Early behavioral and histological outcomes following a novel traumatic partial nerve lesion

Hulata, David; Hughes, William F.; Shott, Susan; Gonzalez, Mark H.; Kerns, James M.

doi:10.1016/j.jneumeth.2008.05.002

Cited by 7 publications

(12 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The last 6 weeks of recovery resembled a plateau period with little additional recovery to final PMR values from 67% to 75%, with no significant group differences. These comparisons were very similar to our previous results, 12 comparing three different types of tibial Fig. 2 The gross appearance of the left sciatic nerve from the (A) control group shows the tibial nerve suture site (arrow) and the distal fat pad in the left popliteal space.…”

Section: Discussionsupporting

confidence: 88%

See 1 more Smart Citation

Comparison of Potential Nerve Scar Agents in the Rat Model

Mossad

Shah

Amirouche

et al. 2016

J Reconstr Microsurg Open

View full text Add to dashboard Cite

In the setting of nerve injury, crush injuries maintain epineural and perineural integrity which facilitates recovery while transection injuries introduce a defect which hinders the degree of functional recovery. In addition, transection injuries show an increased amount of collagen or scar formation both around the site of injury and intraneurally distal to the site of injury. Clinically, extraneural scarring surrounding the transection causes neural tissue to adhere to adjacent tissues, compress the nerve, and compromise the blood supply. These tissue alterations can ultimately augment the nerve defect or limit healing potential.2 Surgical, pharmacological, and radiative therapies have been attempted to address the defect and scar. AbstractBackground An animal nerve scar model that can be used to identify potential therapies in the treatment of nerve scarring in patients has yet to be defined. The purpose of this study was to create an inexpensive, reproducible, and reliable nerve scar model in the rat. Methods Thirty rats underwent left tibial nerve transection and repair and were divided into three groups: 10 controls with transection and repair alone, 10 receiving topical application of talc powder onto the repair, and 10 receiving topical application of tetracycline onto the repair. Outcome measures included weekly extensor postural thrust (EPT) testing for 12 weeks, endpoint gastrocnemius weight ratios (left vs right), and histological analysis.Results The tetracycline group showed a significant difference in EPT testing at week 6 postoperatively (p ¼ 0.007) and had the lowest average EPT value at each time point over 12 weeks. Each group displayed a plateau in recovery, with the control and talc groups reaching plateau 1 week earlier (week 6) than the tetracycline group (week 7). At 12 weeks postoperatively, there was no significant difference in EPT testing (p ¼ 0.301) or gastrocnemius weight ratios (p ¼ 0.802). Histological analysis showed substantial nerve regeneration in the control group, an inflammatory response in the epineurium of the talc and tetracycline groups, but no migration of inflammation or interference of nerve regeneration in the endoneurial compartment. Conclusion These agents, and more importantly, this animal model are inadequate for developing a nerve scar. Identifying a better animal with more connective tissue should be further explored.

show abstract

Section: Discussionsupporting

confidence: 88%

“…At least 10 trials were conducted for each hind limb, and the mean values were used to calculate (left divided by right) a percent motor recovery (PMR). 12 The testing was performed primarily by one designated person (S. S.).…”

Section: Extensor Postural Thrustmentioning

confidence: 99%

Comparison of Potential Nerve Scar Agents in the Rat Model

Mossad

Shah

Amirouche

et al. 2016

J Reconstr Microsurg Open

View full text Add to dashboard Cite

show abstract

“…Because the degenerating fibers involve fragmentation, 22 a certain portion of the fiber loss is not visible. 23 Although beyond the scope of the present study, others have considered the various mechanisms and temporal sequences involved in axonal injury. 31 A consistent finding in our study was that the duration of sciatic nerve block with local anesthetics is longer in diabetic rats versus nondiabetic rats.…”

