Early detection of fungal infection of stored apple fruit with optical sensors – Comparison of biospeckle, hyperspectral imaging and chlorophyll fluorescence

Pieczywek, Piotr M.; Cybulska, Justyna; Szymańska-Chargot, Monika; Siedliska, Anna; Zdunek, Artur; Nosalewicz, Artur; Baranowski, Piotr; Kurenda, Andrzej

doi:10.1016/j.foodcont.2017.10.013

Cited by 48 publications

(23 citation statements)

References 18 publications

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“…biospeckle imaging of infected fruits from the day of pathogen inoculation showed that development of necrosis and the onset of the defence mechanism occurred even on the first day of fungal disease. Biospeckle activity undergoes changes during fungal infection, and the initial visual signs of infection are related to suppression of the photosynthesis rate and cessation of this process at the later stages 32 . In our studies, the first macroscopically clear symptoms of disease appeared on day 4 of infection.…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemical studies on the distribution of arabinogalactan proteins (AGPs) as a response to fungal infection in Malus x domestica fruit

Leszczuk

Pieczywek

Gryta

et al. 2019

Sci Rep

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Arabinogalactan proteins (AGPs) are cell components implicated in plant-microbe interactions. Despite the significance of AGPs in response to stress factors, their distribution during development of fungal disease in fruit is unknown. In our work, in situ analysis of AGP arrangement in fruit inoculated with Penicillium spinulosum during the consecutive days of infection development was carried out. For immunolocalization of AGPs, samples were incubated with JIM13, MAC207, LM2, and LM14 antibodies recognizing the AGP carbohydrate moieties. To analyse cell walls without proper action of AGP, an experiment with β-glucosyl Yariv reagent specifically binding AGPs was performed. The results showed an increase of signal fluorescence in the fruit after 16 days of fungal disease. Higher amounts of the examined epitopes were observed in the infection-altered sites of the fruit, in close vicinity to a surface filled by fungal spores. The results indicate that the Yariv reagent treatment induced progress of the fungal disease. Changes in the AGP presence during the fungal disease confirmed their involvement in defence against pathogen attack in fruit.

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Section: Discussionmentioning

confidence: 99%

Immunocytochemical studies on the distribution of arabinogalactan proteins (AGPs) as a response to fungal infection in Malus x domestica fruit

Leszczuk

Pieczywek

Gryta

et al. 2019

Sci Rep

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“…In Ref. [15] the authors used biospeckle analysis to detect the infection in apples by fungal cultures. They have compared with reference methods, such as hyperspectral imaging and chlorophyll fluorescence.…”

Section: Evaluation Of Maize Seeds Treated With Trichodermilmentioning

confidence: 99%

Evaluation of Maize Seeds Treated with Trichodermil® through Biospeckle

Silva¹,

Peixoto²,

Fujii³

et al. 2018

JAST-B

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Abstract:The present work aimed to study the treatment with the bioprotector Trichoderma harzianum on maize seeds immediately after treatment and during storage. Maize seeds were analyzed through germination test, vigor tests (cold test, accelerated aging and aerial length), and the viability of the treated seeds was verified through the biospeckle. Seed treatment is recommended because approximately 90% of crops intended for human and animal feeding are seed-propagated. A pathogen present in the seed results in increased diseases in the adult plant, in addition to introducing pathogens in other areas. So the seed treatment can provide better results than chemicals disease control on plants. Bioprotectors have antagonistic fungi that produce metabolites and enzymes that will avoid the proliferation of phytopathogenic fungi. Among the bioprotectors, Trichoderma spp., used for phytosanitary control of seeds, stands out. The tests were carried out with treatment in order to evaluate the physiological potential caused by the treatment with the bioprotector. The biospeckle experimental runs were performed in parallel with the sanitation test. The germination percentages of 93.0%, 87.5%, and 87.5%, respectively, showed that they were all above the minimum requirement for commercialization, which is 85%. Analyzing the vigor and sanitation tests, it was verified that the best treatment was on day zero. Among the seeds analyzed through biospeckle, using the laser speckle contrast analysis (LASCA) technique, it was possible to identify fungi activity.

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“…Another variable frequently attempted to relate to spectroscopic information is the poor performance of the plant in terms of the photo-efficiency, which is generically named as "stress". In any case, it is not a mainstream procedure that the physiology/photosynthetic process of the plant is directly associated to the spectral information; despite the fact that both chlorophyll fluorescence and remote sensing are quite established techniques, only lately this field is gaining attention (e.g., [22,[29][30][31][32]). This has mainly taken place in the context of passive remote sensing targeting the analysis of sun induced fluorescence features (e.g., [33]), which are a precursor sign of plant stress and reduced photosynthetic activity.…”

Section: Introductionmentioning

confidence: 99%

Photophysiology and Spectroscopy of Sun and Shade Leaves of Phragmites australis and the Effect on Patches of Different Densities

Stratoulias

Tóth

2020

Remote Sensing

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Remote sensing of vegetation has largely been revolving around the measurement of passive or active electromagnetic radiation of the top of the canopy. Nevertheless, plants hold a vertical structure and different processes and intensities take place within a plant organism depending on the environmental conditions. One of the main inputs for photosynthesis is photosynthetic active radiation (PAR) and a few studies have taken into account the effect of the qualitative and quantitative changes of the available PAR within the plants canopies. Mostly large plants (trees, shrubs) are affected by this phenomena, while signs of it could be observed in dense monocultures, too. Lake Balaton is a large lake with 12 km2 dense reed stands, some of which have been suffering from reed die-back; consequently, the reed density and stress condition exhibit a vertical PAR variability within the canopy due to the structure and condition of the plants but also a horizontal variability attributed to the reedbed’s heterogeneous density. In this study we investigate the expression of photosynthetic and spectroscopic parameters in different PAR conditions. We concentrate on chlorophyll fluorescence as this is an early-stage indicator of stress manifestation in plants. We first investigate how these parameters differ across leaf samples which are exposed to a higher degree of PAR variability due to their vertical position in the reed culm (sun and shade leaves). In the second part, we concentrate on how the same parameters exhibit in reed patches of different densities. We then look into hyperspectral regions through graphs of coefficient of determination and associate the former with the physiological parameters. We report on the large variability found from measurements taken at different parts of the canopy and the association with spectral regions in the visible and near-infrared domain. We find that at low irradiance plants increase their acclimation to low light conditions. Plant density at Phragmites stands affects the vertical light attenuation and consequently the photophysiological response of basal leaves. Moreover, the hyperspectral response from the sun and shade leaves has been found to differ; charts of the coefficient of determination indicate that the spectral region around the red-edge inflection point for each case of sun and shade leaves correlate strongly with ETRmax and α. When analysing the data cumulatively, independent of their vertical position within the stand, we found correlations of R2 = 0.65 (band combination 696 and 651) and R2 = 0.61 (band combination 636 and 642) for the ETRmax and α, respectively.

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Early detection of fungal infection of stored apple fruit with optical sensors – Comparison of biospeckle, hyperspectral imaging and chlorophyll fluorescence

Cited by 48 publications

References 18 publications

Immunocytochemical studies on the distribution of arabinogalactan proteins (AGPs) as a response to fungal infection in Malus x domestica fruit

Immunocytochemical studies on the distribution of arabinogalactan proteins (AGPs) as a response to fungal infection in Malus x domestica fruit

Evaluation of Maize Seeds Treated with Trichodermil® through Biospeckle

Photophysiology and Spectroscopy of Sun and Shade Leaves of Phragmites australis and the Effect on Patches of Different Densities

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