Early hemorheological changes in a porcine model of intravenously given E. coli induced fulminant sepsis

Németh, Norbert; Berhés, Mariann; Kiss, Ferenc; Hajdú, Endre; Deák, Ádám; Molnár, Ábel; Szabó, Judit; Fülesdi, Béla

doi:10.3233/ch-141914

Cited by 14 publications

(9 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Changes in the mechanical properties of erythrocytes during sepsis, which are manifested by increased RBC rigidity and measured by decreased RBC deformability, are clinically relevant for a number of reasons. First, decreased erythrocyte deformability develops early in rat (3–6 h) and pig (6–8 h) models of sepsis [ 3 , 56 , 57 ] and has been detected within 24 h of admission of critically ill patients [ 30 , 32 ]. Second, it may provide a means to differentiate between trauma and septic patients [ 30 ], as RBC deformability recovered in the former but not in the latter group of patients.…”

Section: Erythrocyte Size Shape and Deformabilitymentioning

confidence: 99%

The Effect of Sepsis on the Erythrocyte

Bateman

Sharpe

Singer

et al. 2017

IJMS

125

131

View full text Add to dashboard Cite

Sepsis induces a wide range of effects on the red blood cell (RBC). Some of the effects including altered metabolism and decreased 2,3-bisphosphoglycerate are preventable with appropriate treatment, whereas others, including decreased erythrocyte deformability and redistribution of membrane phospholipids, appear to be permanent, and factors in RBC clearance. Here, we review the effects of sepsis on the erythrocyte, including changes in RBC volume, metabolism and hemoglobin’s affinity for oxygen, morphology, RBC deformability (an early indicator of sepsis), antioxidant status, intracellular Ca2+ homeostasis, membrane proteins, membrane phospholipid redistribution, clearance and RBC O2-dependent adenosine triphosphate efflux (an RBC hypoxia signaling mechanism involved in microvascular autoregulation). We also consider the causes of these effects by host mediated oxidant stress and bacterial virulence factors. Additionally, we consider the altered erythrocyte microenvironment due to sepsis induced microvascular dysregulation and speculate on the possible effects of RBC autoxidation. In future, a better understanding of the mechanisms involved in sepsis induced erythrocyte pathophysiology and clearance may guide improved sepsis treatments. Evidence that small molecule antioxidants protect the erythrocyte from loss of deformability, and more importantly improve septic patient outcome suggest further research in this area is warranted. While not generally considered a critical factor in sepsis, erythrocytes (and especially a smaller subpopulation) appear to be highly susceptible to sepsis induced injury, provide an early warning signal of sepsis and are a factor in the microvascular dysfunction that has been associated with organ dysfunction.

show abstract

Section: Erythrocyte Size Shape and Deformabilitymentioning

confidence: 99%

The Effect of Sepsis on the Erythrocyte

Bateman

Sharpe

Singer

et al. 2017

IJMS

125

131

View full text Add to dashboard Cite

show abstract

“…In the E.coli-treated group, sepsis was induced by Escherichia coli suspension (2.5 × 10 5 /mL; strain: ATCC 25922, Department of Medical Microbiology, University of Debrecen), which was intravenously administered in a continuously increasing manner: 2 mL in the first 30 minutes, then 4 mL in 30 minutes and afterwards 16 mL/hour for 2 hours. A total amount of 9.5 × 10 6 E. coli was infused within 3 hours [8,9]. In the control group the similar volume of isotonic saline solution was administered by the same protocol as in the septic group and no other intervention was applied.…”

Section: Porcine Sepsis Modelmentioning

confidence: 99%

Red blood cell and platelet parameters are sepsis predictors in an Escherichia coli induced lethal porcine model

Tóth

Debreceni

Berhés

et al. 2017

View full text Add to dashboard Cite

show abstract

“…There are several limitations of the present study: We used a porcine model of intravenously administered E.coli suspension [20, 33]. Although this model is regarded as an endotoxicosis model rather than a classical sepsis model, it is widely accepted that it is suitable for modeling extreme clinical sepsis, such us meningococcaemia or gram-negative bacteremia in cases of granulocytopenia [34] .…”

Section: Discussionmentioning

confidence: 99%

Assessment of cerebral circulation in a porcine model of intravenously given E. coli induced fulminant sepsis

et al. 2017

View full text Add to dashboard Cite

BackgroundThe aim of the present work was to assess cerebral hemodynamic changes in a porcine model of E.coli induced fulminant sepsis.MethodsNineteen healthy female Hungahib pigs, 10–12 weeks old, randomly assigned into two groups: Control (n = 9) or Septic Group (n = 10). In the Sepsis group Escherichia coli culture suspended in physiological saline was intravenously administrated in a continuously increasing manner according to the following protocol: 2 ml of bacterial culture suspended in physiological saline was injected in the first 30 min, then 4 ml of bacterial culture was administered within 30 min, followed by infusion of 32 ml bacterial culture for 2 h. Control animals received identical amount of saline infusion. Systemic hemodynamic parameters were assessed by PiCCo monitoring, and cerebral hemodynamics by transcranial Doppler sonography (transorbital approach) in both groups.Results In control animals, systemic hemodynamic variables and cerebral blood flow velocities and pulsatility indices were relatively stable during the entire procedure. In septic animals shock developed in 165 (IQR: 60–255) minutes after starting the injection of E.coli solution. Blood pressure values gradully decreased, whereas pulse rate increased. A decrease in cardiac index, an increased systemic vascular resistance, and an increased stroke volume variation were observed. Mean cerebral blood flow velocity in the middle cerebral artery did not change during the procedure, but pulsatility index significantly increased.ConclusionsThere is vasoconstriction at the level of the cerebral arterioles in the early phase of experimental sepsis that overwhelmes autoregulatory response. These results may serve as additional pathophysiological information on the cerebral hemodynamic changes occurring during the septic process and may contribute to a better understanding of the pathomechanism of septic encephalopathy.

show abstract

Early hemorheological changes in a porcine model of intravenously given E. coli induced fulminant sepsis

Cited by 14 publications

References 34 publications

The Effect of Sepsis on the Erythrocyte

The Effect of Sepsis on the Erythrocyte

Red blood cell and platelet parameters are sepsis predictors in an Escherichia coli induced lethal porcine model

Assessment of cerebral circulation in a porcine model of intravenously given E. coli induced fulminant sepsis

Contact Info

Product

Resources

About