Early detection of metallo-β-lactamase NDM-1- and OXA-23 carbapenemase-producing Acinetobacter baumannii in Libyan hospitals

Mathlouthi, Najla; Salabi, Allaaeddin Ali El; Jomaa-Jemili, Ă Mariem Ben; Bakour, Sofiane; Al-Bayssari, Charbel; Zorgani, Abdulaziz; Kraiema, Abdulmajeed; Elahmer, Omar; Okdah, Liliane; Rolain, Jean‐Marc; Chouchani, Chedly

doi:10.1016/j.ijantimicag.2016.03.007

Cited by 48 publications

(23 citation statements)

References 29 publications

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“…The bla NDM-1 carrying A. baumannii has recently been emerged in many countries, including Germany, Spain, Israel, Egypt, Switzerland, Libya, India, Pakistan and Nepal [ 11 , 26 , 32 , 33 ]. The bla NDM-1 gene has been identified as a chimeric gene constructed by the fusion of the aminoglycoside-resistance gene aphA6 with a mannose-binding lectin gene.…”

Section: Discussionmentioning

confidence: 99%

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Co-existence of bla OXA-23 and bla NDM-1 genes of Acinetobacter baumannii isolated from Nepal: antimicrobial resistance and clinical significance

Joshi

Acharya

Kakshapati

et al. 2017

Antimicrob Resist Infect Control

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BackgroundMolecular analysis of carbapenem-resistant genes in Acinetobacter baumannii, an emerging pathogen, is less commonly reported from Nepal. In this study we determined the antibiotic susceptibility profile and genetic mechanism of carbapenem resistance in clinical isolates of A. baumannii. Methods A. baumannii were isolated from various clinical specimens and identified based on Gram staining, biochemical tests, and PCR amplification of organism specific 16S rRNA and bla OXA-51 genes. The antibiotic susceptibility testing was performed using disc diffusion and E-test method. Multiplex PCR assays were used to detect the following β-lactamase genes: four class D carbapenem hydrolyzing oxacillinases (bla OXA-51, bla OXA-23, bla OXA-24 and bla OXA-58). Uniplex PCRs were used to detect three class B metallo-β-lactamases genes (bla IMP, bla VIM and bla NDM-1), class C cephalosporin resistance genes (bla ADC), aminoglycoside resistance gene (aphA6), and ISAba1 of all isolates. Insertion sequence ISAba125 among NDM-1 positive strains was detected. Clonal relatedness of all isolates were analyzed using repetitive sequence-based PCR (rep-PCR).ResultsOf total 44 analyzed isolates, 97.7% (n = 43) were carbapenem-resistant A. baumannii (CR-AB) and 97.7% (n = 43) were multidrug resistant A. baumannii (MDR-AB). One isolate was detected to be extremely drug resistant A. baumannii (XDR-AB). All the isolates were fully susceptible to colistin (MICs < 2 μg/ml). The bla OXA-23 gene was detected in all isolates, while bla NDM-1 was detected in 6 isolates (13.6%). Insertion sequence, ISAba1 was detected in all of bla OXA-23 positive isolates. ISAba125 was detected in all bla NDM-1 positive strains. The bla ADC and aphA6 genes were detected in 90.1 and 40.1%, respectively. The rep-PCR of all isolates represented 7 different genotypes.ConclusionWe found high prevalence of CR-AB and MDR-AB with bla OXA-23 gene in a tertiary care hospital in Nepal. Systemic network surveillance should be established for monitoring and controlling the spread of these resistant strains.Electronic supplementary materialThe online version of this article (doi:10.1186/s13756-017-0180-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

“…The previous study reported that the most of A. baumannii isolates harboring bla NDM-1 belonged to ST85 and ST25 [ 35 – 37 ]. In Libyan hospital, Libya, the main clone of imipenem-resistant NDM-1-producing A. baumannii belonged to ST2 [ 33 ]. We used rep-PCR typing to determine the clonal relationship in NDM-1 producing A. baumannii .…”

