Co-existence of bla OXA-23 and bla NDM-1 genes of Acinetobacter baumannii isolated from Nepal: antimicrobial resistance and clinical significance

Joshi, Pusp Raj; Acharya, Mahesh; Kakshapati, Trishna; Leungtongkam, Udomluk; Thummeepak, Rapee; Sitthisak, Sutthirat

doi:10.1186/s13756-017-0180-5

Cited by 68 publications

(79 citation statements)

References 35 publications

(41 reference statements)

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“…Coexistence of bla NDM-1 and bla OXA-48 carrying plasmids was reported in an isolate of K. pneumoniae from China [72]. Similarly, coexistence of bla NDM-1 and bla OXA-23 was reported in A. baumannii from Nepal [73]. K. pneumoniae of type 11 was reported in Greek to be coproducing two metallo beta-lactamses markers, NDM-1 and VIM-1 together [74].…”

Section: Introductionmentioning

confidence: 97%

Structure, Genetics and Worldwide Spread of New Delhi Metallo-β-lactamase (NDM): a threat to public health

Khan

Maryam

Zarrilli³

2017

BMC Microbiol

413

295

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BackgroundThe emergence of carbapenemase producing bacteria, especially New Delhi metallo-β-lactamase (NDM-1) and its variants, worldwide, has raised amajor public health concern. NDM-1 hydrolyzes a wide range of β-lactam antibiotics, including carbapenems, which are the last resort of antibiotics for the treatment of infections caused by resistant strain of bacteria.Main bodyIn this review, we have discussed bla NDM-1variants, its genetic analysis including type of specific mutation, origin of country and spread among several type of bacterial species. Wide members of enterobacteriaceae, most commonly Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, and gram-negative non-fermenters Pseudomonas spp. and Acinetobacter baumannii were found to carry these markers. Moreover, at least seventeen variants of bla NDM-type gene differing into one or two residues of amino acids at distinct positions have been reported so far among different species of bacteria from different countries. The genetic and structural studies of these variants are important to understand the mechanism of antibiotic hydrolysis as well as to design new molecules with inhibitory activity against antibiotics.ConclusionThis review provides a comprehensive view of structural differences among NDM-1 variants, which are a driving force behind their spread across the globe.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-017-1012-8) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 97%

Structure, Genetics and Worldwide Spread of New Delhi Metallo-β-lactamase (NDM): a threat to public health

Khan

Maryam

Zarrilli³

2017

BMC Microbiol

413

295

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“…the isolate showing DDST + bla VIM + bla GIM positivity. In an earlier study from Nepal, co-existence of bla OXA-23 and bla NDM-1 was detected [45] with the presence of other class B MBLs, such as bla VIM and bla GIM . One of the recent studies showed the presence of bla VIM only in imipenem-resistant genomic species of 13TU -A. baumannii and not in other isolates [46].…”

Section: Discussionmentioning

confidence: 81%

Prevalence of VIM- and GIM-producing Acinetobacter baumannii from patients with severe urinary tract infection

Girija¹,

Priyadharsini

Arumugam

2018

AMicr

117

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Carbapenems are administered as the final drug of choice for treating complicated nosocomial infections caused by multidrug-resistant Acinetobacter baumannii strains. It is currently a worldwide issue that metallo-β-lactamases (MBLs) as carbapenem-hydrolyzing enzymes are one of the major drug resistance mechanisms. This investigation is thus aimed to assess the prevalence and characterize the MBL-producing strains of A. baumannii both by phenotypic assays and by genotypic characterization. A total of 73 isolates of A. baumannii were phenotypically and genotypically characterized from patients (N = 1,000) with severe urinary tract infection. Tested strains were subjected to double disc synergy testing (DDST) by Kirby-Bauer disc diffusion method with imipenem (IMP) and IMP/EDTA combination discs. Plasmid DNA was molecularly screened for MBL-encoding bla IMP , bla VIM , bla GIM , and bla NDM genes by PCR for the genetic relatedness of the MBL genes with carbapenem resistance. Carbapenem resistance profile showed 100%, 45%, and 49% non-susceptibility against imipenem, doripenem, and meropenem, respectively. Altogether 42.46% (n = 31) of the isolates showed MBL production upon double disc phenotypic test with IMP and IMP/EDTA discs. The bla VIM and bla GIM were detected in 34.24% (n = 25) and 16.43% (n = 12) of the isolates, respectively, while the co-occurrence of bla VIM and bla GIM was 2.73% among the isolates. DDST-positive isolates showed 21.19% and 9.58% strains positive for bla VIM and bla GIM , respectively, whereas 1.36% of the strains for both genes. None of the strains yielded bla IMP and bla NDM genes. The findings of this study showed prevalence of carbapenem resistance among A. baumannii from urine samples and the frequency of bla VIM and bla GIM.

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“…ESBL production might be due to the presence of ESBL producing genes like bla OXA-23 and antibiotic genes that can be transferred to other bacteria horizontally through conjugation and due to excessive use of broad spectrum antibiotics (Joshi et al 2017;Shrestha et al 2017). The acquisition of MBL-encoding genes such as vim1, vim2, imp1 and imp2 is one of the ways to acquire resistance to carbapenems like imipenem, meropenem and ertapenem (Davoodi et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Beta-Lactamases Production in Multi-drug Resistant Acinetobacter species Isolated from Different Clinical Specimens

Neupane¹,

Sudeep²,

Thakur³

et al. 2019

TU J. Microbiol.

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Objectives: To determine the prevalence of Acinetobacter spp. from different clinical specimens and detect different types of β-lactamase enzymes. Methods: Different clinical samples were collected and 125 Acinetobacter spp. were isolated. Various biochemical tests were carried out to speciate the Acinetobacter spp. The antibiotic susceptibility pattern and β-lactamase enzymes like Extended spectrum β-lactamase (ESBL), Metallo β-lactamase (MBL) and AmpC β-lactamase were determined. Results: Of the total 125 isolates, the most predominant species was Acinetobacter calcoaceticus-A. baumannii (Acb) complex (80%). Highest rate of isolation of Acinetobacter species were from in-patients (neonates’ blood sample). Among all, 44.8% isolates were found to be MDR with the majority being resistant to aminoglycosides, carbapenems and fluoroquinolones but not to colistin. ESBL, MBL and AmpC beta-lactamase was detected in 43.2%, 15.2% and 1.6% of the isolates respectively. Conclusion: Acinetobacter calcoaceticus-A. baumannii complex should be considered for detection in hospitalized patients. The analysis of antibiotic susceptibility pattern and β-lactamases would be helpful to establish network surveillance in order to maintain and control the spread of these resistant strains.

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Co-existence of bla OXA-23 and bla NDM-1 genes of Acinetobacter baumannii isolated from Nepal: antimicrobial resistance and clinical significance

Cited by 68 publications

References 35 publications

Structure, Genetics and Worldwide Spread of New Delhi Metallo-β-lactamase (NDM): a threat to public health

Structure, Genetics and Worldwide Spread of New Delhi Metallo-β-lactamase (NDM): a threat to public health

Prevalence of VIM- and GIM-producing Acinetobacter baumannii from patients with severe urinary tract infection

Beta-Lactamases Production in Multi-drug Resistant Acinetobacter species Isolated from Different Clinical Specimens

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