Early gene Broad complex plays a key role in regulating the immune response triggered by ecdysone in the Malpighian tubules of Drosophila melanogaster

Verma, Puja; Tapadia, Madhu G.

doi:10.1016/j.molimm.2015.03.249

Cited by 27 publications

(38 citation statements)

References 49 publications

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“…We suggest this possibility since several studies in Drosophila and other insects have shown that the developmental hormones ecdysone and juvenile hormone regulate the expression of a number of genes involved in innate immune responses2526272829. Interestingly, one of these studies showed that in the Drosophila Malpighian tubules of both larvae and adults, AMPs respond to ecdysone very quickly in the absence of infection28.…”

Section: Discussionmentioning

confidence: 95%

“…Interestingly, one of these studies showed that in the Drosophila Malpighian tubules of both larvae and adults, AMPs respond to ecdysone very quickly in the absence of infection28. In a previous study, these authors showed constitutive expression of AMPs in the Malpighian tubules that persisted from larvae to adults independent of infection30.…”

Section: Discussionmentioning

confidence: 98%

“…It is possible that we are seeing a similar hormonal effect on TmCactin expression, perhaps resulting from either ecdysone or another developmental hormone, in the Malpighian tubules. Considering the above reasons, and given that components of the IMD32 and Toll33 pathways, and AMPs2834 are expressed in the Drosophila Malpighian tubules, it is not surprising that TmCactin is highly expressed in these tissues of T. molitor larvae and adults. However, it is less clear why TmCactin is highly expressed in the larvae and adult integuments.…”

Section: Discussionmentioning

confidence: 99%

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TmCactin plays an important role in Gram-negative and -positive bacterial infection by regulating expression of 7 AMP genes in Tenebrio molitor

Kim

Park

et al. 2017

Sci Rep

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Cactin was originally identified as an interactor of the Drosophila IκB factor Cactus and shown to play a role in controlling embryonic polarity and regulating the NF-κB signaling pathway. While subsequent studies have identified the roles for Cactin in the mammalian immune response, the immune function of Cactin in insects has not been described yet. Here, we identified a Cactin gene from the mealworm beetle, Tenebrio molitor (TmCactin) and characterized its functional role in innate immunity. TmCactin was highly expressed in prepupa to last instar stages, and its expression was high in the integument and Malpighian tubules of last instar larvae and adults. TmCactin was induced in larvae after infection with different pathogens and detectable within 3 hours of infection. The highest levels of TmCactin expression were detected at 9 hours post infection. TmCactin RNAi significantly decreased the survival rates of larvae after challenge with Escherichia coli and Staphylococcus aureus, but had no significant effect after challenge with Candida albicans. Furthermore, TmCactin RNAi significantly reduced the expression of seven antimicrobial peptide genes (AMPs) after bacterial challenge. Our results suggest that TmCactin may serve as an important regulator of innate immunity, mediating AMP responses against both Gram-positive and Gram-negative bacteria in T. molitor.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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TmCactin plays an important role in Gram-negative and -positive bacterial infection by regulating expression of 7 AMP genes in Tenebrio molitor

Kim

Park

et al. 2017

Sci Rep

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“…In addition, silencing BrZ2 resulted in tree‐top disease, as well as higher levels of virus replication, OB production and faster time to death, indicating that BrZ2 is a negative regulator of HaSNPV‐induced climbing behaviour and acts downstream of the 20E and JH signalling pathways. In D. melanogaster , the Br‐C gene was found to play a key role in regulating the immune response triggered by ecdysone in the Malpighian tubules (Verma & Tapadia, ).…”

Section: Discussionmentioning

confidence: 99%

Host miRNAs are involved in hormonal regulation of HaSNPV‐triggered climbing behaviour in Helicoverpa armigera

Zhang

Hoover

et al. 2018

Molecular Ecology

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Baculoviruses manipulate host climbing behaviour to ensure that the hosts die at elevated positions on host plants to facilitate virus proliferation and transmission, which is a process referred to as tree-top disease. However, the detailed molecular mechanism underlying tree-top disease has not been elucidated. Using transcriptome analysis, we showed that two hormone signals, juvenile hormone (JH) and 20-hydroxyecdysone (20E), are key components involved in HaSNPV-induced tree-top disease in Helicoverpa armigera larvae. RNAi-mediated knockdown and exogenous hormone treatment assays demonstrated that 20E inhibits virus-induced tree-top disease, while JH mediates tree-top disease behaviour. Knockdown of BrZ2, a downstream signal of JH and 20E, promoted HaSNPV-induced tree-top disease. We also found that two miRNAs target BrZ2 and are involved in the cross-talk regulation between 20E and JH manipulating HaSNPV replication, time to death and HaSNPV-induced tree-top disease.

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“…The most important discoveries to illustrate this progress have been the isolation and sequencing of diuretic and antidiuretic peptides, the discovery of the V-type H + -ATPase as an energizer of plasma membranes, and the studies of epithelial transport functions using modern experimental methods. 11,27 Genomic and proteomic analysis studies may elucidate their intracellular signaling mechanisms, 81,82 regulating the immune response, 83 in addition to other functions of MTs, as their relationship with the bioluminescence mechanism in some insects. 84,85 The results indicate that MTs can be used for the evaluation of biomarkers, at the cellular level, in toxicological studies, especially studies done with sublethal doses and concentrations of chemicals.…”

Section: Closing Argumentsmentioning

confidence: 99%

Comparative physiology of Malpighian tubules: form and function

Cintra-Socolowski¹,

Nocelli²,

Roat³

et al. 2016

OAIP

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Early gene Broad complex plays a key role in regulating the immune response triggered by ecdysone in the Malpighian tubules of Drosophila melanogaster

Cited by 27 publications

References 49 publications

TmCactin plays an important role in Gram-negative and -positive bacterial infection by regulating expression of 7 AMP genes in Tenebrio molitor

TmCactin plays an important role in Gram-negative and -positive bacterial infection by regulating expression of 7 AMP genes in Tenebrio molitor

Host miRNAs are involved in hormonal regulation of HaSNPV‐triggered climbing behaviour in Helicoverpa armigera

Comparative physiology of Malpighian tubules: form and function

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