2007
DOI: 10.1677/jme-06-0069
|View full text |Cite
|
Sign up to set email alerts
|

Early growth response gene-1 plays a pivotal role in down-regulation of a cohort of genes in uterine leiomyoma

Abstract: Microarray studies have identified many genes that are down-regulated in uterine leiomyoma compared with myometrium, including early growth response gene-1 (EGR1). However, the mechanisms underlying coordinated down-regulation of this gene cohort remain unknown. To address the transcriptional role of EGR1 in leiomyoma, EGR1 binding to promoter sequences on target genes was assessed by chromatin immunoprecipitation (ChIP) assay in leiomyoma tissues and myometrium-derived KW cells. Computer analysis demonstrated… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

1
8
0

Year Published

2008
2008
2017
2017

Publication Types

Select...
7

Relationship

0
7

Authors

Journals

citations
Cited by 12 publications
(9 citation statements)
references
References 29 publications
1
8
0
Order By: Relevance
“…2 E ). In agreement with these observations, we found the lung metastasis signature to contain 22% (12/54) of the genes predicted elsewhere to be EGR1 transcriptional targets [31][34] and the wound response was previously shown to be coordinated with MYC amplification [35]. In addition, over-representation of an AHR motif is consistent with its association with ER to regulate cell proliferation [36].…”
Section: Resultssupporting
confidence: 85%
“…2 E ). In agreement with these observations, we found the lung metastasis signature to contain 22% (12/54) of the genes predicted elsewhere to be EGR1 transcriptional targets [31][34] and the wound response was previously shown to be coordinated with MYC amplification [35]. In addition, over-representation of an AHR motif is consistent with its association with ER to regulate cell proliferation [36].…”
Section: Resultssupporting
confidence: 85%
“…TGFβ is known to be dysregulated i.e. increased, in leiomyoma relative to myometrium (39, 40). This increase, in part, could also explain the downregulation of miR-29 seen in leiomyoma.…”
Section: Discussionmentioning
confidence: 99%
“…The sheared DNA size was from 600 to 900 bp, with the majority around 800 bp (data not shown). The Egr1 antibody from Santa Cruz Biotechnology was chosen because it has been successfully used in ChIP assays previously (35). Immunoprecipitation of antibody-chromatin complex was performed with protein G-agarose provided in the kit.…”
Section: Methodsmentioning
confidence: 99%