Section: Discussionmentioning

confidence: 98%

“…Such profiles must be distinguished from those that are truly abnormal or suggestive of neuropathy. Based on our prior experience, 23,24 and that of others, 25 we determined 3 different types of abnormal profiles suggestive of axonal damage, listed in order of highest severity first: (1) axon and myelin degeneration: these profiles called myelin figures have both the myelin and axoplasm combined in a dark-staining mass suggestive of more advanced degeneration; (2) axon degeneration with myelin thinning: these profiles occur in greater numbers and exhibit both a thin myelin sheath and an enlarged axoplasm that is either flocculent or absent; (3) axon degeneration with normal myelin: these profiles may have a normal appearance of the myelin sheath but have dark staining of the axoplasm suggestive of early degeneration. Profiles of myelinated axons that fit into these 3 categories were identified by oil immersion light microscopy, counted from the entire nerve section, and expressed as a percentage of the total number of fibers in each sciatic nerve.…”

Section: Histologymentioning

confidence: 99%

Local Anesthetic Sciatic Nerve Block and Nerve Fiber Damage in Diabetic Rats

Buvanendran

Williams

Wagenaar³

et al. 2010

Regional Anesthesia and Pain Medicine

Self Cite

View full text Add to dashboard Cite

The duration of sciatic nerve block with local anesthetics is longer in diabetic compared with nondiabetic rats. A small, but statistically significant, increase in nerve damage occurred in diabetic rats after nerve block with ropivacaine alone or when duration of lidocaine block was extended with clonidine. These findings may have implications for dosing of local anesthetics in diabetic patients undergoing regional analgesia with nerve blocks.

show abstract

“…We have presented a third experimental model called the partial nerve lesion, 29 where one could compare injured and normal fibers distally in discrete zones of the same nerve (e.g., tibial). In that study, we also showed that the test for extensor postural thrust (EPT) is better suited than walking track analyses 27,30,31 in terms of less variability and stronger correlation with other outcome measures.…”

mentioning

confidence: 99%

Histological Assessment of Wallerian Degeneration of the Rat Tibial Nerve Following Crush and Transection Injuries

Kerns

Walter

Patetta

et al. 2020

J Reconstr Microsurg

Self Cite

View full text Add to dashboard Cite

Background Wallerian degeneration (WD) following peripheral nerve injury (PNI) is an area of growing focus for pharmacological developments. Clinically, WD presents challenges in achieving full functional recovery following PNI, as prolonged denervation of distal tissues for an extended period of time can irreversibly destabilize sensory and motor targets with secondary tissue atrophy. Our objective is to improve upon histological assessments of WD. Methods Conventional methods utilize a qualitative system simply describing the presence or absence of WD in nerve fibers. We propose a three-category assessment that allows more quantification: A fibers appear normal, B fibers have moderate WD (altered axoplasm), and C fibers have extensive WD (myelin figures). Analysis was by light microscopy (LM) on semithin sections stained with toluidine blue in three rat tibial nerve lesion models (crush, partial transection, and complete transection) at 5 days postop and 5 mm distal to the injury site. The LM criteria were verified at the ultrastructural level. This early outcome measure was compared with the loss of extensor postural thrust and the absence of muscle atrophy. Results The results showed good to excellent internal consistency among counters, demonstrating a significant difference between the crush and transection lesion models. A significant decrease in fiber density in the injured nerves due to inflammation/edema was observed. The growth cones of regenerating axons were evident in the crush lesion group. Conclusion The ABC method of histological assessment is a consistent and reliable method that will be useful to quantify the effects of different interventions on the WD process.

show abstract

Early behavioral and histological outcomes following a novel traumatic partial nerve lesion

Cited by 7 publications

References 46 publications

Comparison of Potential Nerve Scar Agents in the Rat Model

Comparison of Potential Nerve Scar Agents in the Rat Model

Local Anesthetic Sciatic Nerve Block and Nerve Fiber Damage in Diabetic Rats

Histological Assessment of Wallerian Degeneration of the Rat Tibial Nerve Following Crush and Transection Injuries

Contact Info

Product

Resources

About