E.coli Nissle increases transcription of flagella assembly and formate hydrogenlyase genes in response to colitis

Dacquay, Louis; Tsang, Derek; Chan, David; Parkinson, John; Philpott, Dana J.; McMillen, David R.

doi:10.1080/19490976.2021.1994832

Cited by 3 publications

(2 citation statements)

References 42 publications

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“…Intestinal inflammation in patients with CD provides favorable conditions for the specific colonization of AIEC. Studies have shown that engraftment of AIEC through adhesion is realized only in DSS-induced colitis, and broad-spectrum antibiotics destroy the original intestinal flora in mice or induce immune deficiency, such as IL-10 loss, in knockout mice[ 93 ]. In CD, the concentration of the intestinal mucosa containing AIEC is far higher than that in the area of inflammation intestinal mucosa.…”

Section: Aiec In CDmentioning

confidence: 99%

Role of adherent invasive Escherichia coli in pathogenesis of inflammatory bowel disease

Duan¹,

Dai²,

Wu³

2022

World J Clin Cases

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Gut microbiota imbalances play an important role in inflammatory bowel disease (IBD), but no single pathogenic microorganism critical to IBD that is specific to the IBD terminal ileum mucosa or can invade intestinal epithelial cells has been found. Invasive Escherichia coli ( E. coli) adhesion to macrophages is considered to be closely related to the pathogenesis of inflammatory bowel disease. Further study of the specific biological characteristics of adherent invasive E. coli (AIEC) may contribute to a further understanding of IBD pathogenesis. This review explores the relationship between AIEC and the intestinal immune system, discusses the prevalence and relevance of AIEC in Crohn's disease and ulcerative colitis patients, and describes the relationship between AIEC and the disease site, activity, and postoperative recurrence. Finally, we highlight potential therapeutic strategies to attenuate AIEC colonization in the intestinal mucosa, including the use of phage therapy, antibiotics, and anti-adhesion molecules. These strategies may open up new avenues for the prevention and treatment of IBD in the future.

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Section: Aiec In CDmentioning

confidence: 99%

Role of adherent invasive Escherichia coli in pathogenesis of inflammatory bowel disease

Duan¹,

Dai²,

Wu³

2022

World J Clin Cases

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“…de-repressed) gene transcriptome-wide ( Fig. 5g ), with the only other significant changes arising in genes whose expression has been linked to flagellar gene transcription 58,59 .…”

Section: Resultsmentioning

confidence: 99%

Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleases

Wiegand,

Hoffmann,

Walker

et al. 2023

Preprint

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Transposon-encodedtnpBgenes encode RNA-guided DNA nucleases that promote their own selfish spread through targeted DNA cleavage and homologous recombination1–4. This widespread gene family was repeatedly domesticated over evolutionary timescales, leading to the emergence of diverse CRISPR-associated nucleases including Cas9 and Cas125,6. We set out to test the hypothesis that TnpB nucleases may have also been repurposed for novel, unexpected functions other than CRIS-PR-Cas. Here, using phylogenetics, structural predictions, comparative genomics, and functional assays, we uncover multiple instances of programmable transcription factors that we name TnpB-like nuclease-dead repressors (TldR). These proteins employ naturally occurring guide RNAs to specifically target conserved promoter regions of the genome, leading to potent gene repression in a mechanism akin to CRISPRi technologies invented by humans7. Focusing on a TldR clade found broadly inEnterobacteriaceae, we discover that bacteriophages exploit the combined action of TldR and an adjacently encoded phage gene to alter the expression and composition of the host flagellar assembly, a transformation with the potential to impact motility8, phage susceptibility9, and host immunity10. Col-lectively, this work showcases the diverse molecular innovations that were enabled through repeated exaptation of genes encoded by transposable elements, and reveals that RNA-guided transcription factors emerged long before the development of dCas9-based editors.

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TnpB homologues exapted from transposons are RNA-guided transcription factors

Wiegand,

Hoffmann,

Walker

et al. 2024

Nature

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E.coli Nissle increases transcription of flagella assembly and formate hydrogenlyase genes in response to colitis

Cited by 3 publications

References 42 publications

Role of adherent invasive Escherichia coli in pathogenesis of inflammatory bowel disease

Role of adherent invasive Escherichia coli in pathogenesis of inflammatory bowel disease

Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleases

TnpB homologues exapted from transposons are RNA-guided transcription factors

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