E-rosette formation of fresh versus frozen-thawed lymphocytes from leukemic patients and normal individuals

Polizzi, Gaetano J.; Cohen, E. G. D.; Gregory, Shirley G.

doi:10.1016/0011-2240(78)90008-1

Cryobiology

1978

DOI: 10.1016/0011-2240(78)90008-1

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E-rosette formation of fresh versus frozen-thawed lymphocytes from leukemic patients and normal individuals

Gaetano J. Polizzi

E. G. D. Cohen

Shirley G. Gregory

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Cited by 6 publications

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“…The effects of cryopreservation on the peripheral blood mononuclear cells (PBMC) of patients with periodontal disease have not been investigated despite numerous studies evaluating the immunological status of cryopreserved cells of patients with several malignant diseases, such as leukemia (28), melanoma (10,11,29), breast and colon carcinomas (43), and sarcoma (31). The immunological competence of periodontitis patients is characterized by the functional activity of their immune cells (8,27,32,37).…”

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confidence: 99%

Studies of proliferative responses by long-term-cryopreserved peripheral blood mononuclear cells to bacterial components associated with periodontitis

Miller¹,

Hickey²,

D'Alesandro³

et al. 1996

Clin Diagn Lab Immunol

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Freezing techniques provide a means for repeating and extending immunological assays with frozen aliquots of an individual's peripheral blood mononuclear cell fraction. Lymphocytes which are stored frozen for a limited time retain their ability to respond to polyclonal B-cell activators, mitogens, and antigens of dental interest. Our studies extend these previous findings by determining lymphocyte functional activity following frozen storage for up to 100 weeks. In addition, the autologous immune response was measured by spontaneous lymphocyte proliferation following 0, 1, 10, 40, and 60 weeks of frozen storage. Peak responses for all individuals occurred at day 7 of incubation. The lymphocyte proliferative response to the superantigens toxic shock syndrome toxin-1 (TSST-1) and Staphylococcus enterotoxin A (SEA) were not changed after 100 weeks of frozen storage. Maximum responses varied among the individuals but occurred at equivalent stimulator concentrations. However, slopes generated from data obtained following 0, 4, 13, 20, 30, 50, 88, and 100 weeks of frozen storage showed no significant deviation from zero (P > 0.05) for all individuals tested. After 100 weeks of storage, the total changes in proliferative activity (counts per minute per week) were ؊2.1% ؎ 16.8% and ؊5.5% ؎ 17.0% for TSST-1 and SEA, respectively. The lymphocyte proliferative responses to pokeweed mitogen, concanavalin A, and sonicates of two periodontal pathogens (Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans) following frozen storage were similar to those with TSST-1 and SEA. These results indicate that peripheral blood mononuclear cells stored frozen may serve as appropriate controls to monitor changes in the disease state during long-term periodontal treatment.

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mentioning

confidence: 99%

Studies of proliferative responses by long-term-cryopreserved peripheral blood mononuclear cells to bacterial components associated with periodontitis

Miller¹,

Hickey²,

D'Alesandro³

et al. 1996

Clin Diagn Lab Immunol

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The maintenance of B-cell and T-cell function in frozen and stored human lymphocytes

et al. 1981

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The objective of this study was to identify and test a convenient means for long-term storage of lymphocytes taken from clinically characterized patients without losing B- or T-cell function. Accordingly, peripheral blood lymphocytes were frozen and stored, and portions of each sample were subsequently assayed for T-cell blastogenic response and B-cell Jerne plaquing at various time intervals after freezing. A comparison of the cell counts of fresh and frozen cultures indicated that cell were recovered after freezing. Furthermore, these cells showed no significant differences in (i) cell viability; (ii) blastogenic response to antigens of Actinomyces maeslandii, Bacteroides melaninogenicus, Fusobacterium nucleatum, and tetanus toxoid; (iii) blastogenic response to phytohemagglutinin and pokeweed mitogen; and (iv) polyclonal B-cell response to pokeweed mitogen as measured by the direct Jerne plaque assay. The retained blastogenic and plaquing responses seen in frozen cultures indicated the maintenance of both T-cell and B-cell function, respectively. This is the first reported demonstration of Jerne plaquing of normal human lymphocytes after freezing. It appears that freezing techniques provide a means for repeating and extending both T- and B-cell assays using frozen stored portions of the same cell samples.

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Effects of cryopreservation on the mononuclear cells of patients with lung cancer and normal controls

Venkataraman¹,

Westerman²

1987

Cryobiology

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E-rosette formation of fresh versus frozen-thawed lymphocytes from leukemic patients and normal individuals

Cited by 6 publications

References 8 publications

Studies of proliferative responses by long-term-cryopreserved peripheral blood mononuclear cells to bacterial components associated with periodontitis

Studies of proliferative responses by long-term-cryopreserved peripheral blood mononuclear cells to bacterial components associated with periodontitis

The maintenance of B-cell and T-cell function in frozen and stored human lymphocytes

Effects of cryopreservation on the mononuclear cells of patients with lung cancer and normal controls

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