Section: Discussionmentioning

confidence: 99%

Co-existence of bla OXA-23 and bla NDM-1 genes of Acinetobacter baumannii isolated from Nepal: antimicrobial resistance and clinical significance

Joshi

Acharya

Kakshapati

et al. 2017

Antimicrob Resist Infect Control

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“… [ 7 , 50 ] K. pneumoniae KPC-3 Italy [ 51 ] A. baumanii n.r. Libya [ 52 ] A. baumanii OXA-23 like; NDM-1 Morocco [ 19 ] A.baumanii n.r. Mongolia [ 53 ] A. baumanii OXA-58 P. aeruginosa VIM-2 Pakistan [ 28 , 54 ] K. pneumoniae OXA-48&NDM; OXA-48 [ 28 ] P. stuartii NDM P. aeruginosa VIM K. oxytoca NDM E. coli OXA-48&NDM A. baumanii OXA 23 Poland [ 55 ] A. baumanii OXA-23 like; OXA-40 like Romania a This study, [ 28 , 56 ] A. baumanii OXA-40; OXA-23 [ 28 ] E. coli OXA-48 [ 56 ] P. aeruginosa n.r.…”

Section: Resultsmentioning

confidence: 99%

Extensive colonization with carbapenemase-producing microorganisms in Romanian burn patients: infectious consequences from the Colectiv fire disaster

Pirii

Friedrich

Rossen

et al. 2017

Eur J Clin Microbiol Infect Dis

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Health care of severe burn patients is highly specialized and may require international patient transfer. Burn patients have an increased risk of developing infections. Patients that have been hospitalized in countries where carbapenemase-producing microorganisms (CPMO) are endemic may develop infections that are difficult to treat. In addition, there is a risk on outbreaks with CPMOs in burn centers. This study underlines that burn patients may extensively be colonized with CPMOs, and it provides best practice recommendations regarding clinical microbiology and infection control. We evaluated CPMO-carriage and wound colonization in a burn patient initially treated in Romania, and transported to the Netherlands. The sequence types and acquired beta-lactamase genes of highly-resistant microorganisms were derived from next generation sequencing data. Next, we searched literature for reports on CPMOs in burn patients. Five different carbapenemase-producing isolates were cultured: two unrelated OXA-48-producing Klebsiella pneumoniae isolates, OXA-23-producing Acinetobacter baumanii, OXA-48-producing Enterobacter cloacae, and NDM-1-producing Providencia stuartii. Also, multi-drug resistant Pseudomonas aeruginosa isolates were detected. Among the sampling sites, there was high variety in CPMOs. We found 46 reports on CPMOs in burn patients. We listed the epidemiology of CPMOs by country of initial treatment, and summarized recommendations for care of these patients based on these reports and our study.

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“…In the last decade, several classes of -lactamases have been detected in A. baumannii [24,25]. The carbapenemases found are mostly belonging to the carbapenem-hydrolyzing OXA-type class D ß-lactamase (CHDL) [26].…”

Section: Discussionmentioning

confidence: 99%

Effect of Antibiotic Combinations on the Sensitivity of Carbapenem Resistant Acinetobacter baumannii Strains

Elhabibi¹,

Ramzy²

2017

J Microb Biochem Technol

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Early detection of metallo-β-lactamase NDM-1- and OXA-23 carbapenemase-producing Acinetobacter baumannii in Libyan hospitals

Cited by 48 publications

References 29 publications

Co-existence of bla OXA-23 and bla NDM-1 genes of Acinetobacter baumannii isolated from Nepal: antimicrobial resistance and clinical significance

Co-existence of bla OXA-23 and bla NDM-1 genes of Acinetobacter baumannii isolated from Nepal: antimicrobial resistance and clinical significance

Extensive colonization with carbapenemase-producing microorganisms in Romanian burn patients: infectious consequences from the Colectiv fire disaster

Effect of Antibiotic Combinations on the Sensitivity of Carbapenem Resistant Acinetobacter baumannii Strains

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