Early detection breast cancer: role of circulating plasma miRNA-21 expression as a potential screening biomarker

Abstract

Aim: To explore the circulating plasma miRNA-21 potential as an early detection biomarker by comparing early-stage breast cancer (BG) and healthy control (HG) in Indonesian population. Materials and methods: The enlisted patients were twenty-six adult female early-stage breast cancer patients (stage 1A, 1B, 2A, and 2B) of Airlangga University Hospital from August until October 2019. Sixteen volunteers were recruited as matched healthy subjects. MiRNA-21 expression was quantified by plasma qRT-PCR. Data analysis performed using IBM SPSS Statistics v.24. MiRNA-21 cutoff , sensitivity, and specificity were analyzed using receiver operating characteristic (ROC) curve. Results: The subject includes 26 BG and 16 HG subjects. The miRNA-21 expression in BG group was 3.933 (1.181-11.794) and 0.905 (0.164-4.532) in HG group (4.34 folds; p=0.001), with 1.66 cutoff (92.3% sensitivity; 81.2% specificity). MiRNA-21 expression separated analysis in HG showed a 0.578 times lower expression in menopause subjects [0.651 (0.164-0.414)] compared to pre-menopause [(1.123 (0.758-4.532); p=0.031]. Yet, in BG group 1.729 times higher miRNA-21 expression was observed in menopause subjects (6.021±3.583) compared to pre-menopause subjects (3.500±1.517;p=0.022). Conclusion: Circulating miRNA-21 expression is a potential biomarker for early detection of breast cancer and might act as a breast cancer risk predictor.

Potential utility of miRNAs for liquid biopsy in breast cancer

“…Based on recent studies miR-21 represents a key target for most scientists in terms of its dysregulated circulating levels in BC. Indeed, a number of studies have demonstrated that c-miR-21 levels are found to be significantly augmented in BC patients compared with the healthy controls ( 90 , 123 , 127 , 144 , 145 , 150 , 162 , 167 , 169 , 173 , 177 , 185 , 186 , 191 , 201 , 204 , 207 , 213 ). Interestingly, both– miR-21-3p and miR-21-5p are found to be upregulated in BC ( 177 ).…”

Diagnostic and Predictive Value of Liquid Biopsy-derived Exosome miR-21 for Breast Cancer: A Meta-analysis

“…Type of sample: of the nine included studies, four studies [4,16,26, 28] had serum-derived samples and three studies [22,24,25] had plasma-derived samples, and the results of meta-analysis showed: In serum group, Combined sensitivity and speci city were 0.87 [ 95% CI (0.80, 0.93)] and 0.74 [ 95% CI ( 0.62, 0.84)];Combined sensitivity and speci city in the plasma group were 0.86 [ 95% CI ( 0.66, 0.97)] and 0.83 [ 95% CI (0.67, 0.93)], respectively; this result suggests that samples of serum origin have higher sensitivity and lower speci city compared to plasma. Whether to combine: of the nine included studies, nine studies [4,16,[22][23][24][25][26][27][28] reported data on miR-21 alone for the diagnosis of breast cancer and three studies [25][26][27] reported data on miR-21 combined with other miRNAs for the diagnosis of breast cancer, and the meta-analysis showed that the combined sensitivity and speci city of miR-21 alone for breast cancer diagnosis was 0.91 [95% CI (0.86, 0.95)] and 0.85 [95% CI (0.77, 0.91)];the combined sensitivity and speci city of combined diagnosis were 0.97 [95% CI (0.92, 0.99)] and 0.92 [95% CI (0.73, 0.99)];This results suggest that sensitivity and speci city of miR-21 combined with other miRNAs in the diagnosis of breast cancer is superior to that of the diagnosis alone.…”

“…The case group was 1491 breast cancer patients and the control group was 509 healthy subjects, basically all female, with an age range of 26 to 80 years. A xed-effects model was used in R 4.0.1 software, and the results regarding the nine studies[4, 16,[22][23][24][25][26][27][28] were as follows: with a combined sensitivity of 0.91 [95% CI (0.86, 0.95)], combined speci city of 0.85 [95% CI (0.77, 0.91)], DOR of 56.62 [ 95% CI (21.00, 184.83) ], NLR of 0.11 [95% CI ( 0.05, 0.18) ], PLR of 6.35 [* --: No speci c data RT-PCR: quantitative Real-time PCR TNBC: Triple negative breast cancer BC breast cancer…”

CircRNA protein tyrosine phosphatase receptor type a suppresses proliferation and induces apoptosis of lung adenocarcinoma cells via regulation of microRNA-582-3p

“…Iorio et al [ 15 ] identified 17 upregulated miRNAs, and 19 downregulated miRNAs in gastric cancerous tissues. miR-21 expression was up-regulated in breast cancer [ 16 ]. A study by Huang et al .…”