Early induction of Fe-SOD gene expression is involved in tolerance to Mn toxicity in perennial ryegrass

Ribera-Fonseca, Alejandra; Inostroza‐Blancheteau, Claudio; Cartes, Paula; Rengel, Zed; Mora, Marı́a de la Luz

doi:10.1016/j.plaphy.2013.08.012

Cited by 49 publications

(27 citation statements)

References 37 publications

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“…Total soluble phenols were spectrophotometrically assayed at 765 nm using Folin-Ciocalteu reagent according to the method described by Slinkard and Singleton (1977) with minor modifications (Ribera et al, 2013). Total phenol concentration was calculated using chlorogenic acid as a phenolic compound standard.…”

Section: Methodsmentioning

confidence: 99%

“…The primer sets used for LpLsi1 (GenBank accession number KY315994) and LpLsi2 (GenBank accession number KY315995) were designed using the Primer3 (v. 0.4.0) and primer BLAST tools. Primers sequences for LpCu/ZnSOD, LpFe-SOD , and LpMn-SOD were obtained from Ribera et al (2013). Housekeeping genes, LpActin or LpeEF1A (m) , were used as internal controls (Ribera et al, 2013).…”

Section: Methodsmentioning

confidence: 99%

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Silicon-Mediated Alleviation of Aluminum Toxicity by Modulation of Al/Si Uptake and Antioxidant Performance in Ryegrass Plants

et al. 2017

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Silicon (Si) has been well documented to alleviate aluminum (Al) toxicity in vascular plants. However, the mechanisms underlying these responses remain poorly understood. Here, we assessed the effect of Si on the modulation of Si/Al uptake and the antioxidant performance of ryegrass plants hydroponically cultivated with Al (0 and 0.2 mM) in combination with Si (0, 0.5, and 2.0 mM). Exposure to Al significantly increased Al concentration, mainly in the roots, with a consequent reduction in root growth. However, Si applied to the culture media steadily diminished the Al concentration in ryegrass, which was accompanied by an enhancement in root dry matter production. A reduced concentration of Si in plant tissues was also observed when plants were simultaneously supplied with Al and Si. Interestingly, Si transporter genes (Lsi1 and Lsi2) were down-regulated in roots after Si or Al was applied alone; however, both Lsi1 and Lsi2 were up-regulated as a consequence of Si application to Al-treated plants, denoting that there is an increase in Si requirement in order to cope with Al stress in ryegrass. Whereas Al addition triggered lipid peroxidation, Si contributed to an attenuation of Al-induced oxidative stress by increasing phenols concentration and modulating the activities of superoxide dismutase (SOD), catalase, peroxidase, and ascorbate peroxidase antioxidant enzymes. Differential changes in gene expression of SOD isoforms (Mn-SOD, Cu/Zn-SOD, and Fe-SOD) and the profile of peroxide (H2O2) generation were also induced by Si in Al-stressed plants. This, to the best of our knowledge, is the first study to present biochemical and molecular evidence supporting the effect of Si on the alleviation of Al toxicity in ryegrass plants.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Silicon-Mediated Alleviation of Aluminum Toxicity by Modulation of Al/Si Uptake and Antioxidant Performance in Ryegrass Plants

et al. 2017

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“…In this regard, forage grasses whose contents of Cu and Zn are reduced when cultivated in the presence of toxic elements like Cd and Pb may have their growth considerably limited and be inefficient if used in the process of phytoremediation of environments with extremely high contents of heavy metals. It should be mentioned that Cu and Zn are components of metalloenzymes 6 and are involved in oxidation-reduction processes, in detoxification of O 2 -, and in protein and lignin syntheses, i.e., in processes essential to plants exposed to heavy metals (MARSCHNER, 2012;RIBERA-FONSECA et al, 2013). Regarding Fe, the appropriate content range for forage grasses (50-250mg kg -1 dry matter) is wider than the ranges of Cu and Zn, but a decrease in the content of this nutrient affects the photosynthesis (WERNER et al, 1996;MARSCHNER, 2012).…”

Section: Relationship Between Heavy Metals and Cationic Micronutrientmentioning

confidence: 99%

“…As in the case of SOD, there are also isoforms of CAT, APX, and GPX (GILL & TUTEJA, 2010). Transcription of genes that code SOD isoforms in Lolium perenne plants increased when they were exposed to Pb (LI et al, 2012) and to Mn (RIBERA-FONSECA et al, 2013). However, in Panicum maximum grown with Cd, no alterations were identified in SOD and CAT isoforms (RABÊLO, 2014).…”

Section: Relationship Between Heavy Metals and Cationic Micronutrientmentioning

confidence: 99%

Changes caused by heavy metals in micronutrient content and antioxidant system of forage grasses used for phytoremediation: an overview

Rabêlo

Borgo

2016

Cienc. Rural

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An increase in the content of heavy metals in the environment causes many socio-environmental problems, and phytoremediation is a tool to reduce the environmental impact caused by these elements, with prospects for the use of forage grasses. This group of plants features characteristics for the environment-decontamination process, but further studies are necessary about the damages caused by heavy metals on the uptake of cationic micronutrients and on the antioxidant system, which are essential processes for the growth of plants in contaminated sites. Exposure of forage grasses to heavy metals results in a lower content of Mn in the shoots of almost all plants, but the contents of Cu, Fe, and Zn vary according to heavy metal and forage grass. Activities of enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), and guaiacol peroxidase (GPX) usually increase to reduce the oxidative stress induced by heavy metals, but when the content of any of these metals is high, enzymatic activity is decreased. Scale of toxicity of heavy metals to forage grasses can be described as: Pb ≈ Cr > Cd ≈ As > Zn ≈ Cu ≈ Ni > Mn.

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“…SODs are metal-containing enzymes which comprise several isoforms that can be distinguished on the basis of their metal cofactor. In general, plants contain a mitochondrial MnSOD, as well as a cytosolic and a chloroplastic Cu/Zn-SOD; also, many plants contain chloroplast Fe-SOD [5,9] .…”

Section: Cyclingmentioning

confidence: 99%

Cadmium-induced oxidative damage and antioxidant defense mechanisms in Glycine max L.

2014

JCLM

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Early induction of Fe-SOD gene expression is involved in tolerance to Mn toxicity in perennial ryegrass

Cited by 49 publications

References 37 publications

Silicon-Mediated Alleviation of Aluminum Toxicity by Modulation of Al/Si Uptake and Antioxidant Performance in Ryegrass Plants

Silicon-Mediated Alleviation of Aluminum Toxicity by Modulation of Al/Si Uptake and Antioxidant Performance in Ryegrass Plants

Changes caused by heavy metals in micronutrient content and antioxidant system of forage grasses used for phytoremediation: an overview

Cadmium-induced oxidative damage and antioxidant defense mechanisms in Glycine max L.

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