E. coli O104:H4 outbreak and haemolytic–uraemic syndrome

Borgatta, Bárbara; Kmet-Lunaček, N.; Rello, Jordi

doi:10.1016/j.medin.2011.11.022

Cited by 29 publications

(13 citation statements)

References 28 publications

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“…However, recent data demonstrated that treatment with ciprofloxacin reduced the risk of HUS in patients infected with the 2011 German O104 STEC strain (116). These data are supported by a recent meta-analysis of studies between 1980 and 2011 (117). Despite this, the decision to treat a patient with STEC infection with antimicrobials remains controversial.…”

Section: Escherichia Colimentioning

confidence: 94%

Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Bacterial Gastroenteritis

2015

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SUMMARY Bacterial gastroenteritis is a disease that is pervasive in both the developing and developed worlds. While for the most part bacterial gastroenteritis is self-limiting, identification of an etiological agent by bacterial stool culture is required for the management of patients with severe or prolonged diarrhea, symptoms consistent with invasive disease, or a history that may predict a complicated course of disease. Importantly, characterization of bacterial enteropathogens from stool cultures in clinical laboratories is one of the primary means by which public health officials identify and track outbreaks of bacterial gastroenteritis. This article provides guidance for clinical microbiology laboratories that perform stool cultures. The general characteristics, epidemiology, and clinical manifestations of key bacterial enteropathogens are summarized. Information regarding optimal specimen collection, transport, and processing and current diagnostic tests and testing algorithms is provided. This article is an update of Cumitech 12A (P. H. Gilligan, J. M. Janda, M. A. Karmali, and J. M. Miller, Cumitech 12A, Laboratory diagnosis of bacterial diarrhea, 1992).

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Section: Escherichia Colimentioning

confidence: 94%

Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Bacterial Gastroenteritis

2015

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“…Despite the low platelet level, a diagnosis of thrombotic thrombocytopenic purpura was not supported, especially in the absence of neurological symptoms [11]. Two red cell transfusions were given, and azithromycin treatment was commenced in accordance with our local protocol [12].…”

Section: Case Reportmentioning

confidence: 99%

CFH gene mutation in a case of Shiga toxin-associated hemolytic uremic syndrome (STEC-HUS)

et al. 2015

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We report a case of STEC-HUS with a quantitative CFH defect caused by a mutation of the CFH gene. To the best of our knowledge, very few cases of STEC-HUS with complement gene mutation have been reported, but none to date with a CFH mutation. We therefore suggest that complement analyses be performed in patients diagnosed with STEC-HUS in association with low C3 levels, especially in patients presenting with severe or unexpected clinical symptoms.

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“…Although most E. coli are harmless, some strains can cause diverse gastrointestinal tract diseases, such as diarrhea and emesis, and even colorectal cancer (CRC) in human and other animals (Guiral et al 2011;Levine and Edelman 1984;Pakalniskiene et al 2009;Arthur et al 2012). This opportunistic pathogens entering into internal environment can also cause hematologic malignancies, such as hemolytic-uremic syndrome, early onset neonatal sepsis, and so on (Borgatta et al 2012;Rasko et al 2011;Stoll et al 2011). There are 1.4~2.5 million deaths due to pathogenic E. coli each year (Murray et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Recombinant outer membrane protein A induces a protective immune response against Escherichia coli infection in mice

Guan

Wang

et al. 2015

Appl Microbiol Biotechnol

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Pathogenic Escherichia coli (E. coli) is an important infectious Gram-negative bacterium causing millions of death every year. Outer membrane protein A (OmpA) has been suggested as a potential vaccine candidate for conferring protection against bacterial infection. In this study, a universal vaccine candidate for E. coli infection was developed and evaluated. Bioinformatics analysis revealed the OmpA protein from E. coli shares 96~100%, 90~94%, and 45% identity with Shigella, Salmonella, and Pseudomonas strains, respectively. The ompA gene was cloned from the genomic DNA of E. coli, and then the OmpA protein was expressed in BL21 (DE3) using the auto-induction method. The recombinant OmpA (rOmpA) protein had an average molecular weight of 36 kDa with the purity of 93.5%. Immunological analysis indicated that the titers of anti-rOmpA sera against rOmpA and whole cells were 1:642,000 and 1:140,000, respectively. Moreover, rOmpA not only conferred a high level of immunogenicity to protect mice against the challenge of E. coli, but also generated cross-protection against Shigella and Salmonella. The anti-rOmpA sera could enhance the phagocytic activity of neutrophils against E. coli. The survive ratios of mice immunized with rOmpA and PBS were 50% and 20% after 48 h post-challenge, indicating mice were protected from E. coli infection after immunization with rOmpA. All these results clearly indicate that rOmpA may be a promising candidate for the development of a subunit vaccine to prevent E. coli infection.

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E. coli O104:H4 outbreak and haemolytic–uraemic syndrome

Cited by 29 publications

References 28 publications

Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Bacterial Gastroenteritis

Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Bacterial Gastroenteritis

CFH gene mutation in a case of Shiga toxin-associated hemolytic uremic syndrome (STEC-HUS)

Recombinant outer membrane protein A induces a protective immune response against Escherichia coli infection in mice

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