), typha (Nandakumar et al 2005) and E. sibiricus cv. 'chuancao No.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium–mediated genetic transformation of Elymus breviaristatus with Pseudomonas pseudoalcaligenes insecticidal protein gene

Li¹,

Sun

Huang

et al. 2007

Plant Cell Tiss Organ Cult

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An efficient and repeatable protocol for generating transgenic Elymus breviaristatus plants was first established by Agrobacterium-mediated transformation: calli of Elymus breviaristatus were induced from mature seeds, and proliferated on callus induction and maintenance medium containing kinetin 0.05 mg/l and 2, 4-D 8.0 mg/l under dim-light condition (10-20 mmol/m 2 s,16 h light) at 268C. The calli produced were used for transformation mediated by Agrobacterium tumefaciens, EHA105, which carries the binary vector pCAMBIA 1304-ppip, coding for the Pseudomonas pseudoalcaligenes insecticidal protein gene (ppip) and hygromycin phosphotransferase II gene (hptII). The calli transformed were selected and grown in the presence of 60 mg/l hygromycin. Approximately 90 transgenic plants were produced, and transformation frequency of the calli reached 10.52%. The presence of ppip gene in the genomic DNA of regenerated plants was detected by means of PCR and Southern-blot analysis, and the expression of the transgenes was verified by reverse transcription-PCR. Approximately 90% of the tested plants appeared to have only one or two copies of the T-DNA inserts. Verification of anti-grasshoppers activity shows the mortality of grasshoppers reached 16.73%. These results indicated that Agrobacteriummediated transformation in our condition was effective for transferring foreign genes into Elymus breviaristatus.

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Transformation of the monocotyledonous Alstroemeria by Agrobacterium tumefaciens

Cited by 23 publications

References 29 publications

Effects of plant growth regulators and l-glutamic acid on shoot organogenesis in the halophyte Leymus chinensis (Trin.)

Effects of plant growth regulators and l-glutamic acid on shoot organogenesis in the halophyte Leymus chinensis (Trin.)

Molecular Mechanism Regulating Floral Architecture in Monocotyledonous Ornamental Plants

Agrobacterium–mediated genetic transformation of Elymus breviaristatus with Pseudomonas pseudoalcaligenes insecticidal protein gene